母猪添加益生菌和合生元对子代巴马香猪肌肉脂肪酸组成及相关基因表达的影响

doi:10.11843/j.issn.0366-6964.2023.06.024

Abstract

Abstract: The present study was aimed to determine the effects of maternal addition with probiotics and synbiotics on muscular fatty acid composition and related gene expression of offspring. A total of 64 pregnant Bama mini-pigs were selected and randomly divided into control (antibiotic-free basal diet), antibiotic (50 g·t^-1 virginiamycin), probiotics (200 mL·d^-1 probiotic mixture), and synbiotics (500 g·t^-1 xylo-oligosaccharide + 200 mL·d^-1 probiotics mixture) groups. The sows were fed with their corresponding experimental diets during pregnancy and lactation. After weaning, two piglets per litter (32 piglets per group) were selected and fed with a basal diet. At 125 days of age, 8 pigs per group were selected and their biceps femoris (BF) and psoas major (PM) muscles were sampled to determine medium- and long-chain fatty acid composition and related gene expressions. Results showed that, compared with the control group, the C20:2 content in BF muscle was significantly increased (P<0.05) and the expression of stearyl coenzyme A desaturase (SCD) and sterol-regulatory element binding protein-1 (SREBP-1) in BF muscle were significantly up-regulated (P<0.05) in the antibiotic group. In the probiotics group, the C17:0 content in BF and PM muscles were significantly decreased (P<0.05), and the expression of hormone sensitive lipase (HSL) in BF muscle and peroxisome proliferator-activated receptor γ (PPARγ) in PM muscle were up-regulated (P<0.05). In the synbiotics group, the content of C18:2n6c, C20:1, and n-6 PUFA in BF muscle were significantly decreased (P<0.05), and the expression of PPARα in PM muscle was significantly up-regulated (P<0.05). In the antibiotic and probiotics groups, the C20:0 content of BF muscle was significantly decreased (P<0.05), while the expression of PPARα in BF muscle, triglyceride lipase (ATGL) and fatty acid binding protein 4 (FABP4) in PM muscle were significantly up-regulated (P<0.05). The expression of carnitine palmityl transferase 1 (CPT-1) and lipoprotein lipase (LPL) in PM muscle were significantly up-regulated (P<0.05) in the probiotics and synbiotics groups. In the antibiotic, probiotics and synbiotics groups, the C18:1n9t content in BF muscle was significantly decreased (P<0.05), and the expression of ATGL, FABP4, and LPL in BF muscle were up-regulated (P<0.05). In conclusion, maternal addition with probiotics and synbiotics could alter offspring’s muscular fatty acid composition by regulating the expression of genes related to lipid metabolism, which are beneficial to improve the nutrition value and flavor of meat. In addition, maternal antibiotic addition could improve offspring’s muscular fatty acid composition.

Key words: Bama mini-pigs, probiotics, synbiotics, medium- and long-chain fatty acid, lipid metabolism

CLC Number:

S828.91

ZHU Qian, CHENG Yating, LI Ruixuan, LI Chenjian, LIU Yating, KONG Xiangfeng. Effects of Probiotics and Synbiotics Addition to Sows’ Diet on Fatty Acid Composition and Related Gene Expression in Muscle of Offspring Bama Mini-Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2458-2467.

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Effects of Probiotics and Synbiotics Addition to Sows’ Diet on Fatty Acid Composition and Related Gene Expression in Muscle of Offspring Bama Mini-Pigs

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