基于网络药理学分析桑叶增强鸡抗氧化功能的作用机制

doi:10.11843/j.issn.0366-6964.2022.06.029

Abstract

Abstract: This study aims to analyze the mechanism of Mori Folium enhancing antioxidative function of chicken. The chemical components and targets of Mori Folium were collected by the traditional Chinese medicine database and analysis platform (TCMSP) and herb database. The compound-target network and the protein-protein interaction (PPI) network of Mori Folium were constructed and analyzed by Cytoscape3.7.2 software, NCBI database and STRING database. In addition, GO enrichment and KEGG pathway of these targets in PPI network were analyzed. The results showed that a total of 30 active compounds were screened, including quercetin, rutin, kaempferol, β-sitosterol, β-carotene and arachidonic acid, which acted on 221 targets. Among them, IL-6, VEGFA, EGF, INS, CAT, CASP3, CCND1, PTGS2, IL-1B and MMP9 were the core targets. GO enrichment and KEGG pathway analysis showed that the role of the targets involved in molecular function, biological process and cell composition, and involved in a number of metabolic pathways such as NOD-like receptor signaling pathway, PPAR signaling pathway, Toll-like receptor signaling pathway and apoptosis. This study theoretically shows that Mori Folium can regulate the antioxidation of chicken based on targets including IL-6, VEGFA, EGF, INS, CAT, CASP3, CCND1, PTGS2, IL-1B and MMP9, which provides enlightenment for further experimental verification and the development of Mori Folium as antioxidation feed additives.

Key words: Mori Folium, chicken, antioxidation, oxidative stress, network pharmacology, target

CLC Number:

R285
S831.5

LIU Weiwei, ZHANG Yuxin, LI Xiumei, WANG Xiumin, ZHOU Weiwei, DAI Xiaofeng. Mechanism of Mori Folium in Improving Antioxidative Function of Chicken Based on Network Pharmacology[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1958-1970.

