基于LCMS技术对牦牛围产后期的血清动态代谢组学分析

doi:10.11843/j.issn.0366-6964.2022.11.009

Abstract

Abstract: The purpose of this study was to elucidate the dynamic metabolism of parous yaks in the late perinatal period and explore the mechanism of nutritional metabolism affecting reproductive hormones by metabolomics. Eight parous female yaks with similar age ((7.13±0.78)years), body condition score (2.69±0.35) and parity (1.75±0.43) were randomly selected. Serum was collected every 7 d from the date of the calving day to the 28th days after calving, in total 5 time points. The serum samples at 5 time points were detected by liquid chromatography mass spectrometry, and the key metabolites were screened by bioinformatics analysis to construct the dynamic metabolic network mechanism of parous yaks in the late perinatal period. The qualitative analysis results of 2 841 and 1 326 metabolites were obtained in the positive and negative ion modes, respectively. A total of 117 differential metabolites were screened by calculating the fold change of metabolite expression at each time point, VIP value and P value of T-test at each two time points. According to bioinformatics analysis, 3, 7 and 8 key metabolites related to reproductive performance, lipid metabolism and amino acid metabolism were obtained. The mechanism of constructing dynamic metabolic network based on key metabolites found that the initial efficiency of glucose metabolism in the late perinatal period was low and increased in the late perinatal period, while the efficiency of lipid metabolism and amino acid metabolism was high at the beginning and decreased in the late, it was speculated that the negative energy balance of parous female yaks occurred. The efficiency of reproductive hormone synthesis and secretion remained at a low level in the late perinatal period. According to the detection of dynamic metabolic spectrum and the construction of network mechanism of parous female yaks, it is found that there is a negative energy balance after calving, and the synthesis and secretion of estradiol are blocked due to excessive fat mobilization, which may be the main reason for the failure of postpartum reproductive function of parous female yaks to recover in time, but the specific mechanism needs to be further verified.

Key words: yaks, perinatal period, metabolomics, reproduction, liquid phase mass spectrometry

CLC Number:

S823.85

MA Xiaoling, PENG Wei, SHU Shi. Serum Dynamic Metabonomics Analysis of Yaks in Late Perinatal Period Based on LCMS Technology[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 3811-3826.

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Serum Dynamic Metabonomics Analysis of Yaks in Late Perinatal Period Based on LCMS Technology

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