OPU卵母细胞体外成熟体系的优化研究进展

doi:10.11843/j.issn.0366-6964.2023.04.002

Abstract

Abstract: Recent years, techniques for in vitro embryo production (IVP) have advanced significantly, especially the ovum pick-up (OPU) technology grows widely. OPU is a new technology developed in the 1990 s that can obtain oocytes from excellent breeding heifers, which has a broad application prospect in the research of the mechanism of animal embryo production and development and in the clinical practice of human assisted reproductive technology. It can improve the resource protection efficiency of breeding females and precious wild animals, and the genetic potential of excellent females, and provide the necessary supporting technology for the development of cattle biotechnology. The quality of oocytes is the key to the success of the IVP system, and the in vitro maturation (IVM) process of the oocytes is critical for successful fertilization and embryo development. Oocyte IVM completes the cytoplasmic and nuclear maturation of the oocyte, preparing all necessary components for development to the activation of the embryonic genome. Developmental ability of oocytes matured in vitro is lower than that of oocytes matured in vivo, non-synchronization of oocyte meiosis and nucleocytoplasmic maturation, oxidative stress damage, et al are important for successful fertilization and embryo development. Therefore, it is necessary to further improve the in vitro maturation ability of oocytes to improve the bovine IVP system. In this paper, the problems of in vitro maturation of oocytes such as non-synchronization of nucleocytoplasmic maturation and oxidative stress were summarized, as well as the improvement effects of C-type natriuretic peptide, melatonin, FLI and other substances on oocytes IVM were discussed in order to improve the bovine IVP system.

Key words: OPU, oocyte, in vitro maturation, bovine

CLC Number:

S814.6

ZHANG Peipei, HAO Haisheng, DU Weihua, ZHU Huabin, LI Shujing, YU Wenli, ZHAO Xueming. A Review of Optimization of in vitro Maturation System of OPU Oocytes[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1359-1369.

References 100

[1]	BALBOULA A Z, ABOELENAIN M, SAKATANI M, et al.Effect of E-64 supplementation during in vitro maturation on the developmental competence of bovine OPU-derived oocytes[J].Genes (Basel), 2022, 13(2):324.
[2]	FERRÉ L B, KJELLAND M E, TAIYEB A M, et al.Recent progress in bovine in vitro-derived embryo cryotolerance:Impact of in vitro culture systems, advances in cryopreservation and future considerations[J].Reprod Domest Anim, 2020, 55(6):659-676.
[3]	NIEMANN H, KUES W A.Application of transgenesis in livestock for agriculture and biomedicine[J].Anim Reprod Sci, 2003, 79(3-4):291-317.
[4]	HASLER J F.The current status and future of commercial embryo transfer in cattle[J].Anim Reprod Sci, 2003, 79(3-4):245-264.
[5]	BLONDIN P.Logistics of large scale commercial IVF embryo production[J].Reprod Fertil Dev, 2017, 29(1):32-36.
[6]	PERRY G.Statistics of embryo collection and transfer in domestic farm animals[J].ET Newsletter, 2017, 35:8-23.
[7]	MATOBA S, YOSHIOKA H, MATSUDA H, et al.Optimizing production of in vivo-matured oocytes from superstimulated Holstein cows for in vitro production of embryos using X-sorted sperm[J].J Dairy Sci, 2014, 97(2):743-753.
[8]	JAFFE L A, EGBERT J R.Regulation of mammalian oocyte meiosis by intercellular communication within the ovarian follicle[J]. Annu Rev Physiol, 2017, 79:237-260.
[9]	COTICCHIO G, DAL CANTO M, MIGNINI RENZINI M, et al.Oocyte maturation:gamete-somatic cells interactions, meiotic resumption, cytoskeletal dynamics and cytoplasmic reorganization[J].Hum Reprod Update, 2015, 21(4):427-454.
[10]	PINCUS G, ENZMANN E V.The comparative behavior of mammalian eggs in vivo and in vitro:I.The activation of ovarian eggs[J].J Exp Med, 1935, 62(5):655-675.
[11]	ASSEY R J, HYTTEL P, GREVE T, et al.Oocyte morphology in dominant and subordinate follicles[J].Mol Reprod Dev, 1994, 37(3):335-344.
[12]	MACHATKOVA M, KRAUSOVA K, JOKESOVA E, et al.Developmental competence of bovine oocytes:effects of follicle size and the phase of follicular wave on in vitro embryo production[J].Theriogenology, 2004, 61(2-3):329-335.
