泌乳阶段和处理温度对驴乳中溶菌酶活性的影响

doi:10.11843/j.issn.0366-6964.2022.04.009

Abstract

Abstract: The study aimed to explore the relationship between lysozyme activity in donkey milk and lactation stages and treatment temperature. The lysozyme activities in fresh milk of cow, sheep and donkey were compared. To investigate the effects of lactation stages and lactation level on lysozyme activity in milk，female donkeys aged 4-9 years were divided into 3 groups according to the average daily milk yield: low (below 400 g), medium (400-1 000 g) and high (above 1 000 g) groups，3 animals in each group, and 9 animals in total were used to observe the lysozyme activity in the milk of female donkeys from the 3rd to 10th lactation month regular continuous sampling every month for 7 days. Finally, mixed donkey milk obtained on 5 sampling days was divided into 6 aliquots, one of which was not treated, and the others were heated at 62 ℃ for 30 min, 72, 77, 82 and 87 ℃ for 15 s, respectively. Lysozyme activity was detected after cooling to study the effect of heat treatment condition on lysozyme activity in milk. The results showed that the lysozyme activity in donkey milk (12 367 U·mL^-1) was significantly higher than that in cow milk (334 U·mL^-1) and sheep milk (233 U·mL^-1) (P<0.05). Lysozyme activity in milk secreted by female donkeys at the 8th lactation month after parturition was the highest (14 383 U·mL^-1) (P<0.05). During the processing of raw milk, sterilization at 62 ℃ for 30 min had no significant effect on lysozyme activity (P>0.05), while sterilization at 72-87 ℃ for 15 s significantly decreased lysozyme activity (P<0.05). Compared with cow and sheep milk, donkey milk has higher lysozyme activity, and long-term sterilization at low temperature does not affect the lysozyme activity.

Key words: donkey milk, lysozyme, antibacterial activity, pasteurization

CLC Number:

S822.1

WANG Han, HU Yuling, ZHAO Chenkun, YANG Zhipeng, ZENG Shenming. Effects of Lactation Stages and Heat Treatment Temperature on Lysozyme Activity in Donkey Milk[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1089-1095.

