FecB基因在绵羊繁殖性能上的研究进展

doi:10.11843/j.issn.0366-6964.2023.04.003

Abstract

Abstract: The reproductive performance of sheep is a very important economic trait. The number of lambs is directly related to the number of ovulation each time, and the growth and development of ovum is influenced by the interaction of ovarian endocrine and paracrine. FecB gene is the first multifetal major gene discovered in sheep. The BMP signaling pathway in the ovary of sheep carrying this gene is damaged and the activity is reduced, and some members of TGF-β family are inactivated, which inhibits the proliferation of GCs and improves the sensitivity of FSH, effectively improves ovulation rate and lambing rate of sheep, which is conducive to expanding the breeding scale and improving economic benefits. In this paper, the discovery of FecB gene, its action mechanism, its effect on follicular development, reproductive ability and offspring growth and development, as well as its application in breeding were reviewed, in order to provide reference for the mechanism research and breeding work of improving sheep reproductive performance.

Key words: sheep, FecB, reproductive performance

CLC Number:

S826.3

YANG Chulei, ZHANG Yiyuan, TANG Hong, GUO Yanhua, REN Xiumeiao, WANG Limin, ZHOU Ping. Research Progress of FecB Gene on Reproductive Performance of Sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1370-1380.

References 76

[1]	吴翠玲, 赵卓, 赵云辉, 等.母羊多胎基因研究进展[J].黑龙江畜牧兽医, 2018(11):68-71.WU C L, ZHAO Z, ZHAO Y H, et al.Research progress of fecundity genes in ewe[J].Heilongjiang Animal Science and Veterinary Medicine, 2018(11):68-71.(in Chinese)
[2]	南景东, 陈国旺, 张建胜, 等.绵羊多胎基因研究现状[J].现代畜牧兽医, 2022(11):75-78.NAN J D, CHEN G W, ZHANG J S, et al.Research status of prolificacy gene in sheep[J].Modern Journal of Animal Husbandry and Veterinary Medicine, 2022(11):75-78.(in Chinese)
[3]	PIPER L R, BINDON B M.The Booroola Merino and the performance of medium Non-Peppin crosses at Armidale[J].Wool Technol Sheep Breed, 1982, 31(1):14-19, 33.
[4]	DAVIS G H, MONTGOMERY G W, ALLISON A J, et al.Segregation of a major gene influencing fecundity in progeny of Booroola sheep[J].New Zealand J Agric Res, 1982, 25(4):525-529.
[5]	MULSANT P, LECERF F, FABRE S, et al.Mutation in bone morphogenetic protein receptor-IB is associated with increased ovulation rate in Booroola Mérino ewes[J].Proc Natl Acad Sci U S A, 2001, 98(9):5104-5109.
[6]	WILSON T, WU X Y, JUENGEL J L, et al.Highly prolific Booroola sheep have a mutation in the intracellular kinase domain of bone morphogenetic protein IB receptor (ALK-6) that is expressed in both oocytes and granulosa cells[J].Biol Reprod, 2001, 64(4):1225-1235.
[7]	SOUZA C J, MACDOUGALL C, CAMPBELL B, et al.The Booroola (FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type 1 B (BMPR1B) gene[J].J Endocrinol, 2001, 169(2):R1-R6.
[8]	郭晓飞.FecB基因影响小尾寒羊繁殖力的分子机制研究[D].北京:中国农业大学, 2018.GUO X F.Study on molecular mechanism of FecB gene for fecundity in small tail Han sheep[D].Beijing:China Agricultural University, 2018.(in Chinese)