[13]	FRANCIOSI F, COTICCHIO G, LODDE V, et al.Natriuretic peptide precursor C delays meiotic resumption and sustains gap junction-mediated communication in bovine cumulus-enclosed oocytes[J].Biol Reprod, 2014, 91(3):61.
[14]	GONG X Q, LI H M, ZHAO Y Q.The improvement and clinical application of human oocyte in vitro maturation (IVM)[J]. Reprod Sci, 2022, 29(8):2127-2135.
[15]	GILCHRIST R B, LUCIANO A M, RICHANI D, et al.Oocyte maturation and quality:role of cyclic nucleotides[J].Reproduction, 2016, 152(5):R143-R157.
[16]	MENG L, WU Z F, ZHAO K, et al.Transcriptome analysis of porcine granulosa cells in healthy and atretic follicles:Role of steroidogenesis and oxidative stress[J].Antioxidants (Basel), 2020, 10(1):22.
[17]	TAKAHASHI M.Oxidative stress and redox regulation on in vitro development of mammalian embryos[J].J Reprod Dev, 2012, 58(1):1-9.
[18]	CAJAS Y N, CAÑÓN-BELTRÁN K, DE GUEVARA M L, et al.Antioxidant nobiletin enhances oocyte maturation and subsequent embryo development and quality[J].Int J Mol Sci, 2020, 21(15):5340.
[19]	WANG L, TANG J H, WANG L, et al.Oxidative stress in oocyte aging and female reproduction[J].J Cell Physiol, 2021, 236(12):7966-7983.
[20]	SUDOH T, MINAMINO N, KANGAWA K, et al.C-type natriuretic peptide (CNP):a new member of natriuretic peptide family identified in porcine brain[J].Biochem Biophys Res Commun, 1990, 168(2):863-870.
[21]	SELLITTI D F, KOLES N, MENDONÇA M C.Regulation of C-type natriuretic peptide expression[J].Peptides, 2011, 32(9):1964-1971.
[22]	STRĄCZYČŃSKA P, PAPIS K, MORAWIEC E, et al.Signaling mechanisms and their regulation during in vivo or in vitro maturation of mammalian oocytes[J].Reprod Biol Endocrinol, 2022, 20(1):37.
[23]	UEDA Y, YASODA A, YAMASHITA Y, et al.C-type natriuretic peptide restores impaired skeletal growth in a murine model of glucocorticoid-induced growth retardation[J].Bone, 2016, 92:157-167.
[24]	NAKAGAWA H, SAITO Y.Roles of natriuretic peptides and the significance of neprilysin in cardiovascular diseases[J].Biology (Basel), 2022, 11(7):1017.
[25]	BOHARA M, KAMBE Y, NAGAYAMA T, et al.C-type natriuretic peptide modulates permeability of the blood-brain barrier[J].J Cereb Blood Flow Metab, 2014, 34(4):589-596.
[26]	HU P, LIU S Y, ZHANG D D, et al.Urinary c-type natriuretic peptide excretion:a promising biomarker to detect underlying renal injury and remodeling both acutely and chronically[J].Biomark Med, 2016, 10(9):999-1008.
[27]	ZHANG M J, SU Y Q, SUGIURA K, et al.Granulosa cell ligand NPPC and its receptor NPR2 maintain meiotic arrest in mouse oocytes[J].Science, 2010, 330(6002):366-369.
[28]	DE SOUSA P A, WATSON A J, SCHULTZ G A, et al.Oogenetic and zygotic gene expression directing early bovine embryogenesis:a review[J].Mol Reprod Dev, 1998, 51(1):112-121.
[29]	MEHLMANN L M, SAEKI Y, TANAKA S, et al.The Gs-linked receptor GPR3 maintains meiotic arrest in mammalian oocytes[J]. Science, 2004, 306(5703):1947-1950.
[30]	HINCKLEY M, VACCARI S, HORNER K, et al.The G-protein-coupled receptors GPR3 and GPR12 are involved in cAMP signaling and maintenance of meiotic arrest in rodent oocytes[J].Dev Biol, 2005, 287(2):249-261.
[31]	FRANCIOSI F, COTICCHIO G, LODDE V, et al.Natriuretic peptide precursor C delays meiotic resumption and sustains gap junction-mediated communication in bovine cumulus-enclosed oocytes[J].Biol Reprod, 2014, 91(3):61.
