绵羊Fsp27基因组织表达、多态性及其与不同绵羊品种尾脂沉积能力的关联性分析

doi:10.11843/j.issn.0366-6964.2020.05.007

Abstract

Abstract: The aim of this study was to investigate tissue expression profiles and polymorphism of Fsp27 gene in sheep (Ovis aries) and their association with tail fat deposition of different sheep breeds. The qRT-PCR was applied to detect the expression level of Fsp27 gene in different tissues of Altay sheep in adequate nutrition stage, and its expression levels in tail fat of Altay sheep in adequate and deficient nutrition stages, then the PCR-SSCP combined with sequencing were used to detect polymorphism of Fsp27 gene in 5 sheep breeds with different tail fat deposition ability and their association with tail fat deposition in sheep were analyzed. The results showed that Fsp27 gene was expressed at a high level in tail fat of Altay sheep in adequate nutrition stage and extremely higher than those in the other tissues (P<0.01), and expressed at a relatively high level in perirenal fat and subcutaneous fat which was extremely higher than those in heart, liver, spleen, lung, kidney, stomach, intestine, skin and skeletal muscle tissues (P<0.01), but expressed at a very low level in heart, liver, spleen, lung, kidney, stomach, intestine, skin and skeletal muscle tissues, and there were no significant difference among them (P>0.05). In addition, the expression level of Fsp27 gene in tail fat tissue of Altay sheep in adequate nutrition stage was significantly higher than that in deficient nutrition stage (P<0.01). Twenty-five SNPs were detected in Fsp27 gene sequence of sheep with different tail fat deposition ability, G/A mutation at g. 16771741 site in exon3 and C/T at g. 16774969 site in exon5 were missense mutations, and they were significantly associated with tail fat deposition ability of sheep. G was dominant allele in Altay sheep, Small Tail Han sheep and Hu sheep at g. 16771741 site, the allele frequency was 86.8%, 83.7% individuals and 85.7%, respectively, but only 30.3% and 11.1% individuals of Chinese Merino and Suffolk sheep were G allele at this site. T was dominant allele in Altay sheep at g. 16774969 site, 84.7% were TT genotype, 15.3% individuals were CT genotype, and no CC genotype was detected, and the genotype frequency of TT and CT in Small Tail Han sheep and Hu sheep were 65.9%, 50.4% and 22.7%, 41.1%, respectively, but CC was dominant genotype in Chinese Merino and Suffolk sheep, their frequency were 97.4% and 69.4%, and no TT genotype was detected. These results indicated that Fsp27 gene expressed at a high level in tail fat tissue of Altay sheep, and its expression level was closely related with nutrition state of Altay sheep. The G/A mutation at g. 16771741 site in exon3 and C/T mutation at g. 16774969 site in exon5 of Fsp27 gene were highly correlated with tail fat deposition ability of sheep, so could be used to help selecting low fat sheep breed.

Key words: sheep, Fsp27, tail fat, SNP

CLC Number:

S826.2

ZHANG Wei, WANG Shiyin, GAO Li, XU Mengsi, WANG Xinhua, GAN Shangquan. Tissue Expression Profiles and Polymorphism of Fsp27 Gene in Sheep (Ovis aries) and Their Association with Deposition of Tail Fat[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(5): 952-964.

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Tissue Expression Profiles and Polymorphism of Fsp27 Gene in Sheep (Ovis aries) and Their Association with Deposition of Tail Fat

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