猪链球菌SC124菌株中ICE_Prophage携带耐药基因的水平转移

doi:10.11843/j.issn.0366-6964.2024.08.041

Abstract

Abstract:

This study aimed to investigate the transfer of antibiotic resistance genes (ARGs) carried by ICE_Prophage in Streptococcus suis. The whole genome sequencing of S. suis SC124 was performed using the Illumina MiSeq and Pacbio RSII platforms. The whole genome sequence of SC124 isolates was analyzed by online websites RAST, ICEberg 3.0, PHASTER and NCBI database. MICs were determined by broth microdilution. The transferability of ICE_Prophage carrying ARGs in S. suis was investigated by conjugation. The present of circular intermediates was examined by inverse PCR. Competition was used to evaluate whether the transconjugant had fitness cost. The results showed that there was an ICE_Prophage (ICESsuSC124_ΦSsuSC124) carrying tetracycline, macrolide and aminoglycoside resistance genes in S. suis SC124. ICESsuSC124 carrying tetracycline resistance genes was transferred at a frequency of 5.52×10^-9, and no prophage transfer was found. Inverse PCR revealed that ICESsuSC124 formed a circular intermediate with a size of about 3.8 kb. Compared with S. suis BAA, the transconjugant containing ICESsuSC124 did not have a competitive advantage under the action of drug-free pressure selection, and the transconjugant can produce a certain degree of fitness cost. In conclusion, the ICE_Prophage carrying tetracycline, macrolide and aminoglycoside resistance genes in S. suis SC124, in which ICESsuSC124 could be horizontally transferred, and the transconjugant could produce a certain degree of fitness cost.

Key words: Streptococcus suis, ICE_Prophage, conjugation, fitness cost

CLC Number:

S852.611

Huihui DUAN, Shihang REN, Hongyin ZHANG, Rui YU, Zhonghu LIU, Xiangdang DU, Yanhong SHANG. Horizontal Transfer of Drug Resistance Genes Carried by ICE_Prophage in Streptococcus suis SC124[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3699-3705.

Figures/Tables 5

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References 19

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菌株Strains	att1	att2	att3
TZ080501	catCAC$\underline{\rm{G}}$T$\underline{\rm{G}}$GA$ \underline {{\rm{gtg}}} $tgt	ccaCAC$\underline{\rm{G}}$T$\underline{\rm{G}}$GA$ \underline {{\rm{gtg}}} $cgt	acaCAC$\underline{\rm{A}}$T$\underline{\rm{A}}$GAagttgt
SC070731	catCAC$\underline{\rm{G}}$T$\underline{\rm{G}}$GA$ \underline {{\rm{gtg}}} $cgt	acaCACGT$\underline{\rm{G}}$GA$ \underline {{\rm{gtg}}} $tgt	ccaCAC$\underline{\rm{A}}$T$\underline{\rm{A}}$GAagttgt
SC124	catCAC$\underline{\rm{G}}$T$\underline{\rm{G}}$GA$ \underline {{\rm{gtg}}} $cgt	acaCAC$\underline{\rm{G}}$T$\underline{\rm{G}}$GA$ \underline {{\rm{gtg}}} $tgt	ccaCAC$\underline{\rm{A}}$T$\underline{\rm{A}}$GAagttgt

菌株 Strains	MICs
菌株 Strains	CLI	LIN	ERY	RFP	FUS	TET	CHL	FFC	AMP
SC124	128	256	256	＜1	4	128	2	＜1	＜1
BAA	＜1	＜1	＜1	128	64	＜1	＜1	＜1	＜1
BAA+ICESsuSC124	＜1	＜1	＜1	128	64	128	＜1	＜1	＜1

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Horizontal Transfer of Drug Resistance Genes Carried by ICE_Prophage in Streptococcus suis SC124

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