[32]	SOTO-HERAS S, PARAMIO M T, THOMPSON J G.Effect of pre-maturation with C-type natriuretic peptide and 3-isobutyl-1-methylxanthine on cumulus-oocyte communication and oocyte developmental competence in cattle[J].Anim Reprod Sci, 2019, 202:49-57.
[33]	JIA Z W, YANG X Y, LIU K.Treatment of cattle oocytes with C-type natriuretic peptide before in vitro maturation enhances oocyte mitochondrial function[J].Anim Reprod Sci, 2021, 225:106685.
[34]	XI G Y, AN L, JIA Z W, et al.Natriuretic peptide receptor 2(NPR2) localized in bovine oocyte underlies a unique mechanism for C-type natriuretic peptide (CNP)-induced meiotic arrest[J].Theriogenology, 2018, 106:198-209.
[35]	ZHANG T, FAN X M, LI R L, et al.Effects of pre-incubation with C-type natriuretic peptide on nuclear maturation, mitochondrial behavior, and developmental competence of sheep oocytes[J].Biochem Biophys Res Commun, 2018, 497(1):200-206.
[36]	HORI Y S, KUNO A, HOSODA R, et al.Regulation of FOXOs and p53 by SIRT1 modulators under oxidative stress[J].PLoS One, 2013, 8(9):e73875.
[37]	DI EMIDIO G, FALONE S, VITTI M, et al.SIRT1 signalling protects mouse oocytes against oxidative stress and is deregulated during aging[J].Hum Reprod, 2014, 29(9):2006-2017.
[38]	COMBELLES C M H, GUPTA S, AGARWAL A.Could oxidative stress influence the in-vitro maturation of oocytes?[J].Reprod Biomed Online, 2009, 18(6):864-980.
[39]	NIE J Y, SUI L, ZHANG H T, et al.Mogroside V protects porcine oocytes from in vitro ageing by reducing oxidative stress through SIRT1 upregulation[J].Aging (Albany NY), 2019, 11(19):8362-8373.
[40]	YONG W, MA H Y, NA M, et al.Roles of melatonin in the field of reproductive medicine[J].Biomed Pharmacother, 2021, 144:112001.
[41]	MESALAM A, KHAN I, LEE K L, et al.2-Methoxystypandrone improves in vitro-produced bovine embryo quality through inhibition of IKBKB[J].Theriogenology, 2017, 99:10-20.
[42]	YANG M H, TAO J L, CHAI M L, et al.Melatonin improves the quality of inferior bovine oocytes and promoted their subsequent IVF embryo development:Mechanisms and results[J].Molecules, 2017, 22(12):2059.
[43]	SUN Z Y, ZHANG P, WANG J J, et al.Melatonin alleviates meiotic defects in fetal mouse oocytes induced by Di (2-ethylhexyl) phthalate in vitro[J].Aging (Albany NY), 2018, 10(12):4175-4187.
[44]	YANG F X, LI L, CHEN K L, et al.Melatonin alleviates β-zearalenol and HT-2 toxin-induced apoptosis and oxidative stress in bovine ovarian granulosa cells[J].Environ Toxicol Pharmacol, 2019, 68:52-60.
[45]	REITER R J.Functional pleiotropy of the neurohormone melatonin:Antioxidant protection and neuroendocrine regulation[J]. Front Neuroendocrinol, 1995, 16(4):383-415.
[46]	TAN D X, REITER R J.Mitochondria:The birth place, battle ground and the site of melatonin metabolism in cells[J].Melatonin Res, 2019, 2(1):44-66.
[47]	REITER R J, TAN D X, MANCHESTER L C, et al.Melatonin:Detoxification of oxygen and nitrogen-based toxic reactants[J].Adv Exp Med Biol, 2003, 527:539-548.
[48]	TAN D X, MANCHESTER L C, TERRON M P, et al.One molecule, many derivatives:A never-ending interaction of melatonin with reactive oxygen and nitrogen species?[J].J Pineal Res, 2007, 42(1):28-42.
[49]	MONIRUZZAMAN M, GHOSAL I, DAS D, et al.Melatonin ameliorates H2O2-induced oxidative stress through modulation of Erk/Akt/NFkB pathway[J].Biol Res, 2018, 51(1):17.
