高脂高糖饮食对小型猪肠道微生物的影响

doi:10.11843/j.issn.0366-6964.2022.04.014

Abstract

Abstract: High-energy diet is one of the important causes of obesity, and intestinal microorganisms play an important role in it. The aim of this study is to explore the dynamic changes of intestinal microbial composition in the process of high-fat and high-sugar diet and screen the intestinal microorganisms which closely related to the development of obesity. In this study, Guangxi Bama mini-pigs were randomly divided into normal diet group (CN group) and high-fat and high-sugar diet group (HFD group) for 30 d of dietary intervention. Fecal samples were collected at 0, 7, 15 and 30 d after intervention, respectively. The effects of high-fat and high-sugar diet on intestinal microbial composition, structure and function of Guangxi Bama mini-pigs were analyzed by 16S rRNA gene sequencing. The results showed that the diversity of intestinal microbiota in HFD group was generally lower than that of CN group, and high-fat and high-sugar diet changed the structure of intestinal microflora in mini-pigs. With the increase of intervention time of high-fat and high-sugar diet, the overall change trend of intestinal microbiota was observed. At the phylum level, the abundance of Firmicutes increased while Bacteroidetes decreased gradually in the pigs of HFD group. At the genus level, the abundance of Lactobacillus decreased gradually while the abundance of Ruminococcus increased continuously in the HFD group. Twelve bacterial genuses, including g__Lactobacillus, g__norank_f__p_251_o5 and g__unclassified_c__Bacilli, were significantly enriched in the CN group, while 8 genuses including g__Peptococcus, g__Collinsella and g__Senegalimassilia were significantly enriched in the HFD group, which might be potential microorganisms related to obesity. Microbial function prediction analysis found no significant difference in microbial function between the two groups, which were mainly enriched in amino acid biosynthesis, starch and sucrose metabolism, amino sugar and nucleotide sugar metabolism, glycolysis and gluconeogenesis. This study indicated that the high-fat and high-sugar diet reduced the diversity of intestinal microorganisms, changed the microbial structure, resulting in a certain imbalance of gut microbiota, and some potential microorganisms which were closely related to development of obesity were found.

Key words: mini-pig, obesity, high-fat and high-sugar diet, intestinal microbiota

CLC Number:

TIAN Weilong, SI Jinglei, LIU Xiaoxiao, QI Wenjing, CHEN Kuirong, CHENG Feng, LI Yueyue, Lü Dongling, LIANG Liang, GAO Jiuyu, FENG Lingli, MO Jiayuan, LAN Ganqiu, LIANG Jing. Effects of High-fat and High-sugar Diet on Intestinal Microbiota in Mini-pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1143-1153.