[9]	QI M Y, XU L Q, ZHANG J N, et al.Effect of the Booroola fecundity (FecB) gene on the reproductive performance of ewes under assisted reproduction[J].Theriogenology, 2020, 142:246-250.
[10]	MCNATTY K P, HEATH D A, CLARK Z, et al.Ovarian characteristics in sheep with multiple fecundity genes[J].Reproduction, 2017, 153(2):233-240.
[11]	王雨曈.不同BMPR-IB基因型对绵羊初情期生殖激素及生长发育的影响[D].石河子:石河子大学, 2020.WANG Y T.Effects of different BMPR-IB genotypes on reproductive hormones, growth and development in puberty of sheep[D]. Shihezi:Shihezi University, 2020.(in Chinese)
[12]	DAVIS G H, GALLOWAY S M, ROSS I K, et al.DNA tests in prolific sheep from eight countries provide new evidence on origin of the Booroola (FecB) mutation[J].Biol Reprod, 2002, 66(6):1869-1874.
[13]	KUMAR S, MISHRA A K, KOLTE A P, et al.Screening for Booroola (FecB) and Galway (FecXG) mutations in Indian sheep[J]. Small Rumin Res, 2008, 80(1-3):57-61.
[14]	ROY J, POLLEY S, DE S, et al.Polymorphism of fecundity genes (FecB, FecX, and FecG) in the Indian Bonpala sheep[J].Anim Biotechnol, 2011, 22(3):151-162.
[15]	ORAON T, SINGH D K, GHOSH M, et al.Allelic and genotypic frequencies in polymorphic Booroola fecundity gene and their association with multiple birth and postnatal growth in Chhotanagpuri sheep[J].Vet World, 2016, 9(11):1294-1299.
[16]	MAHDAVI M, NANEKARANI S, HOSSEINI S D.Mutation in BMPR-IB gene is associated with litter size in Iranian Kalehkoohi sheep[J].Anim Reprod Sci, 2014, 147(3-4):93-98.
[17]	WANG X Y, GUO X F, HE X Y, et al.Effects of FecB mutation on estrus, ovulation, and endocrine characteristics in Small Tail Han sheep[J].Front Vet Sci, 2021, 8:709737.
[18]	喇永富, 李发弟, 杨勤, 等.FecB基因在5个中国地方绵羊品种中的多态性及其与产羔数的关联分析[J].中国草食动物科学, 2020, 40(2):12-17.LA Y F, LI F D, YANG Q, et al.Genetic polymorphism of FecB gene and effect in five Chinese local sheep breeds[J].China Herbivore Science, 2020, 40(2):12-17.(in Chinese)
[19]	杨华, 杨永林, 刘守仁, 等.绵羊BMPR-IB基因单核苷酸多态性分析[J].西北农业学报, 2010, 19(9):7-11.YANG H, YANG Y L, LIU S R, et al.Analysis of single nucleotide polymorphism of BMPR-IB gene in sheep[J].Acta Agriculturae Boreali-Occidentalis Sinica, 2010, 19(9):7-11.(in Chinese)
[20]	柏雪梅, 薛亚欣, 邢凤, 等.策勒黑羊和多浪羊FecB基因多态性及其与产羔数的相关性研究[J].黑龙江畜牧兽医, 2020(1):7-10.BAI X M, XUE Y X, XING F, et al.Association of FecB gene polymorphism with litter size in Cele Black sheep and Duolang sheep[J].Heilongjiang Animal Science and Veterinary Medicine, 2020(1):7-10.(in Chinese)
[21]	牛志刚, 王珊, 阿克达尔, 等.洼地绵羊FecB基因多态性及与产羔数的相关性[J].黑龙江畜牧兽医, 2016(13):103-105.NIU Z G, WANG S, AKEDAER, et al.Polymorphism of FecB gene and its correlation with litter size in Wadi sheep[J].Heilongjiang Animal Science and Veterinary Medicine, 2016(13):103-105.(in Chinese)
[22]	王勇, 马丽娜, 梁小军.绵羊FecB基因的基因分型检测[J].宁夏农林科技, 2020, 61(10):43-44, 51.WANG Y, MA L N, LIANG X J.The detection genotyping of FecB gene in sheep[J].Ningxia Journal of Agriculture and Forestry Science and Technology, 2020, 61(10):43-44, 51.(in Chinese)