[50]	TANABE M, TAMURA H, TAKETANI T, et al.Melatonin protects the integrity of granulosa cells by reducing oxidative stress in nuclei, mitochondria, and plasma membranes in mice[J].J Reprod Dev, 2015, 61(1):35-41.
[51]	TAMURA H, JOZAKI M, TANABE M, et al.Importance of melatonin in assisted reproductive technology and ovarian aging[J].Int J Mol Sci, 2020, 21(3):1135.
[52]	NIU Y J, ZHOU W J, NIE Z W, et al.Melatonin enhances mitochondrial biogenesis and protects against rotenone-induced mitochondrial deficiency in early porcine embryos[J].J Pineal Res, 2020, 68(2):e12627.
[53]	El-RAEY M, GESHI M, SOMFAI T, et al.Evidence of melatonin synthesis in the cumulus oocyte complexes and its role in enhancing oocyte maturation in vitro in cattle[J].Mol Reprod Dev, 2011, 78(4):250-262.
[54]	KHAN H L, BHATTI S, ABBAS S, et al.Melatonin levels and microRNA (miRNA) relative expression profile in the follicular ambient microenvironment in patients undergoing in vitro fertilization process[J].J Assist Reprod Genet, 2021, 38(2):443-459.
[55]	SHI J M, TIAN X Z, ZHOU G B, et al.Melatonin exists in porcine follicular fluid and improves in vitro maturation and parthenogenetic development of porcine oocytes[J].J Pineal Res, 2009, 47(4):318-323.
[56]	ZHU Q, DING D, YANG H, et al.Melatonin protects mitochondrial function and inhibits oxidative damage against the decline of human oocytes development caused by prolonged cryopreservation[J].Cells, 2022, 11(24):4018.
[57]	MANJUNATHA B M, DEVARAJ M, GUPTA P S P, et al.Effect of taurine and melatonin in the culture medium on buffalo in vitro embryo development[J].Reprod Domest Anim, 2009, 44(1):12-16.
[58]	CHATTORAJ A, SETH M, MAITRA S K.Influence of serotonin on the action of melatonin in MIH-induced meiotic resumption in the oocytes of carp Catla catla[J].Comp Biochem Physiol A Mol Integr Physiol, 2008, 150(3):301-306.
[59]	GUTIÉRREZ-AÑEZ J C, LUCAS-HAHN A, HADELER K G, et al.Melatonin enhances in vitro developmental competence of cumulus-oocyte complexes collected by ovum pick-up in prepubertal and adult dairy cattle[J].Theriogenology, 2021, 161:285-293.
[60]	YUAN Y, SPATE L D, REDEL B K, et al.Quadrupling efficiency in production of genetically modified pigs through improved oocyte maturation[J].Proc Natl Acad Sci U S A, 2017, 114(29):E5796-E5804.
[61]	DELAFONTAINE P, SONG Y H, LI Y X.Expression, regulation, and function of IGF-1, IGF-1R, and IGF-1 binding proteins in blood vessels[J].Arterioscler Thromb Vasc Biol, 2004, 24(3):435-444.
[62]	VELAZQUEZ M A, SPICER L J, WATHES D C.The role of endocrine insulin-like growth factor-I (IGF-I) in female bovine reproduction[J].Domest Anim Endocrinol, 2008, 35(4):325-342.
[63]	YU J S L, CUI W.Proliferation, survival and metabolism:the role of PI3K/AKT/mTOR signalling in pluripotency and cell fate determination[J].Development, 2016, 143(17):3050-3060.
[64]	LI X H, DAI Y F, ALLEN W R.Influence of insulin-like growth factor-I on cytoplasmic maturation of horse oocytes in vitro and organization of the first cell cycle following nuclear transfer and parthenogenesis[J].Biol Reprod, 2004, 71(4):1391-1396.
[65]	SIROTKIN A V, DUKESOVÁ J, MAKAREVICH A V, et al.Evidence that growth factors IGF-I, IGF-II and EGF can stimulate nuclear maturation of porcine oocytes via intracellular protein kinase A[J].Reprod Nutr Dev, 2000, 40(6):559-569.
[66]	CARNEIRO G, LORENZO P, PIMENTEL C, et al.Influence of insulin-like growth factor-I and its interaction with gonadotropins, estradiol, and fetal calf serum on in vitro maturation and parthenogenic development in equine oocytes[J].Biol Reprod, 2001, 65(3):899-905.
