奶牛黄体的形成、溶解及其在维持妊娠中的作用研究进展

doi:10.11843/j.issn.0366-6964.2025.07.005

Abstract

Abstract:

The study of luteal function in dairy cows is of great importance for improving reproductive efficiency. In recent years, despite advancements in dairy production technology, pregnancy rates remain limited by factors such as embryo loss and luteal insufficiency. As a critical temporary organ for pregnancy maintenance, the function of the corpus luteum directly affects reproductive outcomes. An in-depth understanding of the physiological characteristics, formation, and degradation mechanisms of the corpus luteum is essential for reproductive management in dairy cows. This review summarizes the mechanisms of corpus luteum formation and degradation, internal and external factors influencing its function, and related interventions, with a focus on the application of hormonal regulation and biotechnology in maintaining luteal function. The aim is to provide theoretical insights for improving corpus luteum function and enhancing reproductive efficiency.

Key words: corpus luteum, progesterone, prostaglandins, interferon, embryo loss

CLC Number:

S823.3

LI Qingyun, CAO Fengfeng, XING Zhou, LI Zhuoying, TAO Jinzhong. Progress in the Study of the Formation and Lysis of Corpus Luteum in Dairy Cows and Its Role in the Maintenance of Pregnancy[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(7): 3088-3095.