[23]	ZUO B Y, QIAN H G, WANG Z Y, et al.A study on BMPR-IB genes of Bayanbulak sheep[J].Asian-Australas J Anim Sci, 2013, 26(1):36-42.
[24]	王琼, 马海玉, 刘玲玲, 等.GDF9、BMPR1B与BMP15基因在和田羊群体的遗传特征分析[J].草食家畜, 2020(4):1-8.WANG Q, MA H Y, LIU L L, et al.Analysis on the genetic characteristics of GDF9, BMPR1B and BMP15 gene in Hotan sheep population[J].Grass-Feeding Livestock, 2020(4):1-8.(in Chinese)
[25]	杨华, 陈新华, 李爱芳, 等.多胎主效基因在绵羊育种和生产实践中的应用[J].新疆农垦科技, 2010, 33(3):42-43.YANG H, CHEN X H, LI A F, et al.Application of multiple major genes in sheep breeding and production practice[J].Xinjiang Farm Research of Science and Technology, 2010, 33(3):42-43.(in Chinese)
[26]	郭立宏.应用SSCP标记对东北细毛羊多胎基因(FecB)型检测的研究[J].黑龙江畜牧兽医, 2017(5):95-97.GUO L H.Study on the detection of multiple fetal gene (FecB) type by SSCP marker in Northeast fine wool sheep[J].Heilongjiang Animal Science and Veterinary Medicine, 2017(5):95-97.(in Chinese)
[27]	张也, 艾日夏提·地里夏提, 刘志强, 等.FecB基因对杜泊羊和湖羊杂交后代产羔率与生长性能的影响[J].中国草食动物科学, 2021, 41(2):69-72.ZHANG Y, AIRIXIATI·DILIXIATI, LIU Z Q, et al.Effect of FecB gene on lambing percentage and growth performance of cross offspring of Doper and Hu sheep[J].China Herbivore Science, 2021, 41(2):69-72.(in Chinese)
[28]	马丽娜, 于洋.滩羊多胎基因(FecB)TaqMan探针SNP分型与产羔数的关联研究[J].畜牧与饲料科学, 2017, 38(3):20-22.MA L N, YU Y.Correlation analysis between fecundity gene (FecB) SNP genotyping by TaqMan probe and litter size in Tan sheep[J].Animal Husbandry and Feed Science, 2017, 38(3):20-22.(in Chinese)
[29]	MCNATTY K P, HUDSON N L, LUN S, et al.Gonadotrophin-releasing hormone and the control of ovulation rate by the FecB gene in Booroola ewes[J].J Reprod Fertil, 1993, 98(1):97-105.
[30]	ISAACS K L, MCNATTY K P, CONDELL L, et al.Plasma FSH, LH and immunoreactive inhibin concentrations in FecBB/FecBB and FecB+/FecB+ Booroola ewes and rams from birth to 12 months of age[J].J Reprod Fertil, 1995, 103(1):89-97.
[31]	周明亮, 庞倩, 陈明华, 等.我国绵羊FecB基因的研究进展[J].草学, 2021(4):68-72.ZHOU M L, PANG Q, CHEN M H, et al.Research progress of FecB gene in sheep in China[J].Journal of Grassland and Forage Science, 2021(4):68-72.(in Chinese)
[32]	AKHATAYEVA Z, CAO C N, HUANG Y M, et al.Newly reported 90-bp deletion within the ovine BMPRIB gene:Does it widely distribute, link to the famous FecB (p.Q249R) mutation, and affect litter size?[J].Theriogenology, 2022, 189:222-229.
[33]	陶林, 郭晓飞, 文禹粱, 等.FecB突变对小尾寒羊卵泡发育及AMH和AMHR2基因表达的影响[J].农业生物技术学报, 2019, 27(4):684-691.TAO L, GUO X F, WEN Y L, et al.Effects of FecB mutation on follicular development and expression of AMH and AMHR2 genes in Small Tail Han sheep (Ovis aries)[J].Journal of Agricultural Biotechnology, 2019, 27(4):684-691.(in Chinese)
[34]	ESTIENNE A, PIERRE A, DI CLEMENTE N, et al.Anti-Müllerian hormone regulation by the bone morphogenetic proteins in the sheep ovary:deciphering a direct regulatory pathway[J].Endocrinology, 2015, 156(1):301-313.