[67]	WASIELAK M, BOGACKI M.Apoptosis inhibition by insulin-like growth factor (IGF)-I during in vitro maturation of bovine oocytes[J].J Reprod Dev, 2007, 53(2):419-426.
[68]	DEMEESTERE I, GERVY C, CENTNER J, et al.Effect of insulin-like growth factor-I during preantral follicular culture on steroidogenesis, in vitro oocyte maturation, and embryo development in mice[J].Biol Reprod, 2004, 70(6):1664-1669.
[69]	YANG S, YANG Y Z, HAO H S, et al.Supplementation of EGF, IGF-1, and Connexin 37 in IVM medium significantly improved the maturation of bovine oocytes and vitrification of their IVF blastocysts[J].Genes (Basel), 2022, 13(5):805.
[70]	VELAZQUEZ M A, HADELER K G, HERRMANN D, et al.In vivo oocyte IGF-1 priming increases inner cell mass proliferation of in vitro-formed bovine blastocysts[J].Theriogenology, 2012, 78(3):517-527.
[71]	CURRIN L, GLANZNER W G, GUTIERREZ K, et al.Optimizing swine in vitro embryo production with growth factor and antioxidant supplementation during oocyte maturation[J].Theriogenology, 2022, 194:133-143.
[72]	SHABANKAREH H K, ZANDI M.Developmental potential of sheep oocytes cultured in different maturation media:effects of epidermal growth factor, insulin-like growth factor I, and cysteamine[J].Fertil Steril, 2010, 94(1):335-340.
[73]	ORNITZ D M, ITOH N.The fibroblast growth factor signaling pathway[J].Wiley Interdiscip Rev Dev Biol, 2015, 4(3):215-266.
[74]	ALMEIDA A P, SARAIVA M V A, ALVES FILHO J G, et al.Gene expression and immunolocalization of fibroblast growth factor 2 in the ovary and its effect on the in vitro culture of caprine preantral ovarian follicles[J].Reprod Domest Anim, 2012, 47(1):20-25.
[75]	SANTOS J M, MENEZES V G, BARBERINO R S, et al.Immunohistochemical localization of fibroblast growth factor-2 in the sheep ovary and its effects on pre-antral follicle apoptosis and development in vitro[J].Reprod Domest Anim, 2014, 49(3):522-528.
[76]	LU C L, YAN J, ZHI X, et al.Basic fibroblast growth factor promotes macaque follicle development in vitro[J].Reproduction, 2015, 149(5):425-433.
[77]	BARROS R G, LIMA P F, SOARES A C S, et al.Fibroblast growth factor 2 regulates cumulus differentiation under the control of the oocyte[J].J Assist Reprod Genet, 2019, 36(5):905-913.
[78]	DU C, DAVIS J S, CHEN C, et al.FGF2/FGFR signaling promotes cumulus-oocyte complex maturation in vitro[J].Reproduction, 2021, 161(2):205-214.
[79]	MONDAL S, MOR A, REDDY I J, et al.Effect of fibroblast growth factor 2(FGF2) and insulin transferrin selenium (ITS) on in vitro maturation, fertilization and embryo development in sheep[J].Braz Arch Biol Technol, 2015, 58(4):521-525.
[80]	FIELDS S D, HANSEN P J, EALY A D.Fibroblast growth factor requirements for in vitro development of bovine embryos[J]. Theriogenology, 2011, 75(8):1466-1475.
[81]	LI S H, HWU Y M, LU C H, et al.VEGF and FGF2 improve revascularization, survival, and oocyte quality of cryopreserved, subcutaneously-transplanted mouse ovarian tissues[J].Int J Mol Sci, 2016, 17(8):1237.
[82]	FISCHER P, HILFIKER-KLEINER D.Role of gp130-mediated signalling pathways in the heart and its impact on potential therapeutic aspects[J].Br J Pharmacol, 2008, 153(S1):S414-S427.
[83]	TANG Y, LUO Y, JIANG Z L, et al.Jak/Stat3 signaling promotes somatic cell reprogramming by epigenetic regulation[J].Stem Cells, 2012, 30(12):2645-2656.
[84]	VENDRELL-FLOTATS M, GARCÍA-MARTÍNEZ T, MARTÍNEZ-RODERO I, et al.In vitro maturation in the presence of leukemia inhibitory factor modulates gene and miRNA expression in bovine oocytes and embryos[J].Sci Rep, 2020, 10(1):17777.
