一株鸽副黏病毒Ⅰ型分离鉴定及致病性分析

doi:10.11843/j.issn.0366-6964.2024.09.029

Abstract

Abstract:

A strain of pigeon paramyxovirus-1(PPMV-1) was isolated from the dead pigeon tissue in Tianjin region. To provide scientific basis for the development of PPMV-1 vaccines, the genetic evolution, biological characteristics, and pathogenicity of the virus were analyzed. The pathogens were identified by RT-PCR, virus isolation, purification by chicken embryos, genetic evolution analysis, pathogenicity analysis, and animal regression tests. A strain of PPMV-1 was isolated from the dead pigeons and named Pigeon/TJ/CH/020/2020 (TJ20). Sequence analysis and homology analysis of the virus F gene showed that the strain belonged to sub-genotype Ⅵ.2.1.1.2.2(Ⅵk), and the amino acid residues of the cleavage site were ¹¹²R-R-Q-K-R-F¹¹⁷, which were consistent with the molecular characteristics of NDV virulent strains. Hemagglutination inhibition revealed a noticeable difference between TJ20 and LaSota. The values of TCID₅₀, EID₅₀, MDT and ICPI were 10^-8.64·0.1 mL^-1, 10^-8.38·0.1 mL^-1, 62 h, 1.19. The animal infection experiment found that pigeons showed clinical symptoms on 4 dpc (day 4 post-challenge). The incidence rate and mortality were 100%, and the cloacal detoxification rate could reach 100% on 5 dpc. TJ20 was highly pathogenic to pigeons. This study successfully isolated a highly pathogenic sub-genotype Ⅵ.2.1.1.2.2(Ⅵk) TJ20 strain from samples of dead pigeons, providing important biological materials for the research and development of vaccine of PPMV-1 in the future.

Key words: pigeon paramyxovirus-1, isolation and identification, sub-genotype Ⅵ.2.1.1.2.2(Ⅵk), pathogenicity

CLC Number:

S852.659.5

Shan ZHANG, Dahu LIU, Baojing LIU, Lin LIANG, Ruiying LIANG, Xinming TANG, Xusheng QIU, Chan DING, Jiabo DING, Shaohua HOU. Isolation, Identification and Pathogenicity Analysis of a Pigeon Paramyxovirus-1 Strain[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 4051-4060.

Figures/Tables 8

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引物名称Primer name	引物序列(5′→3′) Nucleotide sequence	产物大小/bp Product size
NDV-F	CGAAGAGCCCGTTAGTCAAATC	256
NDV-R	GTATCTTGGCAACCTGGGGAG
ⅥF	GCTTATAGTCAGTTTACCTGCC	1 931
ⅥR	GTGGTAGAACAGATGTTGTGAAG

组别Group	毒株Strain	数量/只Number	口咽Oropharyngeal				泄殖腔Cloacal
组别Group	毒株Strain	数量/只Number	第3天3 dpc	第5天5 dpc	第7天7 dpc	第10天10 dpc	第3天3 dpc	第5天5 dpc	第7天7 dpc	第10天10 dpc
感染组Infection group	TJ20	10	0/10	3/10	3/9	2/3	1/10	10/10	9/9	3/3
对照组Control group	/	10	0/10	0/10	0/10	0/10	0/10	0/10	0/10	0/10

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Isolation, Identification and Pathogenicity Analysis of a Pigeon Paramyxovirus-1 Strain

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