仔鼠哺乳期补充参苓白术散改善抗生素诱导的肠道稳态失衡

doi:10.11843/j.issn.0366-6964.2023.02.038

Abstract

Abstract: This study aimed to investigate the mechanism of Shenling Baizhu powder on improving intestinal homeostasis and relieving weaning stress of newborn rats. Nine pregnant SD rats were randomly divided into three groups, including blank group (CON), antibiotics group (ABX) and Shenling Baizhu powder group (SLBZS). After delivery, antibiotics were added to the drinking water of the female rats in ABX group and SLBZS group, the female rats in CON group were treated normally, and the offsprings in SLBZS group were gavaged with Shenling Baizhu powder decoction for 7 consecutive days from the 14th day after delivery, the offsprings in CON group and ABX group were gavaged with the same amount of normal saline. On day 14 and day 21 after delivery, the fecal samples of the offsprings in each group were collected and analyzed by 16S rRNA gene sequencing. Western blot was used to detect the expression of ZO-1 and Occludin proteins in the colon of the offsprings. Moreover, the histopathological changes of the colon section were detected by hematoxylin and eosin (HE) staining. The results showed that the intestinal microbial diversity in ABX group was significantly lower than that in CON group (P<0.05), and the Shenling Baizhu powder treatment reduced the relative abundance of pathogenic bacteria, such as Enterococcus, Sutterella, and Shigella, while increased the relative abundance of Lactobacillus, Blautia and Akkermansia. The expression of tight junction proteins ZO-1 and Occludin in colon of offspring rats were significantly lower than CON group (P<0.05). After treated with Shenling Baizhu powder, the gut barrier of offspring rats had been improved. The histopathological section of the colon showed obvious inflammatory cell infiltration in the ABX group, while the inflammatory cell infiltration was reduced in the SLBZS group. In conclusion, the use of antibiotics in maternal rats during the period of lactation could change the gut microbes structure in offspring rats, which may affect the permeability of the intestinal mucosa, and has the potential to promote intestinal inflammation. The use of Shenling Baizhu powder on sucking rats could modulate the structure of gut microbiota and increase the expression level of intestinal tight junction protein ZO-1 and Occludin, thus improving the health of intestinal.

Key words: Shenling Baizhu powder, intestinal microbiota, mucosal barrier, weaning stress

CLC Number:

S853.74

ZHANG Wenchang, WANG Zhihua, LIAN Jiale, QU Qian, LÜ Weijie, CHEN Shu'ai, GUO Shining. Supplement of Shenling Baizhu Powder to Offspring Rats during Sucking Improved Intestinal Dyshomeostasis Induced by Antibiotics[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 825-836.

