参虎败毒颗粒体内抗猪繁殖与呼吸综合征病毒的效果观察

doi:10.11843/j.issn.0366-6964.2021.08.028

Abstract

Abstract: In order to explore the effect of the Shenhu Baidu granules against porcine reproductive and respiratory syndrome virus (PRRSV), 20 piglets free of PRRSV were randomly divided into 4 groups. Group A were given medicine at 3 days before the infection and Group B were given medicine and infection at the same time. Group C and D were the infection and non-infection control, respectively. Medicine were mixed in the basal diet (10 g per pig per day) of A, B groups and supplied from the start time points to the end of the experiment. The rectal temperature of each piglet was recorded everyday. On the 14th day post infection (PI), one piglet in each group was randomly selected and euthanized to observe the lesions caused by viral infection. The viral loads in lungs were detected with RT-qPCR. And on day 1, 3, 5, 7, 14, 21, 28, and 35 PI, anticoagulant and non anticoagulant blood samples were collected for detection viral loads and antibodies, respectively. The rectal temperature of the piglets in Group A and B were raised up to 40℃ at 6th PI and returned to normal at the 11th and 12th days PI. While, high rectal temperature (more than 40℃) of the piglets in Group C lasted to the end of the experiment. The lung lesions in piglets from Group A and B were lighter and with less alveolar structure damage, inflammatory cell invasion and less exudate in the bronchus. However, the lesions in lung from Group C was serious. The alveolar structures were missing and the bronchus in the lung were filled with full of exudates. Serious inflammatory cell invasion were also observed around the bronchus. The viremia of the Group A were highly significantly lower than Group C at 3, 5, 7, and 14 days PI of PRRSV (P<0.01). And Group B was highly significantly lower than Group C at 3, 5 days PI (P<0.01), significantly lower at 7 and 14 days PI (P<0.05). Antibodies against PRRSV in Group A and B reached to the peak at 21 days PI, and the antibodies of the Group C reached to the peak at 28 days PI. In summary, the Shenhu Baidu granules significantly reduced the lesions caused by PRRSV and also the viral loads in bloods and lungs. The drug brought forward of the peak value of PRRSV-specific antibody and reduced the duration of viremia. This study proved that the Shenhu Baidu granules is effective in prevention and treating PRRSV infection.

Key words: Shenhu Baidu granules, porcine reproductive and respiratory syndrome virus, viral load, antibody

CLC Number:

S855.3

LI Chen, QIU Cunyi, FENG Yixue, HAN Likang, DU Lianzhao, WANG Qianqian, MA Xin, LI Ruixiang, WANG Xinglong. Observation of Anti-porcine Reproductive and Respiratory Syndrome Virus Effect of Shenhu Baidu Granules in vivo[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2344-2353.

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Observation of Anti-porcine Reproductive and Respiratory Syndrome Virus Effect of Shenhu Baidu Granules in vivo

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