复方羟氯扎胺混悬液对牦牛肝片吸虫和线虫的临床药效试验

doi:10.11843/j.issn.0366-6964.2024.11.041

Abstract

Abstract:

The purpose of the study was to evaluate the clinical efficacy of the compound oxyclozanide suspension through the deworming testing, and to provide reference basis for its clinical use. Seventy-five Qinghai Huanhu yaks were divided into 5 groups randomly, with 15 yaks in each group, which were compound oxyclozanide suspension low dose (0.125 mL·kg^-1), medium dose (0.250 mL·kg^-1), high dose (0.500 mL·kg^-1), control group (0.250 mL·kg^-1) and blank control group. The test cycle was 28 days. The feces were collected on the first day and on days 7, 14, 21, and 28 after administration, and the egg counts (eggs per gram, EPG) of Fasciola hepatica and digestive tract nematodes is measured, and the precision of deworming and the rate of egg conversion were calculated. The blood routine, blood biochemical indicators value were measured before and on day 28 of the administration. The precision deworming rates of low, medium, and high doses of compound oxyclozanide suspension against yak Fasciola hepatica were 97.5%, 100.0%, and 100.0% respectively, and the egg negative conversion rates were 80%, 100% and 100% respectively. The recision deworming rate of the imported medicine against yak Fasciola hepatica was 99.4%, and the egg negative conversion rate was 93.3%. The precision deworming rate of low, medium and high doses of compound oxyclozanide suspension against yak digestive tract nematodes was 99.2%, 100.0% and 100.0%, and the egg transfer rate was 80%, 100% and 100%, respectively, while the precision deworming rate of control imported drug against yak digestive tract nematodes was 99.9%, and the egg transfer rate was 93.3%.The results of this yak test showed that the compound oxyclozanide suspension had a significant treatment effect on the Fasciola hepatica insects and digestive tract nematodes. There is no significant difference in the clinical effects between the high and medium dose groups. Therefore, the recommended dose for clinical application was 0.250 mL·kg^-1 in the medium dose group.

Key words: compound oxyclozanide suspension, yak, Fasciola hepatica, digestive tract nematodes, pharmacodynamics

CLC Number:

S859.795

Juan WANG, Xueqiang YAO, Linlin LU, Jiang LI, Kairen ZHOU, Bintao ZHAI, Xuzheng ZHOU, Jiyu ZHANG. Clinical Efficacy Test of Compound Oxyclozanide Suspension on Fasciola hepatica and Nematodes of Yak[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(11): 5259-5266.