References 48

[1]	REUTER S, GUPTA S C, CHATURVEDI M M, et al. Oxidative stress, inflammation, and cancer: how are they linked?[J]. Free Radic Biol Med, 2010, 49(11): 1603-1616.
[2]	SURAI P F, KOCHISH I I, FISININ V I, et al. Antioxidant defence systems and oxidative stress in poultry biology: an update[J]. Antioxidants (Basel), 2019, 8(7): 235.
[3]	LEE M T, LIN W C, YU B, et al. Antioxidant capacity of phytochemicals and their potential effects on oxidative status in animals-A review[J]. Asian-Australas J Anim Sci, 2017, 30(3): 299-308.
[4]	SINGH R, BAGACHI A, SEMWAL A, et al. Traditional uses, phytochemistry and pharmacology of Morus alba Linn. : A review[J]. J Med Plants Res, 2013, 7(9): 461-469.
[5]	GRYN-RYNKO A, BAZYLAK G, OLSZEWSKA-SLONINA D. New potential phytotherapeutics obtained from white mulberry (Morus alba L. ) leaves[J]. Biomed Pharmacother, 2016, 84: 628-636.
[6]	LIU Y Y, LI Y H, XIAO Y, et al. Mulberry leaf powder regulates antioxidative capacity and lipid metabolism in finishing pigs[J]. Anim Nutr, 2021, 7(2): 421-429.
[7]	WANG B, YANG C T, DIAO Q Y, et al. The influence of mulberry leaf flavonoids and Candida tropicalis on antioxidant function and gastrointestinal development of preweaning calves challenged with Escherichia coli O141: K99[J]. J Dairy Sci, 2018, 101(7): 6098-6108.
[8]	XU Z Y, LEI C L, LI J, et al. Effects of mulberry leaf meal on serum antioxidant indexes and immune indexes of laying hens[J]. Animal Husbandry & Veterinary Medicine, 2017, 49(12): 34-38. (in Chinese)许祯莹, 雷春龙, 李娟, 等. 日粮中添加桑叶粉对蛋鸡血液抗氧化和免疫指标的影响[J]. 畜牧与兽医, 2017, 49(12): 34-38.
[9]	LIN W C, LEE M T, CHANG S C, et al. Effects of mulberry leaves on production performance and the potential modulation of antioxidative status in laying hens[J]. Poult Sci, 2017, 96(5): 1191-1203.
[10]	TILAHUN M, URGE M, YIRGA M. Effect of substituting commercial feed with mulberry leaf meal on performance of broiler chickens[J]. J Biol Agric Healthc, 2018, 8(14): 58-63.
[11]	HOPKINS A L. Network pharmacology[J]. Nat Biotechnol, 2007, 25(10): 1110-1111.
[12]	LI S, ZHANG B. Traditional Chinese medicine network pharmacology: theory, methodology and application[J]. Chin J Nat Med, 2013, 11(2): 110-120.
[13]	WANG C H, SUN X F. The target and pathway of Gegen Qinlian decoction in treating swine diarrhea based on network pharmacology[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(11): 2875-2885. (in Chinese)王春花, 孙雪芳. 基于网络药理学探讨葛根芩连汤治疗猪腹泻的靶点与通路[J]. 畜牧兽医学报, 2020, 51(11): 2875-2885.
[14]	LIU X X, LIU M J, YIN P, et al. A review on the traditional Chinese veterinary medicine network pharmacology[J]. Acta Veterinaria et Zootechnica Sinica, 2014, 45(6): 859-862. (in Chinese)刘晓曦, 刘明江, 尹朋, 等. 中兽药网络药理学研究进展[J]. 畜牧兽医学报, 2014, 45(6): 859-862.
[15]	DONG Z, BAI D D, LIU L L, et al. The mechanism of Gui Qi Yimu decoction powder in treating cow qi and blood two deficiency syndrome based on network pharmacology[J]. Acta Veterinaria et Zootechnica Sinica, 2018, 49(12): 2733-2744. (in Chinese)董朕, 白东东, 刘利利, 等. 基于网络药理学分析归芪益母汤治疗牛气血两虚证的作用机制[J]. 畜牧兽医学报, 2018, 49(12): 2733-2744.
[16]	MEMON A A, MEMON N, LUTHRIA D L, et al. Phenolic acids profiling and antioxidant potential of mulberry (Morus laevigata W., Morus nigra L., Morus alba L. ) leaves and fruits grown in Pakistan[J]. Pol J Food Nutr Sci, 2010, 60(1): 25-32.
[17]	YAO L, HU D N, CHEN M, et al. Subtoxic levels hydrogen peroxide-induced expression of interleukin-6 by epidermal melanocytes[J]. Arch Dermatol Res, 2012, 304(10): 831-838.