References 60

1	KO C C A , O NER M . A Research on fertility, herd life, milk production and milk quality characteristics of Simmental (Fleckvieh) Cows: 1. Reproduction, herd life and milk production characteristics[J]. Tur J Agri Food Sci Technol, 2023, 11 (12): 2339- 2346.
2	REARTE R , LEBLANC S J , CORVA S G , et al. Effect of milk production on reproductive performance in dairy herds[J]. J Dairy Sci, 2018, 101 (8): 7575- 7584.
3	李雅鑫, 李彤, 祝丽云. 我国规模化奶牛养殖成本效率及影响因素研究[J]. 中国乳业, 2022 (1): 14- 22.
	LI Y X , LI T , ZHU L Y . Research on the cost efficiency and influencing factors of large-scale dairy farming in China[J]. China Dairy, 2022 (1): 14- 22.
4	L O PEZ-GATIUS F , GARCIA-ISPIERTO I . Clinical overview of luteal deficiency in dairy cattle[J]. Animals (Basel), 2022, 12 (15): 1871.
5	DENIS-ROBICHAUD J , OLIVEIRA A P , SICA A , et al. Is prolonged luteal phase a problem in lactating Holstein cows?[J]. J Dairy Sci, 2024, 107 (10): 8582- 8591.
6	RICCI A , CARVALHO P D , AMUNDSON M C , et al. Factors associated with pregnancy-associated glycoprotein (PAG) levels in plasma and milk of Holstein cows during early pregnancy and their effect on the accuracy of pregnancy diagnosis[J]. J Dairy Sci, 2015, 98 (4): 2502- 2514.
7	GRANACI V , FOKSHA V F , KONSTANDOGLO A , et al. Reproductive qualities of dairy cows at different age and levels of milk yield[J]. Scientific Papers. Series D. Animal Science, 2023 (1): 195- 201.
8	张陈珊, 张正红, 张红, 等. AMPK对黄体脂滴代谢与类固醇激素产生的调节作用[J]. 生命科学, 2019, 31 (10): 1041- 1046.
	ZHANG C S , ZHANG Z H , ZHANG H , et al. Regulatory effects of AMPK signaling on the metabolism of lipid droplets and the production of steroid hormones in the corpus luteum[J]. Life Sciences, 2019, 31 (10): 1041- 1046.
9	GARG A , ZIELINSKA A P , YEUNG A C , et al. Luteal phase support in assisted reproductive technology[J]. Nat Rev Endocrinol, 2024, 20 (3): 149- 167.
10	CROWE A D , S A NCHEZ J E M I , MOORE S G , et al. Fertility in seasonal-calving pasture-based lactating dairy cows following timed artificial insemination or timed embryo transfer with fresh or frozen in vitro--produced embryos[J]. J Dairy Sci, 2024, 107 (3): 1788- 1804.
11	INSKEEP E K . 152 Randel Lecture: Luteal regulation and early embryonic loss in postpartum cows: Applications in reproductive management[J]. J Anim Sci, 2019, 97 (Suppl 1): 49.
12	BERISHA B , THAQI G , SCHAMS D , et al. Effect of the gonadotropin surge on steroid receptor regulation in preovulatory follicles and newly formed corpora lutea in the cow[J]. Domest Anim Endocrinol, 2024, 89, 106876.
13	王强龙, 潘阳阳, 闫翠霞, 等. 雌性牦牛主要生殖器官中DBI的表达与定位分析[J]. 畜牧兽医学报, 2022, 53 (1): 169- 178. doi: 10.11843/j.issn.0366-6964.2022.01.017
	WANG Q L , PAN Y Y , YAN C X , et al. Expression and localization of DBI in main reproductive organs of female yaks[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53 (1): 169- 178. doi: 10.11843/j.issn.0366-6964.2022.01.017
14	周应聪, 张同享, 王靖雷, 等. MST1和LATS1在牦牛黄体形成, 维持和退化中的表达及HCG对其表达的影响[J]. 农业生物技术学报, 2024, 32 (8): 1844- 1856.
	ZHOU Y C , ZHANG T X , WANG J L , et al. The Expression of MST1 and LATS1 in the formation, maintenance and degeneration of corpus luteum in yak (Bos grunniens) and the effect of HCG on its expression[J]. Acta Agronomica Sinica, 2024, 32 (8): 1844- 1856.
15	DELFORGE J P , THOMAS K , ROUX F C C O , et al. Time relationships between granulosa cells growth and luteinization, and plasma luteinizing hormone discharge in human. 1. A morphometric analysis[J]. Fertil Steril, 1972, 23 (1): 1- 11.
16	ALRABIAH N A , EVANS A C , FAHEY A G , et al. Immunological aspects of ovarian follicle ovulation and corpus luteum formation in cattle[J]. Reproduction, 2021, 162 (3): 209- 225.
17	WILTBANK M C , MONTEIRO P L , DOMINGUES R R , et al. Maintenance of the ruminant corpus luteum during pregnancy: interferon-tau and beyond[J]. Animal, 2023, 17, 100827.
18	FERRARA N , DAVIS-SMYTH T . The biology of vascular endothelial growth factor[J]. Endocr Rev, 1997, 18 (1): 4- 25.
19	BERISHA B , SCHAMS D , KOSMANN M , et al. Expression and tissue concentration of vascular endothelial growth factor, its receptors, and localization in the bovine corpus luteum during estrous cycle and pregnancy[J]. Biol Reprod, 2000, 63 (4): 1106- 1114.