References 31

[1]	ALHAZMI A,STEVENSON J,AMARTEY S,et al.Discovery,modification and production of T4 lysozyme for industrial and medical uses[J].Int J Biol, 2014,6(4):45-63.
[2]	RAGLAND S A,CRISS A K.From bacterial killing to immune modulation:recent insights into the functions of lysozyme[J].PLoS Pathog, 2017,13(9):e1006512.
[3]	TIDONA F,CRISCIONE A,DEVOLD T G,et al.Protein composition and micelle size of donkey milk with different protein patterns:effects on digestibility[J].Int Dairy J, 2014,35(1):57-62.
[4]	LEE W,KU S K,NA D H,et al.Anti-inflammatory effects of lysozyme against HMGB1 in human endothelial cells and in mice[J].Inflammation, 2015,38(5):1911-1924.
[5]	MAO X Y,GU J N,SUN Y,et al.Anti-proliferative and anti-Tumour effect of active components in donkey milk on A549 human lung cancer cells[J].Int Dairy J, 2009,19(11):703-708.
[6]	GOTTLIEB M G V,CLOSS V E,JUNGES V M,et al.Impact of human aging and modern lifestyle on gut microbiota[J].Crit Rev Food Sci Nutr, 2018,58(9):1557-1564.
[7]	JIANG L,LV J Y,LIU J W,et al.Donkey milk lysozyme ameliorates dextran sulfate sodium-induced colitis by improving intestinal barrier function and gut microbiota composition[J].J Funct Foods, 2018,48:144-152.
[8]	OZTURKOGLU-BUDAK S,AKAL H C,BERELI N,et al.Use of antimicrobial proteins of donkey milk as preservative agents in Kashar cheese production[J].Int Dairy J, 2021,120:105090.
[9]	POLIDORI P,BEGHELLI D,MARIANI P,et al.Donkey milk production:state of the art[J].Ital J Anim Sci, 2009,8(S2):677-683.
[10]	SPADA V,FERRANTI P,CHIANESE L,et al.Antibacterial potential of donkey’s milk disclosed by untargeted proteomics[J].J Proteomics, 2021,231:104007.
[11]	CUNSOLO V,SALETTI R,MUCCILLI V,et al.Proteins and bioactive peptides from donkey milk:the molecular basis for its reduced allergenic properties[J].Food Res Int, 2017,99:41-57.
[12]	SOUROULLAS K,ASPRI M,PAPADEMAS P.Donkey milk as a supplement in infant formula:Benefits and technological challenges[J].Food Res Int, 2018,109:416-425.
[13]	MARTINI M,SALARI F,LICITRA R,et al.Lysozyme activity in donkey milk[J].Int Dairy J, 2019,96:98-101.
[14]	PILLA R,DAPRÀ V,ZECCONI A,et al.Hygienic and health characteristics of donkey milk during a follow-up study[J].J Dairy Res, 2010,77(4):392-397.
[15]	GAO A,MUTHARIA L,CHEN S,et al.Effect of pasteurization on survival of Mycobacterium paratuberculosis in milk[J].J Dairy Sci, 2002,85(12):3198-3205.
[16]	VINCENZETTI S,SAVINI M,CECCHINI C,et al.Effects of lyophilization and use of probiotics on donkey’s milk nutritional characteristics[J].Int J Food Eng, 2011,7(5):8.
[17]	POLIDORI P,VINCENZETTI S.Differences of protein fractions among fresh,frozen and powdered donkey milk[J].Recent Pat Food Nutr Agric, 2010,2(1):56-60.
[18]	ADDO C,FERRAGUT V.Evaluating the Ultra-High Pressure Homogenization (UHPH) and Pasteurization effects on the quality and shelf life of donkey milk[J].Int J Food Stud, 2015,4(1):104-115.
[19]	COPPOLA R,SALIMEI E,SUCCI M,et al.Behaviour of Lactobacillus rhamnosus strains in ass’s milk[J].Ann Microbiol, 2002, 52(1):55-60.
[20]	KRÓL J,BRODZIAK A,LITWI AN＇U CZUK A.Basic chemical composition and content of selected whey proteins in milk from different cow breeds and in rennet whey[J].Food Sci Technol Quality, 2011,4(77):74-83.
[21]	BRODZIAK A,KRÓL J,BARŁOWSKA J,et al.Effect of production season on protein fraction content in milk of various breeds of goats in Poland[J].Int J Dairy Technol, 2014,67(3):410-419.
[22]	MARSZAŁKOWSKA J,GUTOWSKA J,SAMOLI AŃU SKI B.Frequency of positive skin prick tests to food allergens in an allergy outpatient clinic[J].Alergia Astma Immunol, 2007,12(3):160-164.
[23]	POLIDORI P,VINCENZETTI S.Use of Donkey milk in children with cow’s milk protein allergy[J].Foods, 2013,2(2):151-159.
[24]	CLAEYS W L,VERRAES C,CARDOEN S,et al.Consumption of raw or heated milk from different species:an evaluation of the nutritional and potential health benefits[J].Food Control, 2014,42:188-201.
[25]	CONTE F,FOTI M,MALVISI M,et al.Valutazione dell’azione antibattericadel lisozima del latte d’asina.Considerazioni igienico-sanitarie[J].Large Anim Rev, 2012:18:13-16.
[26]	YVON S,SCHWEBEL L,BELAHCEN L,et al.Effects of thermized donkey milk with lysozyme activity on altered gut barrier in mice exposed to water-avoidance stress[J].J Dairy Sci, 2019,102(9):7697-7706.
[27]	LÖNNERDAL B,ERDMANN P,THAKKAR S K,et al.Longitudinal evolution of true protein,amino acids and bioactive proteins in breast milk:a developmental perspective[J].J Nutr Biochem, 2017,41:1-11.
[28]	MONTAGNE P,CUILLIōRE M L,MOLÉ C,et al.Changes in lactoferrin and lysozyme levels in human milk during the first twelve weeks of lactation[J].Adv Exp Med Biol, 2001,501:241-247.
[29]	KOENIG Á,DE ALBUQUERQUE DINIZ E M,BARBOSA S F C,et al.Immunologic factors in human milk:the effects of gestational age and pasteurization[J].J Hum Lact, 2005,21(4):439-443.
[30]	CHIAVARI C,COLORETTI F,NANNI M,et al.Use of donkey’s milk for a fermented beverage with lactobacilli[J]. Lait, 2005, 85:481-490.
[31]	SOUSA S G,DELGADILLO I,SARAIVA J A.Effect of thermal pasteurisation and high-pressure processing on immunoglobulin content and lysozyme and lactoperoxidase activity in human colostrum[J].Food Chem, 2014,151:79-85.

