OPU卵母细胞体外成熟体系的优化研究进展

doi:10.11843/j.issn.0366-6964.2023.04.002

Abstract

Abstract: Recent years, techniques for in vitro embryo production (IVP) have advanced significantly, especially the ovum pick-up (OPU) technology grows widely. OPU is a new technology developed in the 1990 s that can obtain oocytes from excellent breeding heifers, which has a broad application prospect in the research of the mechanism of animal embryo production and development and in the clinical practice of human assisted reproductive technology. It can improve the resource protection efficiency of breeding females and precious wild animals, and the genetic potential of excellent females, and provide the necessary supporting technology for the development of cattle biotechnology. The quality of oocytes is the key to the success of the IVP system, and the in vitro maturation (IVM) process of the oocytes is critical for successful fertilization and embryo development. Oocyte IVM completes the cytoplasmic and nuclear maturation of the oocyte, preparing all necessary components for development to the activation of the embryonic genome. Developmental ability of oocytes matured in vitro is lower than that of oocytes matured in vivo, non-synchronization of oocyte meiosis and nucleocytoplasmic maturation, oxidative stress damage, et al are important for successful fertilization and embryo development. Therefore, it is necessary to further improve the in vitro maturation ability of oocytes to improve the bovine IVP system. In this paper, the problems of in vitro maturation of oocytes such as non-synchronization of nucleocytoplasmic maturation and oxidative stress were summarized, as well as the improvement effects of C-type natriuretic peptide, melatonin, FLI and other substances on oocytes IVM were discussed in order to improve the bovine IVP system.

Key words: OPU, oocyte, in vitro maturation, bovine

CLC Number:

S814.6

ZHANG Peipei, HAO Haisheng, DU Weihua, ZHU Huabin, LI Shujing, YU Wenli, ZHAO Xueming. A Review of Optimization of in vitro Maturation System of OPU Oocytes[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1359-1369.

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A Review of Optimization of in vitro Maturation System of OPU Oocytes

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