猪胚胎早期卵裂时间与其发育潜能关系的初步研究

doi:10.11843/j.issn.0366-6964.2020.04.010

Abstract

Abstract: This study aimed to explore the effect of first cleavage time on the development potential and relative gene expression levels in parthenogenetic embryos of the pig. The oocytes were extracted from the ovary of a healthy sow for maturation in vitro, the pig parthenogenetic embryos were divided into two groups:early cleavage group(16-22 h) and late cleavage group (26-32 h), their cleavage rate and blastocyst rate were statistically compared. The expression levels of pluripotency-related genes Oct4, Sox2, Klf4 and other apoptosis-related genes Bcl-xl, Bax, Caspase-3 were detected and analyzed. The results showed that 50%-60% cleavage in parthenogenetically activated embryos of the pig occured at 16-22 h and less than 20% after 26 h. The embryos blastocyst development rate which completed the first cleavage before 18 h was 79%. Whereas, the embryos which completed the first cleavage after 42 h were not able to develop to the blastocyst stage. The blastocysts development rate in the early cleavage group of parthenogenetically activated pig embryos was significantly higher compared to the late cleavage group (P<0.05), the expression level of Oct4, Nanog, Sox2, and Klf4 genes in the early cleavage group were significantly higher than those in the late cleavage group (P<0.05). The expression level of Oct4, Sox2, Klf4 genes in the early cleavage group were extremely significantly higher than those in the late cleavage group (P<0.01); While the relative expression level of Bax and Caspase-3 genes in the early cleavage group were extremely significantly lower than those in the late cleavage group (P<0.01); However, the expression level of Bcl-xl as a protective factor was significantly up-regulated compared to the late cleavage embryos (26-32 h, P<0.05). The results indicate that the parthenogenetic activation potential of pigs with earlier first cleavage time is significantly higher than that of the late cleavage embryos. The expression of blastocyst pluripotency-related genes is up-regulated and the expression of apoptosis-related genes is down-regulated. The cleavage time as important parameters can be used to identify developmental potential of pig parthenogenetic activated embryos.

Key words: pig, the first cleavage, developmental potential, pluripotent gene, apoptosis gene

CLC Number:

S828.2

LI Tiantian, XIONG Xin, DUAN Linwei, LI Zhipeng, FU Penghui, XU Yixue, SHI Deshun, LIU Qingyou, CUI Kuiqing. Primary Study on the Effect of Early Cleavage Time on the Developmental Potential of Pig Embryos[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(4): 743-753.

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Primary Study on the Effect of Early Cleavage Time on the Developmental Potential of Pig Embryos

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