红景天苷对甲泼尼龙诱导的肉鸡股骨头坏死的影响

doi:10.11843/j.issn.0366-6964.2025.05.044

Abstract

Abstract:

This study aims to investigate the effects and mechanisms of salidroside on bone metabolism in a methylprednisolone-induced animal model of femoral head necrosis in broilers. Thirty-two 1-day-old Arbor Acres broiler chickens were randomly divided into a control group, a methylprednisolone group, a salidroside intervention group, and a salidroside control group, with 8 chickens in each group. At 42 days of age, the chickens were subjected to gait scoring and weighing, followed by the collection of blood and liver samples for triglyceride and total cholesterol analysis. Femoral head tissue samples were collected for bone metabolism marker analysis, and femur and tibia samples were taken for bone density and strength testing. Results were as follows: Compared to the methylprednisolone group, the salidroside intervention group showed a significant increase in body weight, femur weight, length, Young's modulus, and fracture load, as well as a significant increase in tibial stiffness; The transcription of Collagen-1 and Runx2 genes in the femoral head was significantly upregulated; There was a reduction in cartilage lacuna vacuolation, with significant upregulation of Collagen-2, Aggrecan, and BMP2 gene transcription in cartilage; a significant downregulation of MMP13 gene transcription; the levels of triglycerides and total cholesterol in the liver and serum significantly decreased; the expression of FASN and SCD1 genes in the femoral head was downregulated. The results indicate that salidroside can regulate lipid metabolism and cartilage matrix synthesis, and alleviate methylprednisolone induced femoral head necrosis in broiler chickens.

Key words: femoral head necrosis, salidroside, broiler, methylprednisolone, lipid metabolism

CLC Number:

ZHANG Yanyan, GE Hongfan, ZHOU Zhenlei. The Effect of Salidroside on Methylprednisolone Induced Femoral Head Necrosis in Broiler Chickens[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(5): 2496-2506.

