Acta Veterinaria et Zootechnica Sinica ›› 2025, Vol. 56 ›› Issue (6): 2948-2956.doi: 10.11843/j.issn.0366-6964.2025.06.037
• Basic Veterinary Medicine • Previous Articles Next Articles
ZHENG Yilei1,2(), SHEN Fengting1,2(
), XIN Liang1, YUAN Xiwang1, LIN Lin3, CAO Di3, WU Jiusheng1,2, SHI Fushan1,2, WANG Huanan1,2,*(
)
Received:
2024-07-23
Online:
2025-06-23
Published:
2025-06-25
Contact:
WANG Huanan
E-mail:yileizheng@zju.edu.cn;22317101@zju.edu.cn;hnwang@zju.edu.cn
CLC Number:
ZHENG Yilei, SHEN Fengting, XIN Liang, YUAN Xiwang, LIN Lin, CAO Di, WU Jiusheng, SHI Fushan, WANG Huanan. Pathological and Immunohistochemical Analysis of A Case of Feline Nasal Malignant Neuroendocrine Neoplasm[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(6): 2948-2956.
Table 1
Abnormal results of laboratory tests"
项目 Project | 结果 Result | 参考值 Reference value | 单位 Unit |
红细胞分布宽度 Red distribution width (RDW) | 29.4↑ | 15.0~27.0 | % |
血小板Platelet (PLT) | 0.23↓ | 1.51~6.00 | 105·μL-1 |
降钙素原Procalcitonin (PCT) | 0.04↓ | 0.17~0.86 | % |
活化部分凝血酶时间 Activated partial thromboplastin time (aPTT) | 25.0↓ | 60.0~115.0 | s |
Fig. 1
Cytologic results of Diff-Quick staining of nasal mass A. In the center of the field of view, there was an accumulation of vesicular naked nuclei with a large number of light blue lymphoid bodies (arrow); B. Microscopically, the tumor cells are nested and the tumor cells are ovoid (arrow)"
Fig. 3
Tumor pathology examination results (HE staining) A. Tumor and surrounding tissues, the tumor is located in the submucosal lamina propria, with no peripheral membrane; B, C. The tumor cell population shows infiltrative growth, with sheet-like infiltration in the dermis and muscle tissue, demonstrating marked invasiveness, 10×; D. The tumor cells display prominent nucleoli and overall moderate to high-grade atypia (arrow), 40×; E. Binucleated and multinucleated cells (arrow) can be observed, 40×; F. Vacuolar structures (arrows) are visible within the cytoplasm of tumor cells, 40×"
Fig. 4
Immunohistochemistry results (20×) A. NSE staining shows positive cytoplasmic immunoreactivity (arrow); B. CgA staining shows no immunoreactivity in tumor cells; C. Syn staining shows positive cytoplasmic immunoreactivity (arrow); D-G. CK, MAP-2, NeuN, and S-100 staining show no immunoreactivity in tumor cells; H. VIP staining shows strong positive cytoplasmic immunoreactivity (arrow); I. CD99 staining shows paranuclear positive (arrow)"
Table 2
Immunohistochemistry staining antibody information"
抗体 Antigen | 种属来源 Host species | 克隆性 Clonality | 稀释比 Dilution | 货号 Product number |
神经元特异性烯醇化酶 Neuron specific enolase (NSE) | 兔 Rabbit | 多克隆 Poly | 1:500 | GB11376-1 |
嗜铬粒蛋白A Chromorganin A (CgA) | 兔 Rabbit | 多克隆 Poly | 1:1 000 | GB111316 |
突触素 Synaptophysin (Syn) | 鼠 Mouse | 多克隆 Poly | 1:500 | GB15553 |
细胞角蛋白 Cytokeratin (CK) | 鼠 Mouse | 单克隆 Mono | 1:1 000 | GB122053 |
微管相关蛋白2 Microtubule associated protein 2 (MAP-2) | 兔 Rabbit | 多克隆 Poly | 1:2 000 | GB11128 |
神经元核 Neuronal nucleus (NeuN) | 鼠 Mouse | 单克隆 Mono | 1:500 | GB15138 |
钙结合蛋白 Soluble protein-100 (S-100) | 兔 Rabbit | 多克隆 Poly | 1:500 | GB11359 |
血管活性肠肽 Vasoactive intestinal peptide (VIP) | 兔 Rabbit | 多克隆 Poly | 1:500 | GB11279 |
CD99 Cluster of differentiation 99 (CD99) | 兔 Rabbit | 多克隆 Poly | 1:100 | GB111064 |
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