Figures/Tables 10

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References 14

1	黄纯, 阎萍, 梁春年. 中国牦牛种业现状与发展方向[J]. 中国畜禽种业, 2023, 19 (7): 121- 127.
	HUANG C , YAN P , LIANG C N . The current status and development direction of the yak industry in China[J]. The Chinese Livestock and Poultry Breeding, 2023, 19 (7): 121- 127.
2	廖党金, 李江凌. 寄生虫病对畜牧业的危害与对策[J]. 中国兽医寄生虫病, 2008, 16 (5): 59- 61.
	LIAO D J , LI J L . Parasitic diseases to the harm and countermeasures of animal husbandry[J]. Chinese Journal of Veterinary Parasitology, 2008, 16 (5): 59- 61.
3	张吉丽, 司鸿飞, 李冰, 等. 五氯柳胺的研究进展[J]. 中国兽药杂志, 2015, 49 (11): 65- 69.
	ZHANG J L , SI H F , LI B , et al. Progress in research of oxyclozanide[J]. Chinese Journal of Veterinary Drug, 2015, 49 (11): 65- 69.
4	陈玲玲, 申涵露, 张东辉, 等. 羟氯扎胺与左旋咪唑联合用药的研究进展[J]. 中国兽医科学, 2022, 52 (3): 372- 379.
	CHEN L L , SHEN H L , ZHANG D H , et al. Research progress of oxyclozanide combined with levamisole[J]. Chinese Veterinary Science, 2022, 52 (3): 372- 379.
5	GEORGIEV B , GRUEV A . Effectiveness of levamisole and oxyclozanide in paramphistomiasis in sheep and cattle[J]. Vet Med Nauki, 1979, 16 (3): 45- 51.
6	MEHMOOD K , IJAZ M , DURRANI A Z , et al. Infection rate and therapeutic trials on various gastrointestinal parasites in sheep and goats in and around Lahore, Pakistan[J]. Pakistan J Zool, 2013, 45 (2): 489- 494.
7	ROLFE P F , BORAY J C . Chemotherapy of paramphistomosis in cattle[J]. Aust Vet J, 1987, 64 (11): 328- 332. doi: 10.1111/j.1751-0813.1987.tb06060.x
8	COLES G C , STAFFORD K A . Activity of oxyclozzanide, nitroxynil, clorsulon and albendazole against adult triclabendazole resistant Fasciola hepatica[J]. Vet Rec, 2001, 148 (23): 723- 724. doi: 10.1136/vr.148.23.723
9	中华人民共和国农业部. 兽药临床试验质量管理规范(公告第2337号)[S/OL]. http://www.ivdc.org.cn/pszx/ywgz/zcfg/202108/t20210817_53401.htm.
	Ministry of Agriculture of the People's Republic of China. Veterinary drug clinical trial quality management standards (Announcement No. 2337)[S/OL]. http://www.ivdc.org.cn/pszx/ywgz/zcfg/202108/t20210817_53401.htm. (in Chinese)
10	农业部兽药评审中心. 兽药研究技术指导原则汇编(2006—2011)[M]. 北京: 化学工业出版, 2012: 192- 196.
	Ministry of Agriculture of the People's Republic of China Under Review . Compilation of technical guidelines for veterinary drug research (2006—2011)[M]. Beijing: Chemical Industry Press, 2012: 192- 196.
11	索勋, 杨晓野. 高级寄生虫学实验指导[M]. 北京: 中国农业科学技术出版社, 2005: 5- 6.
	SUO X , YANG X Y . Advanced parasitology experimental guidance[M]. Beijing: China Agricultural Science and Technology Press, 2005: 5- 6.
12	王文龙, 赵学亮, 刑海龙, 等. 伊维菌素浇泼剂和注射剂驱除牛消化道线虫临床效果观察[J]. 中国兽医杂志, 2018, 54 (1): 98- 100.
	WANG W L , ZHAO X L , XING H L , et al. Clinical observation on the efficacy of ivermectin injection and pour-on expelling digestive tract nema in cattle[J]. Chinese Journal of Veterinary Medicine, 2015, 54 (1): 98- 100.
13	SHRIMALI R G , PATEL M D , PATEL R M . Comparative efficacy of anthelmintics and their effects on hemato-biochemical changes in fasciolosis of goats of South Gujarat[J]. Vet World, 2016, 9 (5): 524- 529. doi: 10.14202/vetworld.2016.524-529
14	BYARUHANGA C , OKWEE-ACAI J . Efficacy of albendazole, levamisole and ivermectin against gastro-intestinal nematodes in naturally infected goats at the National Semi-arid Resources Research Institute, Serere, Uganda[J]. Vet Parasitol, 2013, 195 (1-2): 183- 186. doi: 10.1016/j.vetpar.2013.01.007

组别 Group	时间 Time	指标Index
组别 Group	时间 Time	WBC	EOS	RBC	HGB	HCT	MCV	MCH	MCHc	RDW	MPV
1	Day 0	7.32±2.61	0.57±0.80	6.23±0.74	9.24±1.08	30.43±3.39	48.87±3.18	14.84±1.11	30.38±0.91	20.02±0.98	6.48±0.79
	Day 28	9.26±3.00	1.05±0.98	6.39±1.15	9.45±1.66	30.89±4.85	48.8±4.02	14.85±1.25	30.51±0.88	20.83±1.53	6.44±0.49
2	Day 0	8.10±1.96	0.49±0.68	6.89±1.80	9.22±1.36	28.08±3.88	42.93±8.22	13.33±2.58	31.71±1.81	21.76±2.48	6.13±0.53
	Day 28	8.26±3.28	0.19±0.08	5.85±0.58	8.92±1.35	28.58±3.88	48.6±2.61	15.09±0.70	31.13±1.03	19.94±1.07	6.14±0.53
3	Day 0	6.97±2.28	0.25±0.12	5.85±0.58	8.73±0.83	28.22±2.05	48.4±3.04	14.99±1.44	30.93±1.98	19.76±0.95	9.53±11.77
	Day 28	7.26±1.73	0.22±0.10	9.35±1.29	9.35±1.29	30.89±4.41	47.27±2.84	14.35±1.04	30.35±1.63	19.82±0.77	6.78±0.38
4	Day 0	7.20±1.75	0.36±0.41	6.06±1.03	9.10±1.48	29.87±4.53	49.53±4.45	15.09±1.07	30.49±1.14	31.29±44.74	6.56±0.66
	Day 28	8.01±3.47	0.41±0.47	6.11±1.15	8.64±1.41	27.98±3.93	46.80±7.27	14.41±2.16	30.88±1.30	21.29±4.34	6.25±0.85
5	Day 0	6.62±1.42	0.24±0.11	6.20±0.98	9.17±1.19	29.57±4.12	47.80±3.34	14.92±1.68	31.13±2.37	19.82±0.87	6.70±0.67
	Day 28	6.82±1.58	0.23±0.10	6.85±1.20	9.64±1.39	32.14±5.09	46.86±3.21	14.13±1.21	30.17±2.08	20.01±0.67	6.52±0.32