Effects of Lactation Stages and Heat Treatment Temperature on Lysozyme Activity in Donkey Milk

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 31

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WU Zhouhui, WANG Yu, DU Heng, WANG Zhiwen, XIAO Shuang, WU Jinliang, WANG Zhen. Analysis of the Antibacterial Sensitization Activity of Tirapazamine against Multi-Drug Resistant Salmonella [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4362-4371.
[2]	TIAN Wei, LI Xiumei, YANG Juan, WANG Xiaoying, ZHOU Weiwei, LI Zhongyuan, DAI Xiaofeng. Network Pharmacology Study on the Antibacterial Activity Components and Mechanism of Isatis indigotica [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2782-2793.
[3]	BAI Yu, WANG Wu, WANG Bi, LI Ying, FENG Zili. The Cytotoxicity of Amyloid Fibrils Formed by Hen Egg-White Lysozyme on SH-SY5Y Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2721-2728.
[4]	LI Ying, FENG Zili, BAI Yu, ZHANG Yuhang, REN Xueyi. The Modification of Hen Egg-White Lysozyme and Its Antibacterial Activity [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 1094-1102.
[5]	GAO Yue, WANG Wanlin, PENG Junchao, CAI Yaling, LIAO Jiamei, YI Qiong, WANG Lu. Screening of Antibacterial Activity of Artemisia Oil from Different Sources and Preliminary Study on Its Antibacterial Activity against Staphylococcus aureus [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(2): 525-534.
[6]	LI Nan, ZHANG Jianlou, ZHANG Yonghong, ZHENG Zhiqiang, HUO Shanshan, ZHONG Fei, ZHAI Xianghe. Preparation of Bovine Surfactant Protein A and Its Antibacterial Activities [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(2): 498-505.
[7]	WU Yiran, GU Xiaobin, XIE Yue, YANG Guangyou. Prokaryotic Expression and Primary Evaluation of Antibacterial Activity of Antibacterial Factor in Baylisascaris schroederi [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 151-158.
[8]	ZHOU Xiao-ling，FANG Lei，WANG Lian-qun，CHEN Gen-yuan，DOU Quan-lin. Effects of Cecal Infusion of Different SCFA on Donkey Milk Performance and Milk Fatty Acid Profile [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1422-1427.
[9]	WEI Xiao-xiao，HANG Bo-lin，MA Cui，XIA Yi-he，ZHANG Bing-qing，WANG Qing，XU Yan-zhao，HU Jian-he. Analysis of Antimicrobial Activity and Stability of Antibacterial Peptide JH-3 [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 361-366.
[10]	FENG Pei-xiang，ZHU Mei-sheng，YANG Li，TAN Xue-jin，PAN Fu-xing，QI Juan，GUO Yan-yan，ZHANG Yi，YIN Yan-bo. cDNA Cloning，Expression and Antibacterial Activity Analysis of Chicken β-defensin Gal-9 [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(6): 997-1003.
[11]	LIU Xiao-ye, ZHANG Tao, DONG Hong, DUAN Hui-qin, HU Ge, MU Xiang. Effects of Pulsatilla Decoction on the Lysozyme of Transendothelial Migration of Polymorph Nuclear Neutrophils in Rat [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(1): 142-146.
[12]	CHEN Ji-Ping, ZHAO Bao-Yu, LU Hao, DING Lai-Di, ZHANG Guang-Fang, LI Xiao-Meng, GAO Yuan, ZHOU Qi-Wu, YANG Xiao-Wen. Study on Isolation, Identification and the Antibacterial Activity of Endophytic Fungi from the Major Locoweed in China [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(9): 1471-1478.
[13]	ZANG Chang-jiang, WANG Jia-qi, YANG Yong-xin, BU Deng-pan, YANG Jin-hui, YUAN Ting-jie, ZHOU Ling-yun, SUN Peng, LI Fa-di. Comparative Proteomics Analysis of Changes of the Milk Protein during Thermal Treatment [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(11): 1754-1759.
[14]	LIN Li-juan;HAN Zong-xi;SHAO Yu-hao;ZHANG Ke-xin; LIU Sheng-wang;LI Zi-rui;MA De-ying . cDNA Cloning, Expression and Initial Characterization of Antibacterial Activity of DuckAvian Beta-defensin 4 [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2011, 42(9): 1309-1315.
[15]	GAO Xiao-yan;WANG Chang-fa;LIU Shun-de;LI Qiu-ling;YANG Hong-jun;YANG Shao-hua;ZHONG Ji-feng;HE Hong-bin . Cloning， Expression of Bovine Tracheal Defensin in E.coli and Its Antibacterial Activity and Tissue Distribution [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(9): 1166-1171.