[35]	MAZERBOURG S, BONDY C A, ZHOU J, et al.The insulin-like growth factor system:a key determinant role in the growth and selection of ovarian follicles?A comparative species study[J].Reprod Domest Anim, 2003, 38(4):247-258.
[36]	JUENGEL J L, MCNATTY K P.The role of proteins of the transforming growth factor-β superfamily in the intraovarian regulation of follicular development[J].Hum Reprod Update, 2005, 11(2):143-160.
[37]	GOYAL S, AGGARWAL J, DUBEY P K, et al.Expression analysis of genes associated with prolificacy in FecB carrier and noncarrier Indian sheep[J].Anim Biotechnol, 2017, 28(3):220-227.
[38]	TIAN D H, LIU S J, TIAN F, et al.Comparative transcriptome of reproductive axis in Chinese indigenous sheep with different FecB genotypes and prolificacies[J].Anim Reprod Sci, 2020, 223:106624.
[39]	王雪, 张景萍, 郭丽丽, 等.TGF-β/SMAD信号通路在哺乳动物卵巢发育调控中的作用研究进展[J].中国畜牧杂志, 2023, 59(3):61-69.WANG X, ZHANG J P, GUO L L, et al.Research progress on the regulation of TGF-β/Smad signaling pathway in mammalian ovarian development[J].Chinese Journal of Animal Science, 2023, 59(3):61-69.(in Chinese)
[40]	FABRE S, PIERRE A, MULSANT P, et al.Regulation of ovulation rate in mammals:contribution of sheep genetic models[J]. Reprod Biol Endocrinol, 2006, 4:20.
[41]	CLARKE H J.Regulation of germ cell development by intercellular signaling in the mammalian ovarian follicle[J].WIREs Dev Biol, 2018, 7(1):e294.
[42]	TONG B, WANG J P, CHENG Z X, et al.Novel variants in GDF9 gene affect promoter activity and litter size in Mongolia sheep[J].Genes (Basel), 2020, 11(4):375.
[43]	D'OCCHIO M J, CAMPANILE G, BARUSELLI P S.Transforming growth factor-β superfamily and interferon-τ in ovarian function and embryo development in female cattle:review of biology and application[J].Reprod Fertil Dev, 2020, 32(6):539-552.
[44]	晁哲, 邢漫萍, 黄丽丽, 等.海南黑山羊GDF9和BMP15基因多态性与初胎产羔数间的关系[J].畜牧与兽医, 2021, 53(11):14-20.CHAO Z, XING M P, HUANG L L, et al.Correlation between genetic polymorphisms of the GDF9 and BMP15 genes and the litter size of the first parity of Hainan black goats[J].Animal Husbandry & Veterinary Medicine, 2021, 53(11):14-20.(in Chinese)
[45]	GARCIA-GUERRA A, WILTBANK M C, BATTISTA S E, et al.Mechanisms regulating follicle selection in ruminants:lessons learned from multiple ovulation models[J].Anim Reprod, 2018, 15(S1):660-679.
[46]	LI Q L, AGNO J E, EDSON M A, et al.Transforming growth factor β receptor type 1 is essential for female reproductive tract integrity and function[J].PLoS Genet, 2011, 7(10):e1002320.
[47]	PENG J, LI Q L, WIGGLESWORTH K, et al.Reply to Mottershead et al.:GDF9:BMP15 heterodimers are potent regulators of ovarian functions[J].Proc Natl Acad Sci U S A, 2013, 110(25):E2258.
[48]	张筱艳.绵羊BMPR1B基因SNP和血液BMPR1B蛋白与繁殖性能的相关性研究[D].兰州:甘肃农业大学, 2020.ZHANG X Y.Study of the effects of BMPR1B gene SNP and the quantity of BMPR1B protein in blood on reproductive performance of sheep[D].Lanzhou:Gansu Agricultural University, 2020.(in Chinese)
