不同年龄牦牛肺脏中JAK2、STAT3、P-JAK2/STAT3及PCNA蛋白的表达分析

doi:10.11843/j.issn.0366-6964.2025.06.038

Abstract

Abstract:

This study aimed to investigate the expression distribution and possible roles of the JAK2/STAT3 signaling pathway and its regulated PCNA, a key protein for cell proliferation, in the lungs of yaks of different ages. In this experiment, the lungs of newborn, juvenile, adult and aged healthy yaks were used to detect the expression of JAK2, STAT3 and PCNA mRNA in the lungs of yaks of different ages by quantitative real-time fluorescence PCR (qRT-PCR), and the expression of JAK2, STAT3, P-JAK2/STAT3 and PCNA proteins in the lungs of yaks of different ages was detected by Immunohistochemistry (IHC) and Western blot methods. The expression distribution of JAK2, STAT3, P-JAK2/STAT3 and PCNA were basically consistent, these are mainly distributed in the bronchial epithelium and vascular endothelium, the smooth muscle layer of the bronchial wall, vascular smooth muscle and alveolar cells. The expression of JAK2 mRNA in the lungs of yaks at different ages demonstrated a tendency of increasing and then decreasing, and the expression of JAK2 mRNA in the adult group was the highest. The expression of STAT3 mRNA in the lungs increased with the age of yaks, and the expression was highest in the old group. The expression of PCNA mRNA in the adult and old groups was significantly higher than that in the newborn and juvenile groups. Additionally, the expressions of JAK2, STAT3, P-JAK2/STAT3 and PCNA proteins were different in the lungs of yaks at different ages. The expression trend of P-JAK2/STAT3 was found to be essentially identical to that of JAK2/STAT3. The expression of JAK2/P-JAK2 proteins was the highest in the juvenile group, and the expression of STAT3/P-STAT3 proteins in the newborn and adult groups was significantly higher than that in the juvenile and old groups, and the newborn group was higher than that in the adult group. The expression of the PCNA protein in the newborn group was significantly higher than that in the juvenile, adult, and old groups. The findings of this study indicated that there were differences in the expression and distribution of JAK2/STAT3 signaling pathway and its regulated PCNA in yak lungs of different ages, suggesting that the JAK2/STAT3 signaling pathway plays a role in the development of yak lungs. This study provides a research foundation for further clarifying the role of the JAK2/STAT3 signaling pathway and its targeted regulation of PCNA in the adaptation of yak lungs to the plateau environment.

Key words: yak, lung, JAK2/STAT3 signaling pathway, hypoxic pulmonary hypertension

CLC Number:

S852.1

NIU Yueyue, CUI Yan, YU Sijiu, HE Junfeng, YANG Shanshan, QI Zhengman, DOU Wanwan, CHEN Chunyan, DENG Yanjiang. Analysis of JAK2, STAT3, P-JAK2/STAT3 and PCNA Proteins Expression in the Lungs of Yaks of Different Ages[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(6): 2957-2967.

Figures/Tables 9

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基因名称Gene name	引物序列(5′→3′) Primer sequences	退火温度/℃ Tm	产物大小/bp Product size	GenBank登录号 GenBank accession number
JAK2	F: TATGGAGTATGTGTCTGTGGAGAGGAG R: TTCCTGTCTTCCTGTCTTCTTCTCTGA	61.0	235	XM_024996127.2
STAT3	F: GAGAAGGACATCAGCGGCAAGAC R: GATAGACCAGTGGAGACACCAGGAT	60.0	145	NM_001012671.2
PCNA	F: CTCGTCTCATGTCTCCTTGGT R: TGTCTTCATTGCCAGCACATT	56.4	134	XM_005906528.2
β-actin	F: CCGTGACATCAAGGACAAG R: AGGAAGGAAGGATGGAAG	60.0	174	NM_173979.3

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Analysis of JAK2, STAT3, P-JAK2/STAT3 and PCNA Proteins Expression in the Lungs of Yaks of Different Ages

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