[85]	SPATE L D, MURPHY S L, BENNE J A, et al.In vitro-matured gilt oocytes can have equal or better developmental competence than sow oocytes with new maturation media[J].Reprod Fertil Dev, 2016, 29(1):150.
[86]	DE MATOS D G, MILLER K, SCOTT R, et al.Leukemia inhibitory factor induces cumulus expansion in immature human and mouse oocytes and improves mouse two-cell rate and delivery rates when it is present during mouse in vitro oocyte maturation[J]. Fertil Steril, 2008, 90(6):2367-2375.
[87]	DANG-NGUYEN T Q, HARAGUCHI S, KIKUCHI K, et al.Leukemia inhibitory factor promotes porcine oocyte maturation and is accompanied by activation of signal transducer and activator of transcription 3[J].Mol Reprod Dev, 2014, 81(3):230-239.
[88]	MO X H, WU G Q, YUAN D S, et al.Leukemia inhibitory factor enhances bovine oocyte maturation and early embryo development[J].Mol Reprod Dev, 2014, 81(7):608-618.
[89]	PTAK G, LOPES F, MATSUKAWA K, et al.Leukaemia inhibitory factor enhances sheep fertilization in vitro via an influence on the oocyte[J].Theriogenology, 2006, 65(9):1891-1899.
[90]	AN L Y, LIU J, DU Y Y, et al.Synergistic effect of cysteamine, leukemia inhibitory factor, and Y27632 on goat oocyte maturation and embryo development in vitro[J].Theriogenology, 2018, 108:56-62.
[91]	KOCYIGIT A, CEVIK M.Effects of leukemia inhibitory factor and insulin-like growth factor-I on the cell allocation and cryotolerance of bovine blastocysts[J].Cryobiology, 2015, 71(1):64-69.
[92]	SERRANO ALBAL M, SILVESTRI G, KIAZIM L G, et al.Supplementation of porcine in vitro maturation medium with FGF2, LIF, and IGF1 enhances cytoplasmic maturation in prepubertal gilts oocytes and improves embryo quality[J].Zygote, 2022, 30(6):801-808.
[93]	STOECKLEIN K S, ORTEGA M S, SPATE L D, et al.Improved cryopreservation of in vitro produced bovine embryos using FGF2, LIF, and IGF1[J].PLoS One, 2021, 16(2):e0243727.
[94]	TIAN H, QI Q, YAN F X, et al.Enhancing the developmental competence of prepubertal lamb oocytes by supplementing the in vitro maturation medium with sericin and the fibroblast growth factor 2-leukemia inhibitory factor-Insulin-like growth factor 1 combination[J].Theriogenology, 2021, 159:13-19.
[95]	SU Y Q, DENEGRE J M, WIGGLESWORTH K, et al.Oocyte-dependent activation of mitogen-activated protein kinase (ERK1/2) in cumulus cells is required for the maturation of the mouse oocyte-cumulus cell complex[J].Dev Biol, 2003, 263(1):126-138.
[96]	MEINECKE B, KRISCHEK C.MAPK/ERK kinase (MEK) signalling is required for resumption of meiosis in cultured cumulus-enclosed pig oocytes[J].Zygote, 2003, 11(1):7-16.
[97]	PROCHÁZKA R, PETLACH M, NAGYOVÁ E, et al.Effect of epidermal growth factor-like peptides on pig cumulus cell expansion, oocyte maturation, and acquisition of developmental competence in vitro:Comparison with gonadotropins[J]. Reproduction, 2011, 141(4):425-435.
[98]	TONG C, FAN H Y, CHEN D Y, et al.Effects of MEK inhibitor U0126 on meiotic progression in mouse oocytes:Microtuble organization, asymmetric division and metaphase II arrest[J].Cell Res, 2003, 13(5):375-383.
[99]	PROCHÁZKA R, BARTKOVÁ A, NĚMCOVÁ L, et al.The role of MAPK3/1 and AKT in the acquisition of high meiotic and developmental competence of porcine oocytes cultured in vitro in FLI medium[J].Int J Mol Sci, 2021, 22(20):11148.
[100]	STEFANELLO J R, BARRETA M H, PORCIUNCULA P M, et al.Effect of angiotensin II with follicle cells and insulin-like growth factor-I or insulin on bovine oocyte maturation and embryo development[J].Theriogenology, 2006, 66(9):2068-2076.

