白果内酯对IL-1β诱导的ATDC5软骨细胞自噬、增殖和凋亡的影响

doi:10.11843/j.issn.0366-6964.2023.02.039

Abstract

Abstract: The purpose of this study was to investigate the effect of bilobalide on IL-1β-induced autophagy, proliferation and apoptosis of ATDC5 chondrocytes. 10 ng·mL^-1 IL-1β was used to induce ATDC5 chondrocytes to construct an in vitro inflammatory model, and then the cells were randomly divided into control group, IL-1β group and bilobalide groups (low-, medium- and high-dosage). The newly synthesized DNA of cells was detected by EdU, and the cell proliferation rate was detected by double labeling combined with the nuclear marker (Hoechest). The apoptosis of ATDC5 chondrocytes was analyzed by flow cytometry using membrane Annexin-V/PI. Autophagic flux was detected by a combined measurement of the mRFP-GFP-LC3 dual fluorescence system. Western blot and qRT-PCR methods were used to detect the protein and mRNA expression levels of LC3-II, Beclin1, BAX, Caspase-3 and Bcl2 in chondrocytes of each group. The results showed that after IL-1β-induced ATDC5 chondrocytes, cell proliferation was attenuated, autophagy was down-regulated and apoptosis was improved. Bilobalide intervention can promote autophagy, proliferation and inhibit apoptosis. Bilobalide promoted the proliferation of IL-1β-induced ATDC5 chondrocytes (P<0.05). Compared with IL-1β group, bilobalide inhibited the protein and mRNA expression of LC3-II, Beclin1, BAX and Caspase-3 (P<0.05), and promoted the protein and mRNA expression of Bcl2 (P<0.05). Autophagy flux was activated, and the apoptosis rate was significantly reduced (P<0.05). The results suggest that bilobalide can promote autophagy of IL-1β-induced ATDC5 chondrocytes and inhibit cell apoptosis, promote cell proliferation and protect chondrocytes.

Key words: articular cartilage, bilobalide, autophagy, apoptosis, cell proliferation, chondrocytes

CLC Number:

S853.74

MA Tianwen, YU Yue, LÜ Liangyu, JIA Lina, RUAN Hongri, WANG Haoran, WANG Xinyu, ZHANG Yuxin, ZHANG Jiantao, GAO Li. Effects of Bilobalide on Autophagy, Proliferation and Apoptosis of IL-1β-induced ATDC5 Chondrocytes[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 837-846.

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Effects of Bilobalide on Autophagy, Proliferation and Apoptosis of IL-1β-induced ATDC5 Chondrocytes

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