20	REYNOLDS L P , GRAZUL-BILSKA A T , REDMER D A . Angiogenesis in the corpus luteum[J]. Endocrine, 2000, 12, 1- 9.
21	GALAL S M , IBRAHIM S , MAHMOUD K , et al. Expression pattern of apoptotic genes during different stages of corpus luteum development in egyptian buffaloes[J]. Adv Anim Vet Sci, 2022, 10 (11): 2431- 2437.
22	P E REZ-GUTI E RREZ L , FERRARA N . Biology and therapeutic targeting of vascular endothelial growth factor A[J]. Nat Rev Mol Cell Biol, 2023, 24 (11): 816- 834.
23	张正红, 刘召远, 张经伟, 等. 家畜黄体新生血管形成的分子机制及其意义[J]. 家畜生态学报, 2020, 41 (3): 81- 85.
	ZHANG Z H , LIU Z Y , ZHANG J W , et al. Molecular mechanism and significance of new blood vessel formation in livestock corpus luteum[J]. Journal of Livestock Ecology, 2020, 41 (3): 81- 85.
24	MANN G E . Corpus luteum size and plasma progesterone concentration in cows[J]. Anim Reprod Sci, 2009, 115 (1-4): 296- 299.
25	SHARAWY H A , HEGAB A O , ELMETWALLY M A . Impact of the corpus luteum and follicular parameters on the levels of plasma progesterone and the prediction of pregnancy in Holstein dairy cows[J]. Reprod Domest Anim, 2023, 58 (11): 1525- 1531.
26	HERZOG K , BROCKHAN-L U DEMANN M , KASKE M , et al. Luteal blood flow is a more appropriate indicator for luteal function during the bovine estrous cycle than luteal size[J]. Theriogenology, 2010, 73 (5): 691- 697.
27	KANAZAWA T , SEKI M , ISHIYAMA K , et al. Pregnancy prediction on the day of embryo transfer (Day 7) and Day 14 by measuring luteal blood flow in dairy cows[J]. Theriogenology, 2016, 86 (6): 1436- 1444.
28	茆达干, 郭南南, 邝美倩. PGF₂α诱导的早期反应基因参与黄体退化的研究进展[J]. 生物技术通报, 2016, 32 (2): 30- 37.
	MA D G , GUO N N , KUANG M Q . Progress in the study of early response genes induced by PGF2α involved in luteolysis[J]. Bulletin of Biotechnology, 2016, 32 (2): 30- 37.
29	PIROKAD W , YADMAK C , YAMA P , et al. Increased luteal tissues after secondary corpus luteum formation leads to enhanced progesterone concentrations and improved fertility in repeat-breeder dairy cows during heat stress condition in tropical climate[J]. Trop Anim Health Prod, 2022, 54 (5): 308.
30	HANSEN T R , SINEDINO L D , SPENCER T E . Paracrine and endocrine actions of interferon tau (IFNT)[J]. Reproduction, 2017, 154 (5): F45- F59.
31	PROIETTO S I A , CORTASA S A E S , SCHMIDT A R U L , et al. Estradiol affects the expression of essential molecular factors involved in luteinizing hormone secretion in the plains vizcacha[J]. Gen Comp Endocrinol, 2025, 360, 114642.
32	MEZERA M A , HAMM C S , GAMARRA C A , et al. Profiles of prostaglandin F2α metabolite in dairy cattle during luteal regression and pregnancy: implications for corpus luteum maintenance[J]. Biol Reprod, 2019, 101 (1): 76- 90.
33	BORROTO-ESCUELA D O , CUESTA-MARTI C , LOPEZ-SALAS A , et al. The oxytocin receptor represents a key hub in the GPCR heteroreceptor network: potential relevance for brain and behavior[J]. Front Mol Neurosci, 2022, 15, 1055344.
34	ADHIKARI B , LEE C N , KHADKA V S , et al. RNA-Sequencing based analysis of bovine endometrium during the maternal recognition of pregnancy[J]. BMC Genomics, 2022, 23 (1): 494.
35	THATCHER W W , MEYER M D , DANET-DESNOYERS G . Maternal recognition of pregnancy[J]. J Reprod Fer Suppl Only, 1995, 49, 15- 28.
36	PENNY L A , ARMSTRONG D , BRAMLEY T A , et al. Immune cells and cytokine production in the bovine corpus luteum throughout the oestrous cycle and after induced luteolysis[J]. Reproduction, 1999, 115 (1): 87- 96.
37	IRMLER M , THOME M , HAHNE M , et al. Inhibition of death receptor signals by cellular FLIP[J]. Nature, 1997, 388 (6638): 190- 195.
38	MONTEIRO P L , WILTBANK M C , FRIZZARINI W S , et al. Hormonal profiles and biomarkers leading to parturition in cattle[J]. Biol Reprod, 2024, 111 (6): 1282- 1296.
39	STEVENSON J S . Spatial relationships of ovarian follicles and luteal structures in dairy cows subjected to ovulation synchronization: Progesterone and risks for luteolysis, ovulation, and pregnancy[J]. J Dairy Sci, 2019, 102 (6): 5686- 5698.
40	赵建发. 卵巢疾病引发奶牛不孕症的预防与治疗[J]. 中国乳业, 2024 (4): 42- 46.
	ZHAO J F . Prevention and treatment of infertility caused by ovarian diseases in dairy cows[J]. China Dairy Industry, 2024 (4): 42- 46.
