禽白血病病毒J亚群感染鸡组织中细胞受体chNHE1的表达分析

doi:10.11843/j.issn.0366-6964.2024.08.034

Abstract

Abstract:

This study aimed to investigate the expression of chicken sodium hydrogen exchanger isoform 1 (chNHE1) in different types of chicken tissues, the tissue tropism of subgroup J avian leukosis virus (ALV-J), and the changes in mRNA of chNHE1 after ALV-J infection. We used qPCR to detect the transcription of chNHE1 in the organs of commercial broilers, commercial layers, and SPF chickens, as well as the viral load in various tissues and the changes in chNHE1 transcription in tissues after ALV-J infection. The results showed that chNHE1 was transcripted in the heart, liver, spleen, lung, kidney, cecal tonsil, thymus, and bursa in all three types of chickens, but the transcription levels varied greatly, with relatively higher transcription levels in the immune organs. After SPF chickens were infected with ALV-J, the viral load was relatively higher in the heart, spleen, kidney, and cecal tonsils, while it was relatively lower in the liver, lung, thymus, and bursa. The transcription of chNHE1 in the spleen, kidney, and cecal tonsils was significantly upregulated after ALV-J infection, but there was no significant change in the transcription in the heart compared to the control group. This study analyzed the tissue transcription characteristics of chNHE1 in three types of chicken and the effect of ALV-J infection on the transcription chNHE1 expression in tissues, providing an important theoretical basis for exploring the relationship between the expressiontranscription of the receptor chNHE1 and the tissue tropism of ALV-J.

Key words: subgroup J avian leukosis virus, chicken sodium hydrogen exchanger isoform 1, tissue expression, transcriptional changes

CLC Number:

S852.659.3

Li ZHANG, Mengmeng YU, Ying WANG, Suyan WANG, Zhuangzhuang XU, Peng LIU, Yuntong CHEN, Xiaole QI, Liuan LI, Yulong GAO. Analysis of Cellular Receptor chNHE1 Expression in Chicken Tissues Infected with Avian Leukosis Virus Subgroup J[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3631-3639.

Figures/Tables 5

Table 1

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References 31

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引物名称 Primer	引物序列(5′→3′) Primer sequence	片段长度/bp Fragment length
chNHE1-F	CTGAACAAACAGCACCACAAC	291
chNHE1-R	GAGGAGGAAGAGGAAGAAGATG
GAPDH-F	CCCCAATGTCTCTGTTGTTGAC	295
GAPDH-R	CAGCCTTCACTACCCTCTTGAT
ALV-J-ENV-F	TTGCAGGCATTTCTGACTGG	214
ALV-J-ENV-R	ACACGTTTCCTGGTTGTTGC
OVO-F	CACTGCCACTGGGCTCTGT	71
OVO-R	GCAATGGCAATAAACCTCCAA

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Analysis of Cellular Receptor chNHE1 Expression in Chicken Tissues Infected with Avian Leukosis Virus Subgroup J

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