[49]	MCNATTY K P, JUENGEL J L, READER K L, et al.Bone morphogenetic protein 15 and growth differentiation factor 9 co-operate to regulate granulosa cell function[J].Reproduction, 2005, 129(4):473-480.
[50]	赵金.BMP15通过BMPR1B调控陕北白绒山羊卵泡颗粒细胞功能的分子机制[D].杨凌:西北农林科技大学, 2021.ZHAO J.The molecular mechanism of BMP15 regulates the function of follicular granulosa cells through BMPR1B in Shanbei White Cashmere Goat[D].Yangling:Northwest A&F University, 2021.(in Chinese)
[51]	SAWYER H R, SMITH P, HEATH D A, et al.Formation of ovarian follicles during fetal development in sheep[J].Biol Reprod, 2002, 66(4):1134-1150.
[52]	MCNATTY K P, JUENGEL J L, PITMAN J L.Oocyte-Somatic cell interactions and ovulation rate:effects on oocyte quality and embryo yield[J].Reprod Biol Insights, 2014, 7:1-8.
[53]	BAIRD D T, CAMPBELL B K.Follicle selection in sheep with breed differences in ovulation rate[J].Mol Cell Endocrinol, 1998, 145(1-2):89-95.
[54]	CAMPBELL B K, BAIRD D T, SOUZA C J, et al.The FecB (Booroola) gene acts at the ovary:in vivo evidence[J]. Reproduction, 2003, 126(1):101-111.
[55]	FABRE S, PIERRE A, MULSANT P, et al.Regulation of ovulation rate in mammals:contribution of sheep genetic models[J]. Reprod Biol Endocrinol, 2006, 4:20.
[56]	BELLI M, SHIMASAKI S.Molecular aspects and clinical relevance of GDF9 and BMP15 in ovarian function[J].Vitam Horm, 2018, 107:317-348.
[57]	STANKIEWICZ T, BŁASZCZYK B.Relationship between the concentration of bone morphogenetic protein-15(BMP-15) and growth differentiation factor-9(GDF-9) in pre-ovulatory follicles, ovarian cysts and serum in sows[J].Anim Prod Sci, 2016, 56(1):141-146.
[58]	种玉晴.绵羊产羔数性状的分子遗传机理研究[D].武汉:华中农业大学, 2021.ZHONG Y Q.Study on molecular genetic mechanism of litter size in sheep[D].Wuhan:Huazhong Agricultural University, 2021.(in Chinese)
[59]	郑宇新, 张妮娜, 崔亚娟, 等.雌激素受体在卵泡发育中作用的研究进展[J].黑龙江畜牧兽医, 2018(1):60-62.ZHENG Y X, ZHANG N N, CUI Y J, et al.Progress of estrogen receptors effect on follicular development[J].Heilongjiang Animal Science and Veterinary Medicine, 2018(1):60-62.(in Chinese)
[60]	LIU W, XIN Q L, WANG X, et al.Estrogen receptors in granulosa cells govern meiotic resumption of pre-ovulatory oocytes in mammals[J].Cell Death Dis, 2017, 8(3):e2662.
[61]	郭晓飞, 王翔宇, 张金龙, 等.FecB突变对绵羊发情表型及格拉夫卵泡颗粒细胞中ESR1和ESR2基因表达的影响[J].黑龙江畜牧兽医, 2020(9):39-43.GUO X F, WANG X Y, ZHANG J L, et al.Effect of FecB mutation on ovine estrus and expression of ESR1 and ESR2 genes in Graafian follicle granule cells in sheep[J].Heilongjiang Animal Science and Veterinary Medicine, 2020(9):39-43.(in Chinese)
[62]	READER K L, HAYDON L J, LITTLEJOHN R P, et al.Booroola BMPR1B mutation alters early follicular development and oocyte ultrastructure in sheep[J].Reprod Fertil Dev, 2012, 24(2):353-361.
[63]	JUENGEL J L, FRENCH M C, QUIRKE L D, et al.Differential expression of CART in ewes with differing ovulation rates[J]. Reproduction, 2017, 153(4):471-479.
