添加红景天苷的全价粮对犬血液生化指标和肝转录组学的影响

doi:10.11843/j.issn.0366-6964.2025.01.041

Abstract

Abstract:

The aim of this study was to investigate the effects of salidroside-added complete nutrition food on blood and liver transcriptomics in dogs, with a view to provide experimental data to support liver conservation and prevent liver disease in dogs. Ten adult Chinese field dogs (8.67±1.00 kg) were selected and randomly divided into control (n=5) and test (n=5) groups. The control group was fed with control food, while the test group was fed with complete nutrition food supplemented with salidroside. The study evaluated the feeding effect and safety of complete nutrition food supplemented with salidroside through blood examination. Additionally, the potential regulatory mechanism of salidroside-added complete nutrition food on dogs was explored using transcriptomics technology. The trial period lasted 8 weeks. The blood test results showed that salidroside-added complete nutrition food significantly reduced the concentration of serum alanine aminotransferase in dogs (P < 0.05), and did not significantly affect most of the other indexes (P>0.05). However, compared to the control food, the complete nutrition food with added salidroside did not cause greater fluctuations in blood indexes and could maintain their stability. The transcriptomics analysis results revealed that differentially expressed genes were significantly enriched in the calcium signaling pathway, PI3K-Akt signaling pathway, and MAPK signaling pathway. The results of gene set enrichment analysis for phenotypes revealed that salidroside-added complete nutrition food played a regulatory role in lipid metabolism, glucose metabolism, inflammation, oxidative stress, and fibrosis. It showed a more significant regulation of glucose and lipid metabolism, with the related signaling pathways significantly up-regulated in the test group. In conclusion, salidroside-added complete nutrition food has been found to reduce serum alanine aminotransferase concentration in dogs and help stabilize the concentration of blood indexes in dogs, and regulate hepatic glucolipid metabolism in dogs. These effects may be primarily achieved by regulating the calcium signaling pathway, the PI3K-Akt signaling pathway, and the MAPK signaling pathway.

Key words: salidroside, transcriptomics, glucolipid metabolism, dog

CLC Number:

S858.292

WANG Shengqi, JI Xinyu, HUANG Fuqing, HU Manli, WANG Rouqi, GENG Yuxin, SUN Yingxue, QI Zhili, ZHANG Xin. Effects of Salidroside-added Complete Nutrition Food on Blood Biochemical Indexes and Liver Transcriptomics in Dogs[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(1): 455-465.

Figures/Tables 9

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References 34

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成分 Ingredient	红景天苷全价粮 Salidroside-added complete nutrition food
水分 Moisture	9.23
粗蛋白质 Crude protein	22.33
粗脂肪 Crude fat	10.56
粗纤维 Crude fibre	2.30
粗灰分 Crude ash	4.90
钙 Calcium	0.93
总磷 Total phosphorus	0.55
铜/(mg·kg^-1)Copper	6.37
水溶性氯化物 Water-soluble chloride	0.21
能量/J Gross energy	18 088.67

组别 Group	对照组 Control group	试验组 Test group
体重/kg Body weight	8.76±0.62	8.58±1.36
鼻肛体长/cm Naso-anal length	79.80±4.66	74.20±3.96
腰围/cm Waistline	33.80±2.59	35.80±3.35
臀围/cm Hipline	49.60±5.55	52.80±4.60
背部皮下脂肪厚度/mm Thickness of subcutaneous fat on the back	4.70±0.84	5.00±0.50
BCS评分 BCS score	3.80±0.45	4.20±0.45

指标 Indices	对照组 Control group		试验组 Test group
指标 Indices	第0周	第8周	第0周	第8周
白细胞计数/(10⁹·L^-1) WBC	12.41±1.89	15.94±4.81	10.89±6.34	13.67±2.18
淋巴细胞比例/% LYM	31.28±6.86^a	26.08±6.03	19.33±5.64^b	21.76±7.22
单核细胞比例/% MON	4.33±1.01	3.82±1.22	3.52±2.19	3.88±2.14
中性粒细胞比例/% NEU	59.14±7.41	66.28±6.45	71.35±8.12	70.28±8.44
嗜酸性粒细胞比例/% EOS	3.88±2.34	3.10±2.05	3.10±2.33	2.35±0.50
嗜碱性粒细胞比例/% BAS	0.30±0.19	0.68±0.55	0.84±0.57	0.86±0.38
红细胞计数/(10¹²·L^-1) RBC	9.10±1.54	8.59±0.79	8.55±1.02	9.24±0.37
血小板计数/(10⁹·L^-1) PLT	76.60±55.68^Aa	195.80±69.65^b	242.50±46.68^B	243.00±20.75

指标 Indices	对照组 Control group		试验组 Test group
指标 Indices	第0周	第8周	第0周	第8周
谷丙转氨酶/(U·L^-1)ALT	19.58±7.82	29.10±14.40	34.10±12.36^a	16.96±6.01^b
谷草转氨酶/(U·L^-1)AST	39.34±9.94	32.78±10.62	27.18±6.45	21.30±3.95
碱性磷酸酶/(U·L^-1)ALP	65.44±47.99	39.08±17.14	50.83±13.76	53.52±37.40
血清总蛋白/(g·L^-1)TP	59.62±3.64	55.59±2.44	59.89±5.73	59.25±6.18
白蛋白/(g·L^-1)ALB	37.38±3.85	34.83±2.16	34.60±4.82	35.74±1.61
肌酐/(μmol·L^-1)CREA	64.30±8.43	59.53±0.66	56.07±9.52	67.19±2.07
尿/(mmol·L^-1)UREA	3.61±0.94	4.44±0.77	4.21±1.02	3.78±1.15
总胆固醇/(mmol·L^-1)TC	4.79±0.34	4.19±0.72	3.96±0.72	5.08±1.99
甘油三酯/(mmol·L^-1)TG	0.72±0.02^a	0.52±0.11^b	0.78±0.36	0.75±0.33
肌酸激酶/(U·L^-1)CK	320.60±111.22^a	187.44±55.27^b	155.53±25.08^b	150.20±21.19
α-淀粉酶/(U·L^-1)AMY	852.18±204.53	1124.66±253.84^a	844.62±173.12	768.32±159.89^b

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Effects of Salidroside-added Complete Nutrition Food on Blood Biochemical Indexes and Liver Transcriptomics in Dogs

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