豪猪血蜱卵蛋白成分初步鉴定及其功能分类

doi:10.11843/j.issn.0366-6964.2021.010.020

Abstract

Abstract: The identification of protein components of Haemaphysalis hystricis egg protoplasm illustrated the tick embryo protein profile and laid a foundation for the discovery of new anti-tick strategies. The egg protein extracts of Haemaphysalis hystricis was digested with trypsin according the FASP procedure and the LC/MS/MS method was employed to detect and identify peptides. The MS data were searched against the ovarian, salivary gland and midgut transcriptomic libraries of Haemaphysalis flava, and the 359, 312 and 357 specific peptides were detected, then the 108, 170 and 161 polypeptides were identified. Eventrually, the 103 proteins of them were considered as high confidence proteins (peptides ≥ 2). These high confidence proteins were involved in eight groups including enzymes, protease inhibitors, transporters, cytoskeleton proteins, protein synthesis modifications, secreted proteins and uncharacterized proteins.

Key words: egg, LC-MS/MS, protease, transporter, protease inhibitor

CLC Number:

S852.746

LIU Jinbao, CHENG Tianyin. Preliminary Identification and Functional Classification of Haemaphysalis hystricis Egg Protein Components[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(10): 2895-2904.

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Preliminary Identification and Functional Classification of Haemaphysalis hystricis Egg Protein Components

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