[18]	KWEIDER N, FRAGOULIS A, ROSEN C, et al. Interplay between vascular endothelial growth factor (VEGF) and nuclear factor erythroid 2-related factor-2 (Nrf2): implications for preeclampsia[J]. J Biol Chem, 2011, 286(50): 42863-42872.
[19]	TANG X P, LIU B, WANG X R, et al. Epidermal growth factor, through alleviating oxidative stress, protect IPEC-J2 cells from lipopolysaccharides-induced apoptosis[J]. Int J Mol Sci, 2018, 19(3): 848.
[20]	TANG X P. The study of influences of epidermal growth factor on intestinal barrier function and phosphorus absorption of weaned piglets[D]. Changsha: Hunan Agricultural University, 2018. (in Chinese)汤小朋. 表皮生长因子对脂多糖诱导的仔猪肠道屏障功能及磷吸收影响研究[D]. 长沙: 湖南农业大学, 2018.
[21]	WANG X, WU H, CHEN H L, et al. Does insulin bolster antioxidant defenses via the extracellular signal-regulated kinases-protein kinase B-nuclear factor erythroid 2 p45-related factor 2 pathway?[J]. Antioxid Redox Signal, 2012, 16(10): 1061-1070.
[22]	TANG M H. Effects of catalase on growth performance, intestinal morphology and antioxidant capacity in yellow broilers[J]. Chinese Journal of Animal Nutrition, 2021, 33(7): 4153-4161. (in Chinese)唐明红. 过氧化氢酶对黄羽肉鸡生长性能、肠道形态以及抗氧化能力的影响[J]. 动物营养学报, 2021, 33(7): 4153-4161.
[23]	WANG F, FU C X, CHEN J S, et al. Biological function of catalase and its application in animals[J]. Feed Research, 2021, 44(5): 126-129. (in Chinese)王芳, 符晨星, 陈家顺, 等. 过氧化氢酶的生物学功能及在动物中的应用[J]. 饲料研究, 2021, 44(5): 126-129.
[24]	TIAN D S, LIU J J, CHEN L, et al. The protective effects of PI3K/Akt pathway on human nucleus pulposus mesenchymal stem cells against hypoxia and nutrition deficiency[J]. J Orthop Surg Res, 2020, 15(1): 29.
[25]	BURCH P M, HEINTZ N H. Redox regulation of cell-cycle re-entry: cyclin D1 as a primary target for the mitogenic effects of reactive oxygen and nitrogen species[J]. Antioxid Redox Signal, 2005, 7(5-6): 741-751.
[26]	WONG W T, TIAN X Y, CHEN Y C, et al. Bone morphogenic protein-4 impairs endothelial function through oxidative stress-dependent cyclooxygenase-2 upregulation: implications on hypertension[J]. Circ Res, 2010, 107(8): 984-991.
[27]	KIRITOSHI S, NISHIKAWA T, SONODA K, et al. Reactive oxygen species from mitochondria induce cyclooxygenase-2 gene expression in human mesangial cells: potential role in diabetic nephropathy[J]. Diabetes, 2003, 52(10): 2570-2577.
[28]	CHOWDHURY T, ALLEN M F, THORN T L, et al. Interleukin-1β protects neurons against oxidant-induced injury via the promotion of astrocyte glutathione production[J]. Antioxidants (Basel), 2018, 7(8): 100.
[29]	HE Y, JACKMAN N A, THORN T L, et al. Interleukin-1β protects astrocytes against oxidant-induced injury via an NF-κB-dependent upregulation of glutathione synthesis[J]. Glia, 2015, 63(9): 1568-1580.
[30]	MENDOZA E E, BURD R. Quercetin as a systemic chemopreventative agent: structural and functional mechanisms[J]. Mini Rev Med Chem, 2011, 11(14): 1216-1221.
[31]	SUN D T, TENG N, YOU Y, et al. Effects of dietary quercetin on production performance and antioxidation in laying hens[J]. China Feed, 2014(21): 8-10, 13. (in Chinese)孙丹彤, 滕楠, 由莹, 等. 日粮添加槲皮素对蛋鸡生产性能及抗氧化能力的影响[J]. 中国饲料, 2014, (21): 8-10.
[32]	YANG J X, GUO J, YUAN J F. In vitro antioxidant properties of rutin[J]. LWT-Food Sci Technol, 2008, 41(6): 1060-1066.
[33]	LI Y P, PANG Q H, QIN H, et al. Effects of Rutin on growth performance, serum biochemical indexes and ascites syndrome of AA broilers[J]. China Poultry, 2019, 41(17): 30-33. (in Chinese)李颖平, 庞全海, 秦红, 等. 芦丁对AA肉鸡生长性能肉鸡生长性能、血清生化指标和肉鸡腹水综合征的影响[J]. 中国家禽, 2019, 41(17): 30-33.
