Abstract:

This experiment was conducted to study the genetic variation and phylogenetic analysis of Porcine teschovirus (PTV) HB strain isolated from a pig farm in Hebei Province. ST cells and animal infection experiments were conducted after the virus was originated from clinical tissue samples successfully, and the genome sequence of PTV HB strain had been determined, comparative genome analysis was performed with other 42 PTV strains in GenBank. The virus titer was 10^－6.4 TCID₅₀·mL^－1, and the infected pigs showed the same clinical signs and homological changes as natural infected ones after 28 days，and the complete genome sequence was composed of 7 090 nucleotides. Phylogenetic tree analysis demonstrated that PTV HB strain (JQ664746) belonged to serotype 8 and had a very closely genetic relationship with PTV-jilin/2003 strain (GQ293092) isolated from Jilin Province, China. Our results provided an essential reference for investigating the genetic variation of PTV HB strain.