组别 Group	时间 Time	指标Index
组别 Group	时间 Time	TP	ALB	ALT	AST	ALP	GGT	LDH
1	Day 0	66.58±5.44	20.41±2.33	36.50±7.34	79.20±14.50	105.20±27.70	12.40±5.73	790.00±137.67
	Day 28	66.65±6.01	20.75±2.34	34.60±6.39	80.27±12.93	152.87±82.76	18.31±16.13	857.27±205.79
2	Day 0	62.33±8.30	20.80±2.74	32.38±6.21	81.13±15.07	147.20±61.50	18.84±17.04	865.67±204.25
	Day 28	67.42±7.26	22.19±1.86	29.69±6.37	82.86±19.05	182.29±79.70	19.71±9.97	859.57±179.15
3	Day 0	69.72±3.27	22.23±2.14	33.46±8.30	81.27±17.407	132.47±49.98	17.09±17.02	872.33±228.37
	Day 28	68.37±7.46	22.37±2.27	30.72±7.23	76.07±11.72	111.53±36.01	13.57±9.68	828.67±231.38
4	Day 0	66.37±7.61	21.19±2.89	31.64±4.12	75.20±11.14	202.60±112.93	20.27±12.20	807.27±118.85
	Day 28	67.94±10.32	24.06±1.96	25.47±15.11	96.27±16.99	287.80±131.46	36.55±22.17	587.67±219.37
5	Day 0	68.86±5.82	24.00±1.60	36.84±9.43	84.27±12.81	237.00±71.47	18.53±4.16	766.33±116.84
	Day 28	72.89±4.42	24.09±2.70	37.02±6.49	86.93±14.43	175.13±85.07	22.03±3.02	824.07±129.43

组别Group	Day 0	Day 7	Day 14	Day 21	Day 28
1	130.8±40.9^a	7.5±14.0^Bb	3.3±7.4^Bb	9.16±23.8^Bb	36.7±33.2^Bb
2	145.8±29.8^a	0±0^Bb	0±0^Bb	1.6±4.3^Bb	3.3±5.7^Bb
3	145.8±33.6^a	0±0^Bb	0±0^Bb	0.8±3.2^Bb	3.3±7.4^Bb
4	147.5±30.7^a	4.2±9.0^Bb	0.8±3.2^Bb	3.3±5.7^Bb	5.8±7.9^Bb
5	140.0±36.0^a	152.5±35.1^Aa	170±32.3^Aa	145.8±33.9^Aa	141.7±38.9^Aa

给药后时间 Time	组别Group
给药后时间 Time	1	2	3	4	5
Day 7	94.3	100.0	100.0	97.1	0.0
Day 14	97.5	100.0	100.0	99.4	0.0
Day 21	92.9	98.8	99.4	97.7	0.0
Day 28	71.9	97.7	97.7	96.0	0.0

给药后时间 Time	组别Group
给药后时间 Time	1	2	3	4	5
Day 7	73.3	100.0	100.0	80.0	0.0
Day 14	80.0	100.0	100.0	93.3	0.0
Day 21	80.0	86.6	93.3	73.3	0.0
Day 28	53.3	73.3	80.0	60.0	0.0

Clinical Efficacy Test of Compound Oxyclozanide Suspension on Fasciola hepatica and Nematodes of Yak

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组别 Group	Day 0	Day 7	Day 14	Day 21	Day 28
1	513.40±121.90^a	44.30±31.50^Bb	3.60±7.80^Bb	31.30±33.90^Bb	37.20±31.90^Bb
2	525.50±120.80^a	6.90±12.10^Bb	0±0^Bb	12.80±15.40^Bb	25.10±21.90^Bb
3	502.63 ±110.93^a	6.10±11.10^Bb	0±0^Bb	9.10±12.50^Bb	23.40±22.90^Bb
4	516.70±129.40^a	5.10±7.60^Bb	0.50±1.80^Bb	10.40±15.10^Bb	32.00±24.10^Bb
5	444.23±108.52^b	562.33±84.97^Aa	598.47±91.85^Aa	521.00±72.97^Aab	452.00±90.05^Ab