雌性牦牛主要生殖器官中DBI的表达与定位分析

doi:10.11843/j.issn.0366-6964.2022.01.017

Abstract

Abstract: The study aimed to investigate the function of DBI gene and reveal its role in reproductive physiology of yak. The ovaries, fallopian tubes and uterine tissues of healthy female yaks (3-6 years old) at different stages of reproductive cycle (follicular stage, luteal stage and gestation stage) were collected and divided into 9 groups with 3 biological replicates in each group. Real-time quantitative PCR (qRT-PCR) and Western blotting (WB) assays were used to detect the rela-tive expression of DBI in reproductive organs of female yaks at different stages of reproductive cycle. Immunohistochemistry (IHC) was used to determine the distribution of DBI protein in the ovaries, fallopian tubes and uterus of female yaks. The results showed as follows: 1) qRT-PCR result showed that,in ovary,the expression of DBI was significantly higher in luteal stage and pregnancy stage than in follicular stage (P<0.05); In fallopian tube, the expression of DBI in luteal stage was significantly higher than that in follicular and gestation stages (P<0.05). In utero, the expression level of DBI was highest during gestation stage, followed by luteal phase, and the lowest during follicular phase. 2) Western blotting results showed that,in ovary, the level of DBI was significantly higher in pregnancy stage than in follicular and luteal stages (P<0.05); In fallopian tube, the level of DBI in luteal stage was significantly higher than that in follicular stage and gestation stage (P<0.05); In uterus, the expression level of DBI in pregnancy stage was significantly higher than that in luteal phase and follicular phase (P<0.05). 3) IHC results showed that DBI was mainly located in reproductive epithelial cells, granulosa cells, follicular membrane cells, luteal cells, tubal epithelial cells, uterine stromal cells and uterine glands. The expression levels of DBI in the ovaries, fallopian tubes and uterus tissues of yaks at different stages of reproductive cycle are significantly different, indicating that DBI is involved in the reproductive physiological regulation of yaks, which lays a theoretical foundation for further exploring the role of DBI gene in the reproductive aspects of plateau mammals.

Key words: yak, DBI, ovary, oviduct, uterus, expression

CLC Number:

S823.85

WANG Qianglong, PAN Yangyang, YAN Cuixia, ZHANG Tongxiang, HE Honghong, CUI Yan, XU Gengquan, WANG Libin, FAN Jiangfeng, YU Sijiu. Expression and Localization of DBI in Main Reproductive Organs of Female Yaks[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 169-178.

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Expression and Localization of DBI in Main Reproductive Organs of Female Yaks

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