Abstract:

Bacteriophages can specifically lyse target bacteria and are usually considered as effective alternatives to antibiotics. In order to screen for bacteriophages that are efficient to lyse Salmonella, and provide experimental data and reference for exploring the prevention and control method of Salmonella. In this study, Salmonella phage was induced and purified by mitomycin C. Phage was evaluated by plaque and electron microscopy observation, host spectrum determination, biological characterization and whole genome sequencing analysis. A broad-spectrum Salmonella long-tail phage was successfully induced, named SP18-108, which could lyse at least 14 different serotypes of Salmonella. The activity is stable at 30-40℃ and pH 4-12.The whole genome analysis showed that the length of the phage was 43 250 bp, and the GC content was 49.96%, the proportion of known coding sequences (CDS) was 52%. Phylogenetic tree analysis showed that phage SP18-108 belonged to the (Siphoviridae). In summary, in this study, we isolated a broad-spectrum Salmonella bacteriophage without lysogenic genes, which providing a good material for further research on Salmonella antibacterial agents.

Key words: Salmonella, phage, biological characteristics, whole genome analysis