基于重组酶聚合酶扩增快速检测鸡传染性支气管炎病毒的胶体金试纸条

doi:10.11843/j.issn.0366-6964.2025.12.032

Abstract

Abstract: Infectious bronchitis is an acute and highly contagious respiratory disease caused by infectious bronchitis virus (IBV). In order to establish a suitable method for the field detection of IBV, we designed primers and probes targeting M, a conserved gene of IBV, and combined RPA and colloidal gold technology to create an RPA-test strip method for IBV detection within 1 hour. The specificity test demonstrated that the RPA-test strip method only detected IBV, but did not react with the avian influenza virus (AIV), chicken bursal disease virus (IBDV), Newcastle disease virus (NDV), avian leukosis virus (ALV), and goose astrovirus (GAstV). The sensitivity test proved that the lowest detection line of this RPA-test strip method was 8.26 TCID₅₀·mL^-1. Comparison of 31 clinical samples showed that the positive detection rates of this RPA-strip method, qPCR and conventional PCR were 74.2%, 64.5% and 54.8%, respectively. The above data showed that the RPA-strip method for the rapid detection of IBV established in this study is highly specific, sensitive and easy to operate, which provides a new method and technology for the efficient diagnosis of IBV and its immunological prevention and control.

Key words: infectious bronchitis virus, M gene, RPA, colloidal gold immunochromatographic test strip

CLC Number:

S858.315.3

GUO Pengyu, TANG Ye, MA Lihua, MENG Xianchen, ZHAO Zhehong, XIE Quan, LI Tuofan, WAN Zhimin, SHAO Hongxia, QIN Aijian, YE Jianqiang. Colloidal Gold Test Strip for Rapid Detection of Chicken Infectious Bronchitis Virus based on Recombinase Polymerase Amplification[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(12): 6299-6307.

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Colloidal Gold Test Strip for Rapid Detection of Chicken Infectious Bronchitis Virus based on Recombinase Polymerase Amplification

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