低温对合作猪脂肪组织形态、脂代谢相关基因表达和酶活性及AMPK/PGC-1α通路的影响

doi:10.11843/j.issn.0366-6964.2024.08.015

Abstract

Abstract:

This study aimed to investigate the effects of low temperature on adipose tissue morphology, lipid metabolism-related gene expression and enzyme activities, and AMPK/PGC-1α pathway in Hezuo pigs. Ten Hezuo pigs with similar body weight and good health condition at 75 days of age were selected and randomly divided into a control group ((23±2)℃) and a low-temperature group ((-15±2)℃), each with 5 pigs, after being reared for 7 d at room temperature, for 15 d of the experimental period. At the end of the experiment, the adipose tissue was collected from the axilla and groin of the Hezuo pigs, and the morphology of the adipose tissue was observed by HE staining; the activity of lipid metabolism-related enzymes was detected by ELISA; the expression of adipose browning heat-producing genes and lipid metabolism-related genes was detected by fluorescence quantitative PCR; and the expression of the AMPK/PGC-1α pathway proteins was detected by Western blot. The results showed that, compared with the control group, the axillary and inguinal fat of the Hezuo pigs showed browning changes, the number of adipocytes became larger, and the cross-sectional area was reduced after the low-temperature treatment; LPL activity, IL-6 mRNA expression and AMPK, P-AMPK and PGC-1α protein expression were highly significantly increased in axillary and inguinal fat (P < 0.01), and LEP mRNA expression and FAS activity were highly significantly decreased (P < 0.01); UCP3 and HSP70 mRNA expression were significantly increased in axillary fat (P < 0.05), and PPARγ mRNA expression was significantly decreased (P < 0.05), AMPK and TNF-α mRNA expression was highly significantly increased (P < 0.01); AMPK and TNF-α mRNA expression was significantly increased (P < 0.05) in inguinal fat, UCP3 and HSP70 mRNA expression was highly significantly increased (P < 0.01), PPARγ mRNA expression was highly significantly decreased (P < 0.01); PGC-1α, ADPN, FAT mRNA expression and HSL activity were significantly increased (P < 0.05) in inguinal fat but not in axillary fat (P>0.05) in Hezuo pigs after low-temperature treatment. In summary, the axillary fat and inguinal fat of Hezuo pigs under low temperatures underwent browning, and the area of adipocytes decreased, the number increased, the body heat production increased, and the key genes of lipid metabolism LEP, ADPN, PPARγ, FAT and the AMPK/PGC-1α pathway might be involved in the lipid metabolism of the organism.

Key words: low temperature, adipose tissue, lipid metabolism, AMPK/PGC-1α

CLC Number:

S828.2

Yao LI, Rui JIA, Jie LI, Shuangbao GUN, Qiaoli YANG, Longlong WANG, Pengxia ZHANG, Xiaoli GAO, Xiaoyu HUANG. Effects of Low Temperature on Adipose Tissue Morphology, Lipid Metabolism-Related Gene Expression and Enzyme Activities, and AMPK/PGC-1α Pathway in Hezuo Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3418-3426.

Figures/Tables 5

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References 35

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基因 Gene	引物序列(5′-3′) Primers sequence	产物长度/bp Product length	登录号 Accession No.
UCP3	F: TCACCTTCAGGACACGTTCG R: AGGCATCCATCCTAGTGGGT	84	NM_214049.1
PGC-1α	F: CATGTGCAACCAGGACTCTGT R: GCGTCTCTGTGAGAACTGCTA	269	NM_213963.2
HSP70	F: GCCGAGAAGGACGAGTTTGA R: TGGTACAGTCCGCTGATGATG	77	NM_213766.1
FAT	F: TGGTTTAGGAATCCCACTGCC R: GCTTCAAGTGCTGGGTCAAAA	117	NM_005667694.3
LEP	F: ACCCTCATCAAGACGATTGTCAC R: ATCACATTTCTGCAAGGCAGACT	185	XM_021078503.1
ADPN	F: GGGTCACTGTCCCTAAC R: GTCCTGGTACTGGTCGT	209	NM_214370.1
PPARγ	F: CTGACCAAAGCAAAGGCGAG R: CGGAGCGAAACTGACACCC	187	XM_005669788.3
AMPK	F: ACCAGGACCCTTTGGCAGTT R: GAATCAGGTGGGCTTGTTGC	100	XM_021076522.1
IL-6	F: CCTGAACCTTCCAAAAATGG R: ACCGGTGGTGATTCTCATC	88	NM_214399.1
TNF-α	F: GCACTGAGAGCATGATCCG R: AACCTCGAAGTGCAGTAGG	161	NM_214022.1
GAPDH	F: AGTATGATTCCACCCACGGC R: TACGTAGCACCAGCATCACC	139	NM_001206359.1

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Effects of Low Temperature on Adipose Tissue Morphology, Lipid Metabolism-Related Gene Expression and Enzyme Activities, and AMPK/PGC-1α Pathway in Hezuo Pigs

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