41	STR U VE K , HERZOG K , MAGATA F , et al. The effect of metritis on luteal function in dairy cows[J]. BMC Vet Res, 2013, 9, 1- 9.
42	POLLOTT G E , COFFEY M P . The effect of genetic merit and production system on dairy cow fertility, measured using progesterone profiles and on-farm recording[J]. J Dairy Sci, 2008, 91 (9): 3649- 3660.
43	MOGOLL O N H , FERRAZZA R A , VALLEJO V H , et al. Heat stress does not affect induced luteolysis in Holstein cows[J]. J Dairy Sci, 2020, 103 (6): 5629- 5633.
44	HENDERSON K M , SCARAMUZZI R J , BAIRD D T . Simultaneous infusion of prostaglandin E2 antagonizes the luteolytic action of prostaglandin F2α in vivo[J]. J Endocrinol, 1977, 72 (3): 379- 383.
45	SOUZA A H , AYRES H , FERREIRA R M , et al. A new presynchronization system (Double-Ovsynch) increases fertility at first postpartum timed AI in lactating dairy cows[J]. Theriogenology, 2008, 70 (2): 208- 215.
46	GIORDANO J O , WILTBANK M C , FRICKE P M , et al. Effect of increasing GnRH and PGF₂α dose during double-ovsynch on ovulatory response, luteal regression, and fertility of lactating dairy cows[J]. Theriogenology, 2013, 80 (7): 773- 783.
47	MINELA T , GIBB P , MCBETH S , et al. Reduced period from follicular wave emergence to luteolysis generated greater steroidogenic follicles and estrus intensity in dairy cows[J]. Sci Rep, 2023, 13 (1): 22818.
48	LONERGAN P . Influence of progesterone on oocyte quality and embryo development in cows[J]. Theriogenology, 2011, 76 (9): 1594- 1601.
49	NASCIMENTO A B , BENDER R W , SOUZA A H , et al. Effect of treatment with human chorionic gonadotropin on day 5 after timed artificial insemination on fertility of lactating dairy cows[J]. J Dairy Sci, 2013, 96 (5): 2873- 2882.
50	YAN L , ROBINSON R , SHI Z , et al. Efficacy of progesterone supplementation during early pregnancy in cows: A meta-analysis[J]. Theriogenology, 2016, 85 (8): 1390- 1398.
51	LONERGAN P , S A NCHEZ J E M I , MATHEW D J , et al. Embryo development in cattle and interactions with the reproductive tract[J]. Reprod, Fert Dev, 2019, 31 (1): 118- 125.
52	CHEN F , HOU Y A , ZHU X , et al. Impact of accessory corpus luteum induced by gonadotropin-releasing hormone or human chorionic gonadotropin on pregnancy rates of dairy cattle following embryo transfer: A META-analysis[J]. Vet Sci, 2023, 10 (5): 309.
53	MONTEIRO P L , SARTORI R , CANAVESSI A M , et al. Accessory corpus luteum regression during pregnancy Ⅰ: timing, physiology, and P4 profiles[J]. Reproduction, 2021, 162 (6): 473- 482.
54	MEZERA M A , LI W , WILTBANK M C . Pregnancy-induced changes in the transcriptome of the bovine corpus luteum during and after embryonic interferon-tau secretion[J]. Biol Reprod, 2021, 105 (1): 148- 163.
55	WILTBANK M C , MEZERA M A , TOLEDO M Z , et al. Physiological mechanisms involved in maintaining the corpus luteum during the first two months of pregnancy[J]. Anim Reprod, 2018, 15 (Suppl 1): 805.
56	BETTERIDGE K J , RANDALL G , EAGLESOME M D , et al. The influence of pregnancy on PGF₂α secretion in cattle. Ⅰ. Concentrations of 15-keto-13, 14-dihydroprostaglandin F2α and progesterone in peripheral blood of recipients of transferred embryos[J]. Anim Reprod Sci, 1984, 7 (1-3): 195- 216.
57	李宇, 段春辉, 宋志攀, 等. 黄体期注射PGF₂α对母羊血清生殖激素及相关细胞因子的影响[J]. 畜牧兽医学报, 2022, 53 (6): 1807- 1818.
	LI Y , DUAN C H , SONG Z P , et al. Effects of PGF2α on reproductive hormones and related cytokines during luteal phase in ewes[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53 (6): 1807- 1818.
58	ABDO M , HISHEH S , ARFUSO F , et al. The expression of tumor necrosis factor-alpha, its receptors and steroidogenic acute regulatory protein during corpus luteum regression[J]. Reprod Biol Endocrinol, 2008, 6, 1- 11.
59	MONACO C F , DAVIS J S . Mechanisms of angioregression of the corpus luteum[J]. Front Physiol, 2023, 14, 1254943.
60	JONCZYK A W , PIOTROWSKA-TOMALA K K , SKARZYNSKI D J . Effects of prostaglandin F₂α (PGF₂α) on cell-death pathways in the bovine corpus luteum (CL)[J]. BMC Vet Res, 2019, 15, 1- 16.

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Progress in the Study of the Formation and Lysis of Corpus Luteum in Dairy Cows and Its Role in the Maintenance of Pregnancy

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