阿司匹林丁香酚酯对蛋鸡脂肪肝出血综合征的预防作用

doi:10.11843/j.issn.0366-6964.2025.06.039

Abstract

Abstract:

This study aimed to investigate the preventive effects of aspirin eugenol ester (AEE) on fatty liver hemorrhagic syndrome (FLHS) in laying hens. A total of seventy-eight 160-day-old Hy-Line Brown laying hens were randomly assigned to six groups, each containing 13 hens. Results were as follows: Group Ⅰ received a basal diet, while Groups Ⅱ to Ⅴ were fed a high-energy, low-protein diet supplemented with 0, 25, 50, and 100 mg·kg^-1 of AEE, respectively. Group Ⅵ was provided a diet containing 2 g·kg^-1 choline chloride. The experiment lasted for 90 days. The results are in the following fashion: ① Compared to Group Ⅰ, Group Ⅱ showed a significant decrease in egg production rate and average egg weight (P < 0.05), and a significant increase in the feed-to-egg ratio (P < 0.05). In comparison to Group Ⅱ, Groups Ⅲ to Ⅵ exhibited a significant increase in egg production rate and average egg weight at different time points (P < 0.05), and a significant decrease in the feed-to-egg ratio (P < 0.05). ② Compared to Group Ⅰ, the body weight of hens in Group Ⅱ significantly increased (P < 0.05). However, compared to Group Ⅱ, the body weights of hens in Groups Ⅲ to Ⅵ significantly decreased (P < 0.05). ③ Compared to Group Ⅰ, the hens in Group Ⅱ showed significantly enlarged livers with a yellowish color, punctate hemorrhages on the surface, and increased friability. HE staining revealed disordered hepatocyte arrangement and the presence of fat vacuoles of various sizes. In contrast, the liver structure of hens in Groups Ⅲ to Ⅵ returned to normal compared to Group Ⅱ. ④ Liver Index and Lipid Levels: Compared to Group Ⅰ, Group Ⅱ showed significantly increased liver index and elevated levels of triglyceride (TG), total cholesterol (TC), and low-density lipoprotein (LDL) in serum and liver tissues, with significantly decreased high-density lipoprotein (HDL) levels (P < 0.05). However, compared to Group Ⅱ, Groups Ⅲ to Ⅵ exhibited significantly lower liver index, TG, TC, and LDL levels, and significantly increased HDL levels (P < 0.05). ⑤ Group Ⅱ exhibited significantly lower activities of liver superoxide dismutase (SOD), glutathione peroxidase (GSH), and catalase (CAT), along with significantly higher malondialdehyde (MDA) levels compared to Group Ⅰ (P < 0.05). In comparison, Groups Ⅲ to Ⅵ had significantly higher SOD, GSH, and CAT activities and lower MDA levels compared to Group Ⅱ (P < 0.05). The results indicate that feeding laying hens a high-energy, low-protein diet effectively induces fatty liver hemorrhagic syndrome. Supplementing this diet with varying doses of AEE demonstrates a protective effect against FLHS in laying hens.

Key words: aspirin eugenol ester, laying hens, fatty liver hemorrhagic syndrome, antioxidant markers

CLC Number:

S859.7

YAN Kai, XU Xiao, QING Zhe, BAI Lixia, LI Zhun, YANG Yajun, LIU Xiwang, LI Shihong, GE Wenbo, LI Jianyong, LI Cun. Preventive Effect of Aspirin Eugenol Ester on Fatty Liver Hemorrhagic Syndrome in Laying Hens[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(6): 2968-2977.

Figures/Tables 8

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References 30

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项目Items	含量Content
项目Items	基础日粮 Basic diet	高能低蛋白日粮 High-energy low-protein diet
原料Ingredients
玉米Corn	64.00	70.00
豆粕Soybean meal	26.00	15.78
豆油Soybean oil		4.22
钙Calcium	8.00	8.00
预混料Premix^*	2.00	2.00
合计Total	100.00	100.00
营养水平Nutrient level
代谢能ME/(kJ·kg^-1)	11 248.64	13 212.22
粗蛋白质CP	15.50	12.30
有效磷AP	0.53	0.51
精氨酸Arg	1.03	0.74
蛋氨酸Met	0.37	0.32
缬氨酸Val	0.77	0.58
蛋氨酸+半胱氨酸Met+Cys	0.67	0.56

时间/d Time	项目Item	组别Groups
时间/d Time	项目Item	Ⅰ	Ⅱ	Ⅲ	Ⅳ	Ⅴ	Ⅵ
1~30	产蛋率/% Reproductive performance	91.92±7.25^a	85.94±9.39^b	88.75±4.42^ab	90.61±7.12^ab	92.31±7.77^a	90.49±8.70^ab
	料蛋比 Feed conversion ratio	2.20±0.55^ab	2.42±0.48^a	2.16±0.28^b	2.21±0.34^ab	2.06±0.33^b	2.28±0.55^ab
	蛋重/g Egg weight	56.47±1.42^a	54.83±1.92^c	55.11±1.92^bc	54.55±2.13^c	55.91±2.24^ab	54.60±1.72^c
31~60	产蛋率/% Reproductive performance	90.59±7.01^a	74.64±13.53^b	85.98±8.51^a	84.38±13.01^a	88.85±9.19^a	84.31±9.98^a
	料蛋比 Feed conversion ratio	2.22±0.45^b	2.61±0.90^a	2.21±0.55^b	2.36±0.47^ab	2.14±0.35^b	2.24±0.34^b
	蛋重/g Egg weight	56.74±1.68^ab	54.44±2.08^c	57.20±0.83^a	56.41±1.08^ab	56.70±0.96^ab	55.10±1.5^bc
61~90	产蛋率/% Reproductive performance	90.28±6.63^a	77.56±10.21^c	82.81±8.84^bc	85.83±11.18^ab	86.90±11.95^ab	81.28±8.44^bc
	料蛋比 Feed conversion ratio	2.24±0.31^bc	2.69±0.44^a	2.39±0.45^b	2.39±0.62^b	2.11±0.26^c	2.40±0.37^b
	蛋重/g Egg weight	59.52±1.54^a	57.78±2.18^b	58.13±2.01^b	58.83±1.67^ab	58.97±1.60^ab	58.19±2.09^b

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Preventive Effect of Aspirin Eugenol Ester on Fatty Liver Hemorrhagic Syndrome in Laying Hens

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