Acta Veterinaria et Zootechnica Sinica ›› 2025, Vol. 56 ›› Issue (5): 2270-2278.doi: 10.11843/j.issn.0366-6964.2025.05.025
• Preventive Veterinary Medicine • Previous Articles Next Articles
WU Feifan1(
), CHANG Weichen1, KONG Liang1, ZHANG Sumei1, LI Lulu1, XU Zenghao1, ZHANG Hongying1, YUAN Shuping2,*(), YANG Mingfan1,*()
Received:2024-06-04
Online:2025-05-23
Published:2025-05-27
Contact:
YUAN Shuping, YANG Mingfan
E-mail:807092852@qq.com;792589939@qq.com;yangmingfan123@163.com
CLC Number:
WU Feifan, CHANG Weichen, KONG Liang, ZHANG Sumei, LI Lulu, XU Zenghao, ZHANG Hongying, YUAN Shuping, YANG Mingfan. Inhibitory Effect of Baicalin on Porcine Reproductive and Respiratory Syndrome Virus in vitro[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(5): 2270-2278.
Fig. 1
Schematic diagram of the different modes of action of baicalin Red columns represent the PRRSV infection period; blue columns represent the baicalin treatment period; and yellow vertical columns represent the end of treatment and cell harvest.-6、-4、-2 hpi represent 6、4、2 h before infected with PRRSV, 2、4、6 hpi represent 2、4、6 h after infected with PRRSV, the same as below"
Fig. 2
Schematic representation of the baicalin treatment at different stages of the replication cycle"
Fig. 3
Effect of different concentrations of baicalin on cell viability of MARC-145 cells"
Fig. 4
Effect of baicalin on PRRSV replication under pre-, co- and post-modes of action A. RT-qPCR for PRRSV ORF7 mRNA; B, C. IFA for PRRSV N protein expression; D. TCID50 for PRRSV virus titers. Bars with different letters mean significant difference among treatments(P < 0.05), while the same letter means no significant difference among treatments(P>0.05). The same as below"
Fig. 5
Effect of baicalin on the invasion, replication and release of PRRSV on MARC-145 cells A, B. PRRSV binding and internalization following baicalin treatment; C, D. PRRSV replication and release following baicalin treatment"
Fig. 6
Effect of baicalin on PRRSV replication after direct interaction A. RT-qPCR test PRRSV ORF7 mRNA; B. TCID50 detect virus titer; C, D. PRRSV N protein expression by IFA, bar=200 μm"
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