厚朴酚固体分散体对犊牛生长性能、血清抗氧化能力和肠道微生物的影响

doi:10.11843/j.issn.0366-6964.2025.02.042

Abstract

Abstract:

The purpose of the study was to investigate the effects of magnolol solid dispersion on growth performance, serum antioxidant capacity and gut microorganism of calves. Using the antisolvent co-precipitation method, a solid dispersion of magnolol was prepared using hydroxypropyl acetate methylcellulose succinate as the carrier, with a ratio of 2∶8 between magnolol and the carrier. Fifteen healthy 30 day old Holstein calves with similar physical conditions were randomly divided into 3 groups with 5 replicates in each group. The control group (CON group) was fed pasteurized milk, while the experimental group (MAG group, ASD group) was fed pasteurized milk with 150 mg·d^-1 magnolol and 750 mg·d^-1 solid dispersion of magnolol. The weight of the calves was measured on day 0 and 30 of the experiment, and the average daily weight gain was calculated. On the 30th day of the experiment, rectal feces were collected to detect gut microorganism, jugular vein blood was collected and serum was separated, and antioxidant capacity was measured using ELISA. The characterization results showed that the solid dispersion of magnolol was successfully prepared, and magnolol was dispersed in an amorphous form in the carrier. The pharmacodynamic results showed that magnolol solid dispersion significantly increased final weight and average daily gain of calves (P < 0.05), significantly decreased MDA content (P < 0.05), and significantly increased SOD activity, GSH-Px activity, CAT activity and T-AOC (P < 0.05), significantly increased the relative abundances of Firmicutes and Actinomycetes (P < 0.05), significantly decreased the relative abundances of Proteobacteria (P < 0.05), and significantly increased the Chaol index, Observed_otus (observed feature number), Shannon index and Simpson index (P < 0.05). The effect of magnold ASD was better than magnolol raw material. The results showed that the supplementation of magnolol solid dispersion at 750 mg·d^-1 could improve the growth performance of calves, enhance serum antioxidant capacity, increase the abundance of beneficial bacteria, reduce the abundance of harmful bacteria, increase the diversity of gut microorganism and improve the composition of gut microorganism.

Key words: magnolol solid dispersion, calf, growth performance, serum antioxidant capacity, gut microorganism

CLC Number:

S853.732

ZHANG Yu, WANG Qiru, SHI Xinchao, GUO Ziming, HE Xin, ZHANG Tie, ZHAO Xinghua. Effects of Magnolol Solid Dispersion on Growth Performance, Serum Antioxidant Capacity and Intestinal Microbiome of Calves[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(2): 943-952.

Figures/Tables 9

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References 35

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组别Group	处理Handle
CON	巴氏杀菌乳
MAG	巴氏杀菌乳+厚朴酚150 mg·d ^-1
ASD	巴氏杀菌乳+厚朴酚固体分散体750 mg·d ^-1(含厚朴酚150 mg)

项目 Items	组别Group
项目 Items	CON	MAG	ASD
初重Initial weight	54.14±1.78	56.36±2.69	55.48±2.25
末重Final weight	62.96±1.94^b	65.76±3.01^ab	68.50±3.46^a
平均日增重ADG	0.29±0.04^b	0.31±0.03^b	0.43±0.08^a

项目 Items	组别Group
项目 Items	CON	MAG	ASD
丙二醛/(nmol·mL^-1)MDA	5.54±0.37^a	5.10±0.45^ab	4.67±0.30^b
超氧化物歧化酶/(U·mL^-1)SOD	8.83±0.55^c	10.57±1.46^b	12.74±1.43^a
谷胱甘肽过氧化物酶/(U·mL^-1)GSH-Px	153.46±24.38^c	179.27±30.49^b	202.37±19.22^a
过氧化氢酶/(U·mL^-1)CAT	10.60±0.36^b	10.96±0.33^b	11.62±0.21^a
总抗氧化能力/(U·mL^-1)T-AOC	9.58±0.41^b	10.28±0.40^a	10.69±0.33^a

项目 Items	组别Group
项目 Items	CON	MAG	ASD
厚壁菌门Firmicutes	0.361 6±0.017 9^c	0.576 5±0.023 0^b	0.667 5±0.041 9^a
拟杆菌门Bacteroidota	0.494 4±0.017 5^a	0.359 4±0.015 7^b	0.273 6±0.010 3^c
变形菌门Proteobacteria	0.118 5±0.016 1^a	0.055 1±0.039 0^b	0.016 5±0.003 5^b
放线菌门Actinobacteriota	0.002 8±0.000 2^b	0.017 7±0.000 6^a	0.019 6±0.002 4^a

项目 Items	组别Group
项目 Items	CON	MAG	ASD
Chaol指数Chaol	421.497±6.750^b	436.197±14.608^b	474.080±7.702^a
覆盖度指数Goods-coverage	1.000±0.000	1.000±0.000	1.000±0.000
观测特征数Observed_otus	414.553±9.727^b	427.420±11.556^b	463.860±11.797^a
Shannon指数Shannon	5.777±0.222^b	6.280±0.355^b	6.873±0.167^a
Simpson指数Simpson	0.954±0.006^b	0.960±0.001^b	0.972±0.002^a

Effects of Magnolol Solid Dispersion on Growth Performance, Serum Antioxidant Capacity and Intestinal Microbiome of Calves

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