Figures/Tables 9

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References 32

1	GUO Y P , TANG H H , WANG X N , et al. Clinical assessment of growth performance, bone morphometry, bone quality, and serum indicators in broilers affected by valgus-varus deformity[J]. Poult Sci, 2019, 98 (10): 4433- 4440. doi: 10.3382/ps/pez269
2	ZHANG M , SHI C Y , ZHOU Z L , et al. Bone characteristics, histopathology, and chondrocyte apoptosis in femoral head necrosis induced by glucocorticoid in broilers[J]. Poult Sci, 2017, 96 (6): 1609- 1614. doi: 10.3382/ps/pew466
3	KANG P D , GAO H , PEI F X , et al. Effects of an anticoagulant and a lipid-lowering agent on the prevention of steroid-induced osteonecrosis in rabbits[J]. Int J Exp Pathol, 2010, 91 (3): 235- 243. doi: 10.1111/j.1365-2613.2010.00705.x
4	CUI Q , WANG G J , SU C C , et al. The otto aufranc award. Lovastatin prevents steroid induced adipogenesis and osteonecrosis[J]. Clin Orthop Relat Res, 1997 (344): 8- 19.
5	YU Y L , WANG S J , ZHOU Z L . Cartilage homeostasis affects femoral head necrosis induced by methylprednisolone in broilers[J]. Int J Mol Sci, 2020, 21 (14): 4841. doi: 10.3390/ijms21144841
6	LIU K P , WANG K B , WANG L G , et al. Changes of lipid and bone metabolism in broilers with spontaneous femoral head necrosis[J]. Poult Sci, 2021, 100 (3): 100808. doi: 10.1016/j.psj.2020.10.062
7	FAN R B , LIU K P , ZHOU Z L . Abnormal lipid profile in fast-growing broilers with spontaneous femoral head necrosis[J]. Front Physiol, 2021, 12, 685968. doi: 10.3389/fphys.2021.685968
8	YAN Y Z , WANG J H , HUANG D G , et al. Plasma lipidomics analysis reveals altered lipids signature in patients with osteonecrosis of the femoral head[J]. Metabolomics, 2022, 18 (2): 14. doi: 10.1007/s11306-022-01872-0
9	CHANG C , GREENSPAN A , GERSHWIN M E . The pathogenesis, diagnosis and clinical manifestations of steroid-induced osteonecrosis[J]. J Autoimmun, 2020, 110, 102460. doi: 10.1016/j.jaut.2020.102460
10	PECKETT A J , WRIGHT D C , RIDDELL M C . The effects of glucocorticoids on adipose tissue lipid metabolism[J]. Metabolism, 2011, 60 (11): 1500- 1510. doi: 10.1016/j.metabol.2011.06.012
11	ROQUETA-RIVERA M , ESQUEJO R M , PHELAN P E , et al. SETDB2 links glucocorticoid to lipid metabolism through Insig2a regulation[J]. Cell Metab, 2016, 24 (3): 474- 484. doi: 10.1016/j.cmet.2016.07.025
12	WANG A , REN M , WANG J C . The pathogenesis of steroid-induced osteonecrosis of the femoral head: a systematic review of the literature[J]. Gene, 2018, 671, 103- 109. doi: 10.1016/j.gene.2018.05.091
13	TEITELBAUM S L . Bone resorption by osteoclasts[J]. Science, 2000, 289 (5484): 1504- 1508. doi: 10.1126/science.289.5484.1504
14	RECIO M C , GINER R M , MÁÑEZ S . Immunmodulatory and antiproliferative properties of Rhodiola species[J]. Planta Med, 2016, 82 (11-12): 952- 960.
15	HU M L , ZHANG D R , XU H Y , et al. Salidroside activates the AMP-activated protein kinase pathway to suppress nonalcoholic Steatohepatitis in mice[J]. Hepatology, 2021, 74 (6): 3056- 3073. doi: 10.1002/hep.32066
16	CUI Z F , JIN N N , AMEVOR F K , et al. Dietary supplementation of salidroside alleviates liver lipid metabolism disorder and inflammatory response to promote hepatocyte regeneration via PI3K/AKT/Gsk3-β pathway[J]. Poult Sci, 2022, 101 (9): 102034. doi: 10.1016/j.psj.2022.102034
17	XIE B C , ZHOU H , LIU H Y , et al. Salidroside alleviates dexamethasone-induced inhibition of bone formation via transforming growth factor-beta/Smad2/3 signaling pathway[J]. Phytother Res, 2023, 37 (5): 1938- 1950. doi: 10.1002/ptr.7711
18	ZHANG Y Y , GE H F , YU Y L , et al. Dietary salidroside supplementation improves meat quality and antioxidant capacity and regulates lipid metabolism in broilers[J]. Food Chem X, 2024, 22, 101406. doi: 10.1016/j.fochx.2024.101406
19	KESTIN S C , KNOWLES T G , TINCH A E , et al. Prevalence of leg weakness in broiler chickens and its relationship with genotype[J]. Vet Rec, 1992, 131 (9): 190- 194. doi: 10.1136/vr.131.9.190
20	YU Y L , LIN L S , LIU K P , et al. Effects of simvastatin on cartilage homeostasis in steroid-induced osteonecrosis of femoral head by inhibiting glucocorticoid receptor[J]. Cells, 2022, 11 (24): 3945. doi: 10.3390/cells11243945
21	LIU K P , FAN R B , ZHOU Z L . Endoplasmic reticulum stress, chondrocyte apoptosis and oxidative stress in cartilage of broilers affected by spontaneous femoral head necrosis[J]. Poult Sci, 2021, 100 (8): 101258. doi: 10.1016/j.psj.2021.101258
22	WOJCIECHOWSKA-PUCHAŁKA J , CALIK J , KRAWCZYK J , et al. The effect of caponization on bone homeostasis of crossbred roosters. I. Analysis of tibia bone mineralization, densitometric, osteometric, geometric and biomechanical properties[J]. Sci Rep, 2023, 13 (1): 14512. doi: 10.1038/s41598-023-41806-x
23	ZHENG H X , QI S S , CHEN C . Salidroside improves bone histomorphology and prevents bone loss in ovariectomized diabetic rats by upregulating the OPG/RANKL ratio[J]. Molecules, 2018, 23 (9): 2398. doi: 10.3390/molecules23092398
24	CHEN J J , ZHANG N F , MAO G X , et al. Salidroside stimulates osteoblast differentiation through BMP signaling pathway[J]. Food Chem Toxicol, 2013, 62, 499- 505. doi: 10.1016/j.fct.2013.09.019
25	SUN M , LU Z H , CAI P A , et al. Salidroside enhances proliferation and maintains phenotype of articular chondrocytes for autologous chondrocyte implantation (ACI) via TGF-β/Smad3 Signal[J]. Biomed Pharmacother, 2020, 122, 109388. doi: 10.1016/j.biopha.2019.109388
26	WU M Z , HU R , WANG J W , et al. Salidroside suppresses IL-1β-induced apoptosis in chondrocytes via phosphatidylinositol 3-kinases (PI3K)/Akt signaling inhibition[J]. Med Sci Monit, 2019, 25, 5833- 5840. doi: 10.12659/MSM.917851
27	JONASON J H , XIAO G , ZHANG M , et al. Post-translational regulation of Runx2 in bone and cartilage[J]. J Dent Res, 2009, 88 (8): 693- 703. doi: 10.1177/0022034509341629
28	TIAN L , YU X J . Lipid metabolism disorders and bone dysfunction--interrelated and mutually regulated (review)[J]. Mol Med Rep, 2015, 12 (1): 783- 794. doi: 10.3892/mmr.2015.3472
29	DURING A , PENEL G , HARDOUIN P . Understanding the local actions of lipids in bone physiology[J]. Prog Lipid Res, 2015, 59, 126- 146. doi: 10.1016/j.plipres.2015.06.002
30	CHEN X , WANG C Y , ZHANG K , et al. Reduced femoral bone mass in both diet-induced and genetic hyperlipidemia mice[J]. Bone, 2016, 93, 104- 112. doi: 10.1016/j.bone.2016.09.016
31	PACKIALAKSHMI B , LIYANAGE R , LAY J O Jr , et al. Prednisolone-induced predisposition to femoral head separation and the accompanying plasma protein changes in chickens[J]. Biomark Insights, 2015, 10, 1- 8.
32	PACKIALAKSHMI B , LIYANAGE R , LAY J O Jr , et al. Proteomic changes in the plasma of broiler chickens with femoral head necrosis[J]. Biomark Insights, 2016, 11, 55- 62.