References 48

[1]	DOMINGUEZ-BELLO M G, COSTELLO E K, CONTRERAS M, et al.Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns[J].Proc Natl Acad Sci U S A, 2010, 107(26):11971-11975.
[2]	NURIEL-OHAYON M, NEUMAN H, KOREN O.Microbial changes during pregnancy, birth, and infancy[J].Front Microbiol, 2016, 7:1031.
[3]	NURIEL-OHAYON M, NEUMAN H, ZIV O, et al.Progesterone increases Bifidobacterium relative abundance during late pregnancy[J].Cell Rep, 2019, 27(3):730-736.e3.
[4]	KOENIG J E, SPOR A, SCALFONE N, et al.Succession of microbial consortia in the developing infant gut microbiome[J].Proc Natl Acad Sci U S A, 2011, 108(S1):4578-4585.
[5]	KORPELA K, SALONEN A, VIRTA L J, et al.Intestinal microbiome is related to lifetime antibiotic use in Finnish pre-school children[J].Nat Commun, 2016, 7:10410.
[6]	TANAKA S, KOBAYASHI T, SONGJINDA P, et al.Influence of antibiotic exposure in the early postnatal period on the development of intestinal microbiota[J].FEMS Immunol Med Microbiol, 2009, 56(1):80-87.
[7]	ANTONOPOULOS D A, HUSE S M, MORRISON H G, et al.Reproducible community dynamics of the gastrointestinal microbiota following antibiotic perturbation[J].Infect Immun, 2009, 77(6):2367-2375.
[8]	杨茜雅, 谢磊, 赵永攀, 等.犊牛断奶应激的原因、影响和评估[J].中国乳业, 2021(10):31-34.YANG X Y, XIE L, ZHAO Y P, et al.Causes, effects and evaluation of weaning stress in calves[J].China Dairy, 2021(10):31-34.(in Chinese)
[9]	万凡, 钟儒清, 刘明, 等.断奶仔猪应激期肠道健康及营养调控的研究进展[J].中国畜牧杂志, 2021, 57(3):25-30.WAN F, ZHONG R Q, LIU M, et al.Research progress on intestinal health and nutritional regulation of weaned piglets during stress[J].Chinese Journal of Animal Science, 2021, 57(3):25-30.(in Chinese)
[10]	SUZUKI T.Regulation of the intestinal barrier by nutrients:the role of tight junctions[J].Anim Sci J, 2020, 91(1):e13357.
[11]	SOMMER F, ANDERSON J M, BHARTI R, et al.The resilience of the intestinal microbiota influences health and disease[J].Nat Rev Microbiol, 2017, 15(10):630-638.
[12]	SÁNCHEZ B, URDACI M C.Extracellular proteins from Lactobacillus plantarum BMCM12 prevent adhesion of enteropathogens to mucin[J].Curr Microbiol, 2012, 64(6):592-596.
[13]	YIN M M, YAN X B, WENG W H, et al.Micro integral membrane protein (MIMP), a newly discovered anti-inflammatory protein of Lactobacillus plantarum, enhances the gut barrier and modulates microbiota and inflammatory cytokines[J].Cell Physiol Biochem, 2018, 45(2):474-490.
[14]	EVERARD A, BELZER C, GEURTS L, et al.Cross-talk between Akkermansia muciniphila and intestinal epithelium controls diet-induced obesity[J].Proc Natl Acad Sci U S A, 2013, 110(22):9066-9071.
[15]	张萌, 张向东, 夏永欣, 等.参苓白术散合附子理中丸联合西药治疗克罗恩病临床研究[J].新中医, 2019, 51(12):73-76.ZHANG M, ZHANG X D, XIA Y X, et al.Clinical study on Shenling Baizhu powder combined with Fuzi Lizhong pills and western medicine in the treatment of Crohn's disease[J].Journal of New Chinese Medicine, 2019, 51(12):73-76.(in Chinese)
[16]	姜宝山.桃花汤合参苓白术散加减治疗溃疡性结肠炎的疗效观察[J].中国医药指南, 2019, 17(33):223.JIANG B S.Therapeutic effect of Taohua decoction combined with Shenling Baizhu powder on ulcerative colitis[J].Guide of China Medicine, 2019, 17(33):223.(in Chinese)
[17]	崔爱珍.参苓白术散联合理中汤对慢性功能性腹泻患者的临床效果[J].世界最新医学信息文摘, 2019, 19(91):182-183.CUI A Z.Clinical effect of Shenling Baizhu powder combined with Li Zhong Decoction on patients with chronic functional diarrhea[J].World Latest Medicine Information, 2019, 19(91):182-183.(in Chinese)
