Abstract: The aim of this study was to investigate the effect of resveratrol on the transcription of inflammatory factors in heat stress (HS)-induced goat small intestinal epithelial cells(GIE cells). The fourth-generation well-growing GIE cells were used for the experiment and were divided into five groups: blank control group (37 ℃), heat stress group (42 ℃), and 42 ℃ heat stress adding resveratrol groups (5, 15, 30 μmol·L^-1); 6 hours' heat stress treatment was carried out after 6 hours' pretreatment with resveratrol supplementation. The resveratrol cytotoxicity was detected by CCK-8 method; the effect of resveratrol on the secretion of cytokines was measured by ELISA kits in HS-induced GIE cells; biochemical method was adopted to detect the effect of resveratrol on antioxidant properties of HS-induced GIE cells. Gene transcriptions of IL-1β, TNF-α, IL-8, HSP70 and NF-κB were detected by RT-PCR in HS-induced GIE cells, respectively. The results showed that resveratrol with different concentrations (5, 15, 30 μmol·L^-1) could increase GSH-Px, SOD and T-AOC activities in HS-induced GIE cells, and extremely significantly decrease MDA content of lipid peroxide (P<0.01); inhibit the secretion of IL-1β, IL-2, IFN-γ and TNF-α in GIE cells induced by heat stress, and extremely significantly reduce the gene transcription levels of IL-1β, TNF-α, IL-8, HSP70 and NF-κB in inflammatory model cells (P<0.01). These results demonstrated that resveratrol could regulate the expression of HSP70 gene and the production and expression of inflammatory factors in the inflammatory model of GIE cells induced by heat stress, promote the activity of antioxidant enzymes and have significant anti-inflammatory activities. Its anti-inflammatory effect might be related to the inhibition of NF-κB signaling pathway.

Key words: resveratrol, goat small intestinal epithelial cells, heat stress, HSP70, anti-inflammatory, NF-κB