FHN评分 FHN score	股骨头损伤程度 Degree of femoral head injury	是否记为 FHN
0	股骨头形态正常，未见明显损伤	否
1	股骨头关节软骨与生长板分离	是
2	股骨头破裂，骨骺骨折	是

基因Genes	引物序列(5′ →3′) Primer sequences	产物长度/bp Prodduct length	登录号 Accession No.
Collagen-1	F: GTCATTCCACCCCACGTCAT R: GTCATTCCACCCCACGTCAT	164	NM_001396622
Runx2	F: TTCACAAGCATTTCATCCCTC R: TTGCGGACATACCCAGTGACA	118	NM_204128
Collagen-2	F: ACCTACAGCGTCTTGGAGGA R: ATATCCACGCCAAACTCCTG	155	NM_204426
Aggrecan	F: TGCAAGGCAAAGTCTTCTACG R: GGCAGGGTTCAGGTAAACG	248	NM_001396162
BMP2	F: TGGTGGAGGTGGTTCACTTGGA R: TCCCTTGCCATCATGCCCAAAC	140	NM_001398170
MMP13	F: AGAGACCCTGGAGCACTGATGT R: GGGATCTCTGTCTCCAGCACCA	120	NM_001293090
FASN	F: TGCTATGCTTGCCAACAGGA R: ACTGTCCGTGACGAATTGCT	128	NM_205155
SCD1	F: GATGTCATAGAGAAGGGCCAG R: GATTCATCCCAGAAGTACCAGG	149	DQ645535
GAPDH	F: GAACATCATCCCAGCGTCCA R: CGGCAGGTCAGGTCAACAAC	132	NM_204305