[64]	MCNATTY K P, HEATH D A, HUDSON N L, et al.Gonadotrophin-responsiveness of granulosa cells from bone morphogenetic protein 15 heterozygous mutant sheep[J].Reproduction, 2009, 138(3):545-551.
[65]	KUMAR S, RAJPUT P K, BAHIRE S V, et al.Differential expression of BMP/SMAD signaling and ovarian-associated genes in the granulosa cells of FecB introgressed GMM sheep[J].Syst Biol Reprod Med, 2020, 66(3):185-201.
[66]	崔绪奎, 王金文, 王德芹, 等.FecB基因对杜×寒杂交肉羊产羔数及羔羊生长发育的影响[J].山东农业科学, 2010(8):98-100+106.CUI X K, WANG J W, WANG D Q, et al. Effect of FecB gene on litter sice and lamb growth and development of Dorper×Han crossbred sheep[J]. Shandong Agricultural Sciences, 2010(8):98-100+106.(in Chinese)
[67]	SEJIAN V, MAURYA V P, PRINCE L L L, et al.Effect of FecB status on the allometric measurements and reproductive performance of Garole×Malpura ewes under hot semi-arid environment[J].Trop Anim Health Prod, 2015, 47(6):1089-1093.
[68]	CHU M X, LIU Z H, JIAO C L, et al.Mutations in BMPR-IB and BMP-15 genes are associated with litter size in Small Tailed Han sheep (Ovis aries)[J].J Anim Sci, 2007, 85(3):598-603.
[69]	王伟霞, 佟桂芝, 刘学峰, 等.多胎基因FecB对德×寒×细三元杂交羊产羔性能的影响[J].畜牧与饲料科学, 2020, 41(4):32-35, 59.WANG W X, TONG G Z, LIU X F, et al.Effect of FecB gene on lambing performance of Germany Mutton Merino sheep×Small-tailed Han sheep×Fine-wool Sheep ternary hybrid sheep[J].Animal Husbandry and Feed Science, 2020, 41(4):32-35, 59.(in Chinese)
[70]	罗生金.利用FecB多胎基因导入培育哈萨克羊多羔类型的研究[J].黑龙江动物繁殖, 2017, 25(2):7-10, 12.LUO S J.Study on Breeding multiple lambs type of Kazakh sheep by FecB polyfetal gene introduction[J].Heilongjiang Journal of Animal Reproduction, 2017, 25(2):7-10, 12.(in Chinese)
[71]	于洋, 梁小军, 马吉锋, 等.滩湖F2代FecB基因的多态性检测[J].上海畜牧兽医通讯, 2018(4):44-45.YU Y, LIANG X J, MA J F, et al.Polymorphism detection of FecB gene in the F2 generation of Hybrid Tan sheep and Hu sheep[J]. Shanghai Journal of Animal Husbandry and Veterinary Medicine, 2018(4):44-45.(in Chinese)
[72]	ZHOU S W, DING Y G, LIU J, et al.Highly efficient generation of sheep with a defined FecBB mutation via adenine base editing[J]. Genet Sel Evol, 2020, 52(1):35.
[73]	于振兴, 贺志锐, 吾热力哈孜, 等.湖羊FecB基因在新疆细毛羊胎儿成纤维细胞中的表达[J].动物医学进展, 2012, 33(6):99-102.YU Z X, HE Z R, WURELI H Z, et al.Expression of Hu sheep FecB gene in fetal fibroblasts of Xinjiang Fine-wool sheep[J].Progress in Veterinary Medicine, 2012, 33(6):99-102.(in Chinese)
[74]	NAGDY H, MAHMOUD K G M, KANDIEL M M M, et al.PCR-RFLP of bone morphogenetic protein 15(BMP15/FecX) gene as a candidate for prolificacy in sheep[J].Int J Vet Sci Med, 2018, 6(S1):S68-S72.
[75]	TONG B, WANG J P, CHENG Z X, et al.Novel variants in GDF9 gene affect promoter activity and litter size in Mongolia sheep[J].Genes (Basel), 2020, 11(4):375.
[76]	梁永厚, 王贵, 梅步俊.绵羊多胎基因研究进展[J].当代畜牧, 2018(36):17-23.LIANG Y H, WANG G, MEI B J.Research progress of sheep Poly-embryonal gene[J].Contemporary Animal Husbandry, 2018(36):17-23.(in Chinese)

Research Progress of FecB Gene on Reproductive Performance of Sheep

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