References 35

[1]	NG M, FLEMING T, ROBINSON M, et al. Global, regional, and national prevalence of overweight and obesity in children and adults during 1980-2013: a systematic analysis for the Global Burden of Disease Study 2013 [J]. Lancet, 2014, 384(9945): 766-781.
[2]	WHO. Obesity and overweight [EB/OL]. (2020-04-01) [2021-05-20].https://www.who.int/zh/news-room/fact-sheets/detail/obesity-and-overweight.
[3]	FLEGAL K M, GRAUBARD B I, WILLIAMSON D F, et al. Cause-Specific Excess Deaths Associated With Underweight, Overweight, and Obesity [J]. JAMA, 2007, 298(17): 2028-2037.
[4]	HOTAMISLIGIL G S. Inflammation and metabolic disorders [J]. Nature, 2006, 444(7121): 860-867.
[5]	NAHUM M S, VANIA C R, OSCAR R P, et al. New Aspects of Lipotoxicity in Nonalcoholic Steatohepatitis [J]. Int J Mol Sci, 2018, 19(7): 2034.
[6]	MCPHEE J B, SCHERTZER J D. Immunometabolism of obesity and diabetes: microbiota link compartmentalized immunity in the gut to metabolic tissue inflammation [J]. Clin Sci (Lond), 2015, 129(12): 1083-1096.
[7]	HILL J O. Understanding and addressing the epidemic of obesity: an energy balance perspective [J]. Endocr Rev, 2006, 27(7): 750-761.
[8]	ROSENBAUM M, KNIGHT R, LEIBEL R. The gut microbiota in human energy homeostasis and obesity [J]. Trends Endocrinol Metab, 2015, 26(9): 493-501.
[9]	LEY R, HAMADY M, LOZUPONE C, et al. Evolution of mammals and their gut microbes [J]. Science, 2008, 320(5883): 1647-1651.
[10]	O’HARA A M, SHANAHAN F. The gut flora as a forgotten organ [J]. EMBO Rep, 2006, 7(7): 688-693.
[11]	WOSTMANN B S, LARKIN C, MORIARTY A, et al. Dietary intake, energy metabolism, and excretory losses of adult male germfree Wistar rats [J]. Lab Anim Sci, 1983, 33(1): 46-50.
[12]	LEY R E, BÄCKHED F, TURNBAUGH P, et al. Obesity alters gut microbial ecology [J]. Proc Natl Acad Sci U S A, 2005, 102(31): 11070-11075.
[13]	CHEN S F, ZHOU Y Q, CHEN Y R, et al. fastp: an ultra-fast all-in-one FASTQ preprocessor [J]. Bioinformatics, 2018, 34(17): i884-i890.
[14]	MAGOČ T, SALZBERG S L. FLASH: fast length adjustment of short reads to improve genome assemblies [J]. Bioinformatics, 2011, 27(21): 2957-2963.
[15]	CALLAHAN B J, MCMURDIE P J, ROSEN M J, et al. DADA2: High-resolution sample inference from Illumina amplicon data [J]. Nat Methods, 2016, 13(7): 581-583.
[16]	王爱德, 兰干球, 郭亚芬. 广西巴马小型猪的培育 [J]. 实验动物科学, 2010, 27(1): 60-63.WANG A D, LAN G Q, GUO Y F. Genetic Breeding of Guangxi Bama mini-pig [J]. Laboratory Animal Science, 2010, 27(1): 60-63. (in Chinese)
[17]	张笠. 广西巴马小型猪全基因组测序及分析研究 [D]. 南宁：广西大学, 2019.ZHANG L. Genome sequencing and research of Bama mini-pig [D]. Nanning: Guangxi University, 2019. (in Chinese)
[18]	DONALDSON G, LEE S, MAZMANIAN S. Gut biogeography of the bacterial microbiota [J]. Nat Rev Microbiol, 2016, 14(1): 20-32.
[19]	相磊, 陈华, 赵德明. 高糖高脂饲料诱导的小型猪肥胖模型[J]. 实验动物科学, 2019, 36(4): 1-5,9.XIANG L, CHEN H, ZHAO D M, High fat and high sugar diet induced the fat model of Bama miniature pigs [J]. Laboratory Animal Science, 2019, 36(4): 1-5,9. (in Chinese)
[20]	HOOPER L V, WONG M H, THELIN A, et al. Molecular analysis of commensal host-microbial relationships in the intestine [J]. Science, 2001, 291(5505): 881-884.
[21]	LEY R E, TURNBAUGH P J, KLEIN S, et al. Microbial ecology: human gut microbes associated with obesity [J]. Nature, 2006, 444(7122): 1022-1023.
[22]	JUMPERTZ R, LE D S, TURNBAUGH P J, et al. Energy-balance studies reveal associations between gut microbes, caloric load, and nutrient absorption in humans [J]. Am J Clin Nutr, 2011, 94(1): 58-65.
[23]	DUNCAN S H, LOBLEY G E, HOLTROP G, et al. Human colonic microbiota associated with diet, obesity and weight loss [J]. Int J Obes (Lond), 2008, 32(11): 1720-1724.
[24]	YOO S R, KIM Y J, PARK D Y, et al. Probiotics L. plantarum and L. curvatus in combination alter hepatic lipid metabolism and suppress diet-induced obesity [J]. Obesity (Silver Spring, Md), 2013, 21(12): 2571-2578.
[25]	WANG J J, TANG H, ZHANG C H, et al. Modulation of gut microbiota during probiotic-mediated attenuation of metabolic syndrome in high fat diet-fed mice [J]. ISME J, 2015, 9(1): 1-15.
[26]	SIJPESTEIJN A K. Cellulose-decomposing bacteria from the rumen of cattle [J]. Antonie van Leeuwenhoek, 1949, 15(1): 49-52.
[27]	QIN J J, LI R Q, RAES J, et al. A human gut microbial gene catalogue established by metagenomic sequencing [J]. Nature, 2010,464(7285):59-65.
[28]	FLINT H J, BAYER E A, RINCON M T, et al. Polysaccharide utilization by gut bacteria: potential for new insights from genomic analysis [J]. Nat Rev Microbiol, 2008, 6(2): 121-131.
[29]	MORRISON D J, PRESTON T. Formation of short chain fatty acids by the gut microbiota and their impact on human metabolism [J]. Gut Microbes, 2016, 7(3): 189-200.
[30]	DEN BESTEN G, BLEEKER A, GERDING A, et al. Short-chain fatty acids protect against high-fat diet-induced obesity via a PPARγ-Dependent Switch From Lipogenesis to Fat Oxidation [J]. Diabetes, 2015, 64(7): 2398-2408.
[31]	TREMAROLI V, BÄCKHED F. Functional interactions between the gut microbiota and host metabolism [J]. Nature, 2012, 489(7415): 242-249.
[32]	CANDELA M, BIAGI E, SOVERNI M, et al. Modulation of gut microbiota dysbioses in type 2 diabetic patients by macrobiotic Ma-Pi 2 diet[J]. Br J Nutr, 2016, 116(1): 80-93.
[33]	LIU W J, ZHANG J C, WU C Y, et al. Unique Features of Ethnic Mongolian Gut Microbiome revealed by metagenomic analysis[J]. Sci Rep, 2016, 6(1): 34826.
[34]	MACH N, BERRI M, ESTELL É J, et al. Early-life establishment of the swine gut microbiome and impact on host phenotypes[J]. Environ Microbiol Rep, 2015, 7(3): 554-569.
[35]	KIROS T G, DERAKHSHANI H, PINLOCHE E, et al. Effect of live yeast Saccharomyces cerevisiae (Actisaf Sc 47) supplementation on the performance and hindgut microbiota composition of weanling pigs[J]. Sci Rep, 2018, 8(1): 5315.

