牛支原体能量耦合因子转运蛋白S组件MbBioY摄取外源生物素的功能分析

doi:10.11843/j.issn.0366-6964.2024.01.026

Abstract

Abstract: M. bovis infection and survival depend on various nutritional and metabolic factors provided by the external microenvironment. The study of the mechanism of exogenous biotin uptake by M. bovis will be of substantial significance to M. bovis prevention and control. An examination of the M. bovis PG45 MbBioY (MBOVPG45_0349) gene was used for phylogenetic analysis and molecular docking analysis; Screening of M. bovis PG45 ΔMbBioY mutant; to compare the biotin sensitivity of PG45 and PG45 ΔMbBioY the function of M.bovis was further verified by heterologous construction of MbBioY functional clone. The results showed that a novel biotin membrane transporter MbBioY was found on the chromosome of PG45. It was 996 bp in length, 30.95% GC content, and 332 amino acids in length. The protein consists of seven transmembrane domains. Further verification revealed that MbBioYloss affected M. bovis growth and showed phenotypic biotin metabolism deficiencies. By heterologous knock-in of biotin-metabolizing nutrient-deficient E. coli MG1655, it was shown that MbBioY alone could restore the physiological function of the nutrient-deficient E. coli strain with biotin metabolism. It has been preliminarily demonstrated that the S component of the energy coupling factor (ECF) transporter encoded by MbBioY can absorb exogenous biotin. This research result will contribute to the diversity and complexity of bacterial biotin metabolism regulation. M. bovis prevention and control will be provided from an innovative perspective.

Key words: Mycoplasma bovis, energy-coupled factor transporter, biotin, transport

CLC Number:

S852.62

CHEN Qiwei, QUAN Heng, CHEN Shengli, LIU Donghui, YU Yongfeng, LI Caiyu, GONG Xiaowei, CHU Yuefeng. Functional Analysis of Biotin Uptake by the Energy-coupled Factor Transporter S Component MbBioY of Mycoplasma bovis[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 282-289.

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Functional Analysis of Biotin Uptake by the Energy-coupled Factor Transporter S Component MbBioY of Mycoplasma bovis

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