[18]	AL NABHANI Z, DULAUROY S, MARQUES R, et al.A weaning reaction to microbiota is required for resistance to immunopathologies in the adult[J].Immunity, 2019, 50(5):1276-1288.e5.
[19]	NYANGAHU D D, LENNARD K S, BROWN B P, et al.Disruption of maternal gut microbiota during gestation alters offspring microbiota and immunity[J].Microbiome, 2018, 6(1):124.
[20]	GONZALEZ-PEREZ G, HICKS A L, TEKIELI T M, et al.Maternal antibiotic treatment impacts development of the neonatal intestinal microbiome and antiviral immunity[J].J Immunol, 2016, 196(9):3768-3779.
[21]	雷英, 贺志有, 刘丽莎, 等.参苓白术散对脾虚证小鼠血清淀粉酶、D-木糖、胃泌素及小肠组织学变化的研究[J].中药药理与临床, 2012, 28(2):6-9.LEI Y, HE Z Y, LIU L S, et al.Shenlingbaizhu powde of spleen deficiency inserum amylase, D-xylose, gastrin and intestinal histological changes in experimental study[J].Pharmacology and Clinics of Chinese Materia Medica, 2012, 28(2):6-9.(in Chinese)
[22]	张志谦, 赵斌, 肖秋平, 等.基于miR-21、miR-150和miR-200b研究参苓白术散对克罗恩病大鼠肠黏膜屏障的干预机制[J].中医临床研究, 2021, 13(25):8-13.ZHANG Z Q, ZHAO B, XIAO Q P, et al.The intervention mechanism of Shenling Baizhu San on miR-21, miR-150 and miR-200b in intestinal mucosal barrier of rats with Crohn's disease[J].Clinical Journal of Chinese Medicine, 2021, 13(25):8-13.(in Chinese)
[23]	张超, 吕伟杰, 雷丰瑞, 等.参苓白术散对哺乳仔猪生长性能及肠道菌群的影响[J].中国兽医学报, 2017, 37(6):1155-1160.ZHANG C, LYU W J, LEI F R, et al.Effect of Shenling Baizhu san on growth performance and structural changes of gut microbiota in nursing piglets[J].Chinese Journal of Veterinary Science, 2017, 37(6):1155-1160.(in Chinese)
[24]	WANG K, LIAO M F, ZHOU N, et al.Parabacteroides distasonis alleviates obesity and metabolic dysfunctions via production of succinate and secondary bile acids[J].Cell Rep, 2019, 26(1):222-235.e5.
[25]	WANG L, ZENG B H, LIU Z W, et al.Green tea polyphenols modulate colonic microbiota diversity and lipid metabolism in high-fat diet treated HFA mice[J].J Food Sci, 2018, 83(3):864-873.
[26]	CHANG C J, LIN C S, LU C C, et al.Correction:Corrigendum:Ganoderma lucidum reduces obesity in mice by modulating the composition of the gut microbiota[J].Nat Commun, 2017, 8:16130.
[27]	PHAM T P T, ALOU M T, BACHAR D, et al.Gut microbiota alteration is characterized by a proteobacteria and fusobacteria bloom in kwashiorkor and a bacteroidetes paucity in marasmus[J].Sci Rep, 2019, 9(1):9084.
[28]	SHIN N R, WHON T W, BAE J W.Proteobacteria:Microbial signature of dysbiosis in gut microbiota[J].Trends Biotechnol, 2015, 33(9):496-503.
[29]	汪富文, 罗小雨, 王朝飞, 等.动物脾脏发育研究进展[J].家畜生态学报, 2021, 42(12):9-13.WANG F W, LUO X Y, WANG Z F, et al.Research progress on the development of animal spleen[J].Journal of Domestic Animal Ecology, 2021, 42(12):9-13.(in Chinese)
[30]	田艳, 刘青青, 田海霞, 等.益生菌缓解环磷酰胺所致小鼠免疫功能低下[J].中国免疫学杂志, 2020, 36(15):1805-1808.TIAN Y, LIU Q Q, TIAN H X, et al.Probiotics alleviate immunosuppression induced by cyclophosphamide in mice[J].Chinese Journal of Immunology, 2020, 36(15):1805-1808.(in Chinese)
[31]	IANIRO G, TILG H, GASBARRINI A.Antibiotics as deep modulators of gut microbiota:Between good and evil[J].Gut, 2016, 65(11):1906-1915.