指标 Index	组别Group				P值 P value
指标 Index	CON	MP	MP+SAL	SAL	P值 P value
体重/g Weight	2 383.75±124.07^a	1 881.25±131.06^c	2 106.88±103.30^b	2 300.13±116.82^a	0.000
肝脏指数/% Liver index	2.43±0.20^ab	2.67±0.23^a	2.16±0.21^b	2.19±0.20^ab	0.000
脾脏指数/% Spleen index	0.16±0.02^a	0.15±0.02^a	0.14±0.01^a	0.15±0.01^a	0.231
法氏囊指数/% Bursae index of Fabricims icius	0.18±0.02^a	0.17±0.02^a	0.16±0.02^a	0.17±0.02^a	0.336
心脏指数/% Heart index	0.49±0.04^a	0.48±0.03^a	0.45±0.03^a	0.45±0.04^a	0.055

组别(n=8) Group	FHN评分FHN score			总数 Total	发生率/% Incidence rate
组别(n=8) Group	0	1	2	总数 Total	发生率/% Incidence rate
对照组CON	12	3	1	16	25.00
甲泼尼龙处理组MP	5	7	4	16	68.75
红景天干预组MP+SAL	9	5	2	16	43.75
红景天对照组SAL	14	1	0	16	6.67

样本Samle	指标Index	CON	MP	MP+SAL	SAL	P值P Value
胫骨 Tibia	重量/g	16.09±2.89^a	11.94±2.23^b	11.69±1.29^b	16.90±2.05^a	0.01
	长度/mm	104.54±3.73^a	93.26±5.27^b	93.56±4.11^b	105.36±3.22^a	＜0.01
	直径/mm	8.68±0.66^a	7.88±0.78^a	8.13±0.57^a	9.12±0.45^a	0.075
	骨矿物质含量/g	3.07±0.43^a	2.51±0.58^a	2.69±0.26^a	3.06±0.34^a	0.054
	骨密度/(g·cm^-2)	0.21±0.01^a	0.20±0.02^a	0.21±0.01^a	0.22±0.02^a	0.923
	刚度/(N·mm^-1)	90.83±11.78^a	60.56±10.00^b	78.61±7.44^a	91.77±10.42^a	＜0.01
	杨氏模量/GPa	1.32±0.11^a	0.86±0.18^b	1.03±0.14^b	1.31±0.10^a	＜0.01
	断裂载荷/N	250.87±42.78^a	143.59±32.56^b	185.09±42.48^b	268.5±34.81^a	＜0.01
	断裂应力/MPa	50.92±5.70^a	34.85±7.30^b	40.04±6.87^b	56.13±6.44^a	＜0.01
	断裂能/J	0.74±0.14^a	0.54±0.09^b	0.61±0.12^ab	0.76±0.12^a	0.010
股骨 Femur	重量/g	11.32±1.60^a	7.40±1.50^c	9.34±1.13^b	12.22±1.41^a	＜0.01
	长度/mm	73.88±1.39^a	62.96±4.71^b	69.19±5.03^a	75.25±2.54^a	＜0.01
	直径/mm	9.45±0.92^a	8.33±0.33^b	8.59±0.71^ab	10.08±1.07^a	0.012
	骨矿物质含量/g	1.94±0.36^a	1.60±0.43^a	1.62±0.27^a	2.12±0.33^a	0.122
	骨密度/(g·cm^-2)	0.20±0.02^a	0.20±0.03^a	0.20±0.02^a	0.21±0.02^a	0.984
	刚度/(N·mm^-1)	156.61±20.21^a	129.63±20.58^b	152.90±18.35^ab	171.07±24.39^a	0.002
	杨氏模量/GPa	0.75±0.13^a	0.48±0.11^b	0.65±0.05^a	0.77±0.10^a	＜0.01
	断裂载荷/N	272.69±26.88^a	170.73±21.75^c	212.46±22.64^b	279.01±20.90^a	＜0.01
	断裂应力/MPa	39.31±4.36^a	27.05±4.44^b	31.69±3.40^b	40.63±3.90^a	＜0.01
	断裂能/J	0.42±0.11^a	0.31±0.08^a	0.32±0.08^a	0.43±0.08^a	0.051

The Effect of Salidroside on Methylprednisolone Induced Femoral Head Necrosis in Broiler Chickens

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