A Review of Optimization of in vitro Maturation System of OPU Oocytes

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 100

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	MIAO Shu, AN Jishan, WANG Zuo, XIAO Dingfu, LAN Xinyi, LIU Lei, SHEN Weijun, WAN Fachun. Leucine Promotes the Proliferation of Bovine Myoblasts through PI3K-AKT Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 142-152.
[2]	LI Huihui, DONG Keer, LIU Xinbo, ZHANG Chunxiao, MA Chao, CHEN Liping, ZHONG Qi, YAO Gang, MA Xuelian. Establishment and Application of Rapid Detection Method for Bovine Norovirus and Bovine Rotavirus Dual RAA-LFD [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 406-412.
[3]	WANG Dongliang, REN Jing, HAO Qinqin, LI Pengfei. Identification and Transcriptional Regulation Analysis of Core Promoter of Bovine CART Gene [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3689-3699.
[4]	SHEN Yingchao, DAVSHILT Toli, REN Hong, WANG Xisheng, TIAN Shuyue, DU Ming, DUGARJAVIIN Manglai, BOU Gerelchimeg. Differential Expression of Oocyte Development-related Hormone and Growth Factor Receptors in Equine Expanded and Compact Cumulus-oocyte Complexes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3735-3744.
[5]	XU Xi, YANG Baigao, ZHANG Hang, FENG Xiaoyi, HAO Haisheng, DU Weihua, ZHU Huabin, ZHANG Peipei, ZHAO Xueming. Effects of NMN on Lipid Droplet Content and Cryopreservation Effect of Bovine Oocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3348-3357.
[6]	WANG Wanjie, CHEN Nanzhu, ZOU Huiying, ZHOU Xinyi, HAO Haisheng, PANG Yunwei, ZHU Huabin, ZHAO Xueming, YU Dawei, DU Weihua. Effects of Histone Methyltransferase ASH1L Overexpression on Proliferation and Apoptosis of Bovine Cumulus Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3358-3368.
[7]	HUANG Jiang, LI Chuang, CUI Yueqi, YUAN Xueying, ZHAO Zhicheng, LIU Yu, ZHOU Yulong, ZHU Zhanbo, ZHANG Zecai. Study on the Effect of Gut Microbiota Disturbance on Susceptibility to BVDV Based on a Mouse Model [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3466-3473.
[8]	ZHANG Hang, YANG Baigao, XU Xi, FENG Xiaoyi, DU Weihua, HAO Haisheng, ZHU Huabin, ZHANG Peipei, ZHAO Xueming. Research Progress on the Mechanism of Heat Stress Affecting the Development of Dairy Cow Embryos [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2692-2700.
[9]	ZHANG Renbao, ZHOU Donghui, ZHOU Lisheng, GAO Xiaoxiao, LIU Nan, HE Jianning. Analysis of Genetic Structure of Conservation Population in Jining Gray Goats Based on 70 K SNP Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2836-2847.
[10]	YUAN Kaimin, SHI Yuxin, DONG Zhihao, ZHAO Lingjun, XU Shiyuan, WU Kaihui, WANG Dong. Research Progress on Early Pregnancy Diagnosis Technologies in Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2223-2230.
[11]	ZHAO Zhenjian, WANG Shujie, CHEN Dong, JI Xiang, SHEN Qi, YU Yang, CUI Shengdi, WANG Junge, CHEN Ziyang, TANG Guoqing. Population Structure and Genetic Diversity Analysis of Neijiang Pigs Based on Low-coverage Whole Genome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2297-2307.
[12]	MA Keyan, HAN Jintao, BAI Yaqin, LI Taotao, MA Youji. Genetic Diversity Analysis of Yongdeng Qishan Sheep Based on Specific-Locus Amplified Fragment Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1939-1950.
[13]	CHANG Yiming, TANG Cheng, YUE Hua. Molecular Epidemiological Investigation of Bovine Respiratory Syncytial Virus in Yaks [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2030-2041.
[14]	ZHU Jiaqiao, CHENG Laiyang, CAO Jiangqin, ZHU Min, LI Junwei, JU Huimin, LIU Zongping. Preliminary Study on the Location and Function of XRCC1 in Oocyte and Early Embryos [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2126-2133.
[15]	LI Yicong, PU Feiyang, FENG Xili, WANG Mengzhu, ZHAO Zeyang, ZHANG Derong, MA Zhongren, ZHOU Jianhua. Research Progress on Immunological Characteristics of Bovine Viral Diarrhea Virus Protein and Vaccines [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1381-1391.