[32]	AZAD M B, KONYA T, PERSAUD R R, et al.Impact of maternal intrapartum antibiotics, method of birth and breastfeeding on gut microbiota during the first year of life:A prospective cohort study[J].BJOG, 2016, 123(6):983-993.
[33]	LV W J, LIU C, YE C X, et al.Structural modulation of gut microbiota during alleviation of antibiotic-associated diarrhea with herbal formula[J].Int J Biol Macromol, 2017, 105(Pt 3):1622-1629.
[34]	WANG S, MARTINS R, SULLIVAN M C, et al.Diet-induced remission in chronic enteropathy is associated with altered microbial community structure and synthesis of secondary bile acids[J].Microbiome, 2019, 7(1):126.
[35]	FERRARI M L, MALARDÉ V, GRASSART A, et al.Shigella promotes major alteration of gut epithelial physiology and tissue invasion by shutting off host intracellular transport[J].Proc Natl Acad Sci U S A, 2019, 116(27):13582-13591.
[36]	ABT M C, MCKENNEY P T, PAMER E G.Clostridium difficile colitis:Pathogenesis and host defence[J].Nat Rev Microbiol, 2016, 14(10):609-620.
[37]	MICHAEL G B, BOSSÉ J T, SCHWARZ S.Antimicrobial resistance in Pasteurellaceae of veterinary origin[J].Microbiol Spectr, 2018, 6(3):10.
[38]	HU Y F, YANG X, QIN J J, et al.Metagenome-wide analysis of antibiotic resistance genes in a large cohort of human gut microbiota[J].Nat Commun, 2013, 4:2151.
[39]	ZHOU K Q.Strategies to promote abundance of Akkermansia muciniphila, an emerging probiotics in the gut, evidence from dietary intervention studies[J].J Funct Foods, 2017, 33:194-201.
[40]	WANG L J, TANG L, FENG Y M, et al.A purified membrane protein from Akkermansia muciniphila or the pasteurised bacterium blunts colitis associated tumourigenesis by modulation of CD8+ T cells in mice[J].Gut, 2020, 69(11):1988-1997.
[41]	ZHOU D, PAN Q, XIN F Z, et al.Sodium butyrate attenuates high-fat diet-induced steatohepatitis in mice by improving gut microbiota and gastrointestinal barrier[J].World J Gastroenterol, 2017, 23(1):60-75.
[42]	MUKHERJEE A, LORDAN C, ROSS R P, et al.Gut microbes from the phylogenetically diverse genus Eubacterium and their various contributions to gut health[J].Gut Microbes, 2020, 12(1):1802866.
[43]	LIN Y L, LI B J, YANG X G, et al.Non-hematopoietic STAT6 induces epithelial tight junction dysfunction and promotes intestinal inflammation and tumorigenesis[J].Mucosal Immunol, 2019, 12(6):1304-1315.
[44]	HAYES C L, DONG J, GALIPEAU H J, et al.Commensal microbiota induces colonic barrier structure and functions that contribute to homeostasis[J].Sci Rep, 2018, 8(1):14184.
[45]	SAITOH Y, SUZUKI H, TANI K, et al.Structural insight into tight junction disassembly by Clostridium perfringens enterotoxin[J].Science, 2015, 347(6223):775-778.
[46]	CHELAKKOT C, CHOI Y, KIM D K, et al.Akkermansia muciniphila-derived extracellular vesicles influence gut permeability through the regulation of tight junctions[J].Exp Mol Med, 2018, 50(2):e450.
[47]	LIU L X, LI Q Q, YANG Y J, et al.Biological function of short-chain fatty acids and its regulation on intestinal health of poultry[J].Front Vet Sci, 2021, 8:736739.
[48]	HUANG X Y, OSHIMA T, TOMITA T, et al.Butyrate alleviates cytokine-induced barrier dysfunction by modifying claudin-2 levels[J].Biology (Basel), 2021, 10(3):205.

Supplement of Shenling Baizhu Powder to Offspring Rats during Sucking Improved Intestinal Dyshomeostasis Induced by Antibiotics

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