围产期小鼠子宫颈中糖皮质激素受体、11β羟基类固醇脱氢酶的表达

doi:10.11843/j.issn.0366-6964.2015.11.024

Abstract

Abstract:

The present study was designed to examine the pattern and cellular localization of 11β-hydroxysteroid dehydrogenase（11β-HSD） enzymes and glucocorticoids receptor（GR） gene expression in the murine cervix during peripartal period，and to gain insight into the role of glucocorticoid（GC） and GR，11β-HSD-1 and 2 in the cervical ripening of peripartal mice.Mice in five groups were from 48 h before birth（-48 h） and-24 h，at term labor（0 h），12 h after birth（+12 h） and +48 h.The dynamic change of the serum corticosterone level was determined by ELISA and immunolocalization of GR，11βHSD-1 and 2 which influence glucocorticoid’s activity in cervix was assayed by immunohistochemistry.The results suggested that the levels of serum corticosterone were significantly increased before the onset of labor，reached the maximum during delivery（P＜0.01）.In addition，the 11βHSD-1，11βHSD-2 and GR protein were localized to the nuclei of cervical luminal epithelia，stromal cells，vascular and cervical smooth muscles.The quotient between mean optical density of 11βHSD-1 and 2 is highest at parturition.Furthermore，the immunoreactivity of GR also reached the peak at parturition（P＜0.01）.These results revealed that 11βHSD-1-regenerated glucocorticoids may act via its receptor to play an important role in the final cervical ripening.

CLC Number:

S857.2

LI Ting-ting，TIAN Gao-qiang，LUO Ming-jiu，HUANG Li-bo，GE Li-jiang. Expression of Glucocorticoids Receptor，11β-Hydroxysteroid Dehydrogenase in the Cervices of Peripartal Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(11): 2097-2103.

