黑龙江省地区犊牛腹泻源致病性大肠杆菌流行情况调查、毒力基因及耐药性分析

doi:10.11843/j.issn.0366-6964.2025.07.031

Abstract

Abstract:

Escherichia coli is one of the main pathogens causing diarrhea and death in newborn calves. The pathogenicity, virulence gene carriage, and antibiotic resistance prevalence of Escherichia coli causing diarrhea in calves in Heilongjiang Province were investigated. Two hundred and fifty-two diarrhea samples of calf were collected from 35 herds in 8 cities from January 2020 to July 2023. Bacterial isolation, Gram staining, identification on differential media, biochemical tests, 16S rRNA identification, mouse pathogenicity tests, and drug sensitivity tests were conducted. The presence of 15 virulence genes was detected using PCR. Experimental data were analyzed using the chi-square test method of SPSS 22.0 software to assess the regional prevalence of pathogenic E. coli, as well as factors related to different years and seasons. Results showed that a total of 153 strains of pathogenic E. coli were isolated and identified, of which 49.02% (75/153) carried virulence genes. The highest positive rate was for Irp-2 at 37.25% (57/153), followed by FyuA, K99、F41, F17, STa, HlyA, Stx1, Stx2 and EaeA at 19.61% (30/153), 4.58% (7/153), 4.58% (7/153), 3.92% (6/153), 2.61% (4/153), 3.27% (5/153), 0.65% (1/153), 0.65% (1/153), and 0.65% (1/153), respectively. Drug sensitivity tests indicated that the highest sensitivity rate of the 153 isolated strains was to fosfomycin at 56.21% (86/153), while there was severe resistance to sulfisoxazole, amoxicillin, etc. All strains were resistant to two or more drugs, with some reaching resistance to as many as 11 drugs. Statistical analysis revealed that the isolation rate of pathogenic E. coli in 2020 was significantly higher at 77.59% (45/58) compared to 2021 and 2022 (P < 0.001). Among the four seasons, the summer had the lowest isolation rate of pathogenic E. coli (P < 0.05). And the rate of pathogenic E. coli was statistically different among regions, with the highest in Jixi and the lowest in Qiqihar (P < 0.001). These results indicate that the pathogenic E. coli strains causing calf diarrhea in the Heilongjiang region carry a diverse range of virulence genes and are generally resistant to multiple drugs. The prevalence of pathogenic E. coli is correlated with region, year, and season. This study provides a theoretical basis for the prevention and control of E. coli infections in cattle in the Heilongjiang region.

Key words: calf diarrhea, Escherichia coli, virulence genes, drug resistance, epidemiology

CLC Number:

S852.612

KONG Xianghe, SU Xinyu, ZHONG Yiming, LUZHAO Zixuan, LIAO Xianmao, ZHANG Zhe, ZHANG Xu, GAO Mengmeng, ZHOU Yulong, FAN Huqing. To Investigate the Prevalence, Virulence Genes and Drug Resistance of Pathogenic Escherichia coli Isolated from Calves with Diarrhea in Heilongjiang Province[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(7): 3390-3398.

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药物类别Drug category	抗菌药物Antibacterials	菌株数Number of strains			耐药情况Drug resistance situation
药物类别Drug category	抗菌药物Antibacterials	耐药Resistance	中介Intermediary	敏感Sensitivity	耐药率/%Rate of resistance	敏感率/%Rate of sensitivity
β-内酰胺类β-lactam class	头孢唑林Cefazolin	86	45	22	56.21	14.37
	阿莫西林Amoxicillin	103	16	34	67.32	22.22
	头孢他啶Ceftazidime	93	27	33	60.78	21.57
喹诺酮类Quinolones	左氧氟沙星Levofloxacin	44	43	66	28.75	43.13
	恩诺沙星Enrofloxacin	54	37	62	35.29	40.52
	环丙沙星Ciprofloxacin	72	33	48	47.06	31.35
氨基糖苷类Aminoglycoside	庆大霉素Gentamicin	66	48	39	43.13	25.49
	卡那霉素Kanamycin	71	43	39	46.41	25.49
	链霉素Streptomycin	69	67	17	45.10	11.11
酰胺醇类Amidochols	氯霉素Chloramphenicol	53	98	2	34.64	1.31
磺胺类Sulfanilamide	磺胺异噁唑Sulfisoxazole	115	37	1	75.16	0.65
四环素类Tetracyclines	四环素Tetracycline	107	40	6	69.93	3.92
大环内酯类Macrolides	阿奇霉素Azithromycin	41	41	71	26.80	46.41
多磷类Polyphosphates	磷霉素Fosfomycin	56	11	86	36.60	56.21

统计指标Statistical indicators	样本来源Sample source								X²	P
统计指标Statistical indicators	大庆市Daqing city	哈尔滨市Harbin city	黑河市Heihe city	齐齐哈尔市Qiqihar city	鸡西市Jixi city	绥化市Suihua city	牡丹江市Mudanjiang city	佳木斯市Jiamusi city	X²	P
阳性率(阳性数/总数)Positive rate(positive number/Total)	70.09(75/105)	35.29(12/34)	61.36(27/44)	16.60(2/12)	100.00(6/6)	66.67(4/6)	80.00(4/5)	36.80(7/19)	139.957	< 0.001
阴性率(阴性数/总数)Negative rate(negative number/Total)	29.91(30/105)	64.71(22/34)	38.64(17/44)	83.40(10/12)	0	33.34(2/6)	20.00(1/5)	63.20(12/19)

统计指标Statistical indicators	样本来源(年) Sample source(Year)			X²	P
统计指标Statistical indicators	2020	2021	2022	X²	P
阳性率(阳性数/总数)Positive rate(positive number/Total)	77.59(45/58)	59.46(44/74)	52.38(33/63)	15.530	< 0.001
阴性率(阴性数/总数)Negative rate(negative number/Total)	22.41(13/58)	40.54(30/74)	47.62(30/63)

统计指标Statistical indicators	样本来源Sample source				X²	P
统计指标Statistical indicators	春季Spring	夏季Summer	秋季Autumn	冬季Winter	X²	P
阳性率(阳性数/总数)Positive rate(positive number/Total)	67.5(54/80)	48.81(41/84)	66.00(33/50)	65.79(25/38)	9.756	0.021
阴性率(阴性数/总数)Negative rate(negative number/Total)	32.5(26/80)	51.19(43/84)	34.00(17/50)	34.21(13/38)

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To Investigate the Prevalence, Virulence Genes and Drug Resistance of Pathogenic Escherichia coli Isolated from Calves with Diarrhea in Heilongjiang Province

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