[34]	ZHAN J S, ZHONG X J, YANG Q, et al. Bio-active functions of rutin and its application in ruminant production[J]. Chinese Journal of Animal Nutrition, 2019, 31(7): 2952-2957. (in Chinese)占今舜, 钟小军, 杨群, 等. 芦丁的生物活性功能及其在反刍动物生产中的应用[J]. 动物营养学报, 2019, 31(7): 2952-2957.
[35]	CHEN M, FU G X, DONG J E, et al. Effects of rutin on growth performance, body composition, non-specific immunity and intestinal digestive enzyme activities of grass carp (Ctenopharyngodon idella)[J]. Chinese Journal of Animal Nutrition, 2019, 31(10): 4868-4876. (in Chinese)陈密, 付国香, 董娟娥, 等. 芦丁对草鱼生长性能、体成分、非特异性免疫力和肠道消化酶活性的影响[J]. 动物营养学报, 2019, 31(10): 4868-4876.
[36]	SAW C L L, GUO Y, YANG A Y, et al. The berry constituents quercetin, kaempferol, and pterostilbene synergistically attenuate reactive oxygen species: involvement of the Nrf2-ARE signaling pathway[J]. Food Chem Toxicol, 2014, 72: 303-311.
[37]	WANG C Q, WANG J Y, HUANG B S, et al. Effects of dietary arachidonic acid level on growth performance, antioxidant ability, serum biochemical parameters and fatty acid composition in liver and muscle of juvenile hybrid grouper (Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂)[J]. Chinese Journal of Animal Nutrition, 2018, 30(9): 3567-3580. (in Chinese)王成强, 王际英, 黄炳山, 等. 饲料花生四烯酸水平对珍珠龙胆石斑鱼幼鱼生长性能、抗氧化能力、血清生化指标以及肝脏和肌肉脂肪酸组成的影响[J]. 动物营养学报, 2018, 30(9): 3567-3580.
[38]	CHENG Y F, CHEN Y P, LI J, et al. Dietary β-sitosterol regulates serum lipid level and improves immune function, antioxidant status, and intestinal morphology in broilers[J]. Poult Sci, 2020, 99(3): 1400-1408.
[39]	YAO Y N. The application of β-carotene in livestock and poultry production[J]. China Feed Additive, 2016(3): 20-22. (in Chinese)姚玉妮. β-胡萝卜素在畜禽生产中的应用[J]. 中国饲料添加剂, 2016(3): 20-22.
[40]	WANG Y, LIU S, DUAN J C, et al. Effect of β-carotene addition in feed on growth and antioxidant enzyme activities of Exopalaemon carinicauda[J]. Marine Fisheries, 2020, 42(4): 427-434. (in Chinese)王玉, 柳森, 段健诚, 等. 饲料中β-胡萝卜素添加量对脊尾白虾生长及抗氧化酶活性的影响[J]. 海洋渔业, 2020, 42(4): 427-434.
[41]	FENG H S, YAO H T, WANG Z K, et al. Effects of beta-carotene and astaxanthin from fermentation on egg quality and antioxidant capacity in laying hens[J]. Contemporary Animal Husbandry, 2020(1): 14-18. (in Chinese)封海生, 姚红涛, 王志宽, 等. 发酵来源β-胡萝卜素和虾青素对蛋鸡产蛋品质与抗氧化功能的影响[J]. 当代畜牧, 2020(1): 14-18.
[42]	HUSSAIN T, TAN B, YIN Y L, et al. Oxidative stress and inflammation: what polyphenols can do for us?[J]. Oxid Med Cell Longev, 2016, 2016: 7432797.
[43]	PLATNICH J M, MURUVE D A. NOD-like receptors and inflammasomes: A review of their canonical and non-canonical signaling pathways[J]. Arch Biochem Biophys, 2019, 670: 4-14.
[44]	SATOH T, AKIRA S. Toll-like receptor signaling and its inducible proteins[J]. Microbiol Spectr, 2016, 4(6), doi:10.1128/microbiolspec.MCHD-0040-2016.
[45]	DEVCHAND P R, ZIOUZENKOVA O, PLUTZKY J. Oxidative stress and peroxisome proliferator-activated receptors: reversing the curse?[J]. Circ Res, 2004, 95(12): 1137-1139.
[46]	MORAN E, DING L X, WANG Z X, et al. Protective and antioxidant effects of PPARα in the ischemic retina[J]. Invest Ophthalmol Vis Sci, 2014, 55(7): 4568-4576.
[47]	RADI E, FORMICHI P, BATTISTI C, et al. Apoptosis and oxidative stress in neurodegenerative diseases[J]. J Alzheimers Dis, 2014, 42(S3): S125-S152.
[48]	XU T, DING W, JI X Y, et al. Oxidative stress in cell death and cardiovascular diseases[J]. Oxid Med Cell Longev, 2019, 2019: 9030563.

Mechanism of Mori Folium in Improving Antioxidative Function of Chicken Based on Network Pharmacology

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