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References

［1］McCULLERS D L，HERMAN J P.Adrenocorticosteroid receptor blockade and excitotoxic challenge regulate adrenocorticosteroid receptor mRNA levels in hippocampus［J］.J Neurosci Res，2001，64（3）：277-283.
［2］CHALLIS J R G.Mechanism of parturition and preterm labor［J］.Obstet Gynecol Surv，2000，55（10）：650-660.
［3］SCHÄUBLI M，RITTER N，HÄSSIG M，et al.Progesterone receptors，oestrogen receptor alpha and gulcocorticoid receptors in the bovine intercaruncular uterine wall around parturition［J］.Anim Reprod Sci，2008，103（3-4）：215-227.
［4］BOOS A，KOHTES J，JANSSEN V，et al.Pregnancy effects on distribution of progesterone receptors，oestrogen receptor alpha，glucocorticoid receptors ki-67 antigen and apoptosis in the bovine interplacentomal uterine wall and foetal membranes［J］.Anim Reprod Sci，2006，91（1-2）：55-76.
［5］GU G，GAO Q，YUAN X J，et al.Immunolocalization of adipocytes and prostaglandin E2 and its four receptor proteins EP1，EP2，EP3，and EP4 in the caprine cervix during spontaneous term labor［J］.Biol Reprod，2012，86（5）：1-10.
［6］OSMAN I，YOUNG A，LEDINGHAM M A，et al.Leukocyte density and pro-inflammatory cytokine expression in human fetal membranes，decidua，cervix and myometrium before and during labour at term［J］.Mol Hum Reprod， 2003，9（1）：41-45.
［7］SENNSTRÖM M B，EKMAN G，WESTERGREN-THORSSON G，et al.Human cervical ripening，an inflammatory process mediated by cytokines［J］.Mol Hum Reprod，2000，6（4）：375-381.
［8］MCKAY L I，CIDLOWSKI J A.Molecular control of immune/inflammatory responses：interactions between nuclear factor-κB and steroid receptor-signaling pathways［J］.Endocr Rev，1999，20（4）：435-459.
［9］ECONOMOPOULOS P，SUN M，PURGINA B，et al.Glucocorticoids stimulate prostaglandin H synthase type-2（PGHS-2） in the fibroblast cells in human amnion cultures［J］.Mol Cell Endocrinol，1996，117（2）：141-147.
［10］ZAKAR T，OLSON D M.Dexamethasone stimulates arachidonic acid conversion to prostaglandin E2 in human amnion cells［J］.J Dev Physiol，1989，12（5）：269-272.
［11］ZAKAR T，OLSON D M.Studies on glucocorticoid hormone actions in the regulation of human amnion PGHS［J］.Reprod Fertil Dev，1995，7（3）：517-520.
［12］ZAKAR T，HIRST J J，MIJOVIC J E，et al.Glucocorticoids stimulate the expression of prostaglandin endoperoxide H synthase-2 in amnion cells［J］.Endocrinology， 1995，136（4）：1610-1619.
［13］CHALLIS J R G，GIBB W.Control of parturition［J］.Prenat Neonat Med，1996，1：283.
［14］POTESTIO F A，ZAKAR T，OLSON D M.Glucocorticoids stimulate prostaglandin synthesis in human amnion cells by a receptor-mediated mechanism［J］.J Clin Endocrinol Metab，1988，67（6）：1205-1210.
［15］CHALLIS J R，MATTHEWS S G，VANMIER C，et al.Current topic：the placental corticotrophin-releasing hormone-adrenocorticotrophin axis［J］.Placenta，1995，16（6）：481-502.
［16］KEELAN J A，COLEMAN M，MITCHELL M D.The molecular mechanisms of term and preterm labor：recent progress and clinical implications［J］.Clin Obstet Gynecol，1997，40（3）：460-478.
［17］董彦亮.妊娠期子宫颈成熟和催熟的研究［J］.徐州医学院学报，1985，5（4）：64-69.
DONG Y L.Study on cervical ripening and mature during pregnancy［J］.Acta Academiae Medicinae Xuzhou，1985，5（4）：64-69.（in Chinese）
［18］OLSON D M，SKINNER K，CHALLIS J R.Prostaglandin output in relation to parturition by cells dispersed from human intrauterine tissues［J］.J Clin Endocrinol Metab，1983，57（4）：694-699.
［19］SKINNER K A，CHALLIS J R.Changes in the synthesis and metabolism of prostaglandins by human fetal membranes and decidua at labor［J］.Am J Obstet Gynecol，1985，151（4）：519-523.
［20］MITCHELL M D，ROMERO R J，EDWIN S S，et al.Prostaglandins and parturition［J］.Reprod Fert Dev，1995，7（3）：623-632.
［21］NARUMIYA S，SUGIMOTO Y，USHIKUBI F.Prostanoid receptors：structures，properties，and functions［J］.Physiol Rev，1999，79（4）：1193-1226.
［22］MORIMOTO K，OKU M.Effect of progesterone，cortisol and dehydroepiandrosterone-sulfate on prostaglandin production by cultured human myometrial cells［J］.Nihon Sanka Fujinka Gakkai Zasshi，1995，47（4）：391-397.
［23］MILLER-GRAZIANO C L，FINK M，WU J Y，et al.Mechanisms of altered monocyted prostaglandin E2 production in severely injured patients［J］.Arch Surg，1998，123（3）：293-299.
［24］LONG J A，FOGEL-PETROVIC M，KNIGHT D A，et al.Higher prostaglandin E2 production by dendritic cells from subjects with asthma compared with normal subjects［J］.Am J Respir Crit Care Med，2004，170（5）：485-491.
［25］JOURDAN K B,EVANS T W,GOLDSTRAW P，et al.Isoprostanes and PGE2 production in human isolated pulmonary artery smooth muscle cells：concomitant and differential release［J］.FASEB J，1999，13（9）：1025-1030.

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Expression of Glucocorticoids Receptor，11β-Hydroxysteroid Dehydrogenase in the Cervices of Peripartal Mice

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