牛胚胎基因组选择研究进展

doi:10.11843/j.issn.0366-6964.2023.11.002

Abstract

Abstract: Bovine embryonic genome selection (EGS) refers to screening out high-quality early embryos by biopsying the partial trophoblast cells in the early embryo and evaluating the genetic breeding value through the whole genome amplification of micro embryonic cells. The primary steps of this technique include:early bovine blastocysts around day 6 were biopsied and micro trophoblast cells were extracted using a micromanipulation system, followed by whole genomic amplification. Single-nucleotide polymorphism analysis predicts the early embryo production performance, and thus selects the embryos with good genotypes for transplantation. This paper summarizes the biopsy methods and amplification methods commonly used in EGS technology, and the application status of this technology in cattle and the problems of this technology were outlined, so as to provide reference for the development of EGS technology in the future.

Key words: embryonic genome selection, biopsy method, amplification method, breeding

CLC Number:

S823.2

NIU Yifan, YANG Baigao, ZHANG Peipei, ZHANG Hang, FENG Xiaoyi, CAO Jianhua, YU Zhou, HAO Haisheng, DU Weihua, ZOU Huiying, ZHU Huabin, MA Youji, ZHAO Xueming. Advances in Bovine Embryo Genome Selection[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4449-4457.

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Advances in Bovine Embryo Genome Selection

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