Effects of High-fat and High-sugar Diet on Intestinal Microbiota in Mini-pigs

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 35

Related Articles 14

Recommended Articles

Metrics

Comments

[1]	LU Jiang, ZHU Daoxian, LU Jinye, LIU Li, HAO Fuxing, WU Zhi, LU Wei, LIU Jing. Inulin with High Degree of Polymerization Improves High-fat Diet Induced Obesity in Dogs by Regulating the Gut-adipose Tissue Axis [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3941-3950.
[2]	ZHENG Xianrui, ZHUO Mingxue, JI Jinli, JIANG Weihu, DENG Zaishuang, ZHANG Jicheng, TIAN Yali, DING Yueyun, ZHANG Xiaodong, YIN Zongjun. Characteristics of Serum Immune Indices and Intestinal Microbiota of Wannan Black Pigs at Different Growth Stages [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3770-3783.
[3]	JIAO Guangming, LÜ Yingguang, SANG Jinfang, KOU Zhipeng, LIU Tao, WANG Yue, LU Xiangyu, PIAO Chenxi, MA Yajun, ZHANG Jiantao, WANG Hongbin. Effect of Adipose Mesenchymal Stem Cells in Combination with Methylprednisolone on Allogeneic Skin Grafts in Minipigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3533-3545.
[4]	ZHAO Wanli, CAO Qiqi, YANG Yue, DENG Zhaoju, XU Chuang. The Interaction between Gastrointestinal Microbiota and Mucosal Immunity in Health of Perinatal Dairy Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2751-2760.
[5]	ZHU Qian, CHENG Yating, LI Ruixuan, LI Chenjian, LIU Yating, KONG Xiangfeng. Effects of Probiotics and Synbiotics Addition to Sows’ Diet on Fatty Acid Composition and Related Gene Expression in Muscle of Offspring Bama Mini-Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2458-2467.
[6]	GENG Meimei, DOU Mengying, FU Dezhi, HE Qinghua, KONG Xiangfeng. Effects of Intrauterine Growth Retardation on Developmental Model of Insulin-Like Growth Factor in Suckling Piglets of Huanjiang Mini-Pig [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2414-2420.
[7]	ZHANG Wenchang, WANG Zhihua, LIAN Jiale, QU Qian, LÜ Weijie, CHEN Shu'ai, GUO Shining. Supplement of Shenling Baizhu Powder to Offspring Rats during Sucking Improved Intestinal Dyshomeostasis Induced by Antibiotics [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 825-836.
[8]	WANG Yue, NAN Xuemei, JIANG Linshu, XIONG Benhai. Research Progress on the Correlation between Gastrointestinal Microbiota and Bovine Mastitis in Dairy Cows and Its Regulatory Potential for Mastitis [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2083-2092.
[9]	WANG Pei, WANG Lina, HUO Hailong, ZHANG Xia, ZHAO Xiao, WANG Xuefei, HUO Jinlong. cDNA Full-length Cloning, Tissue Expression and Subcellular Location of DAZL Gene in Pig [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 683-692.
[10]	HOU Meng, LIU Yigang, YE Mengjun, MAIMAITIYIMING·Maimaitili, MUNIRE·Tusong, WANG Jia'nan, KAIMAIREYA·Aihemaiti, TUREGULI·Alimu, WANG Jinquan. Study on the Difference of Intestinal Microbiota of Altay Sheep with Different Growth Rates [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(9): 2177-2186.
[11]	LIN Sen, LIN Ya-qiu, ZHU Jiang-jiang, XU Ya-ou, WANG Yong. Temporal-Spatial Characteristics of FTO Gene Expression in Tibetan Chicken and Its Correlation with Intramuscular Fat Content [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(7): 1191-1201.
[12]	YUE Min，TIAN Yu-guang，WAN Bin，PANG Wei，WU Qing-hong，WANG Yu-jue. The Impact of GHR Mutation on Suppressing the Growth in Tibet Mini-pig [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 882-887.
[13]	WANG Shu-yan, HUO Jin-long, WANG Pei, PAN Wei-rong, ZENG Yang-zhi. Cloning and Prokaryotic Expression of Growth Hormone Gene in Banna Mini-pig Inbred Line [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(10): 1669-1676.
[14]	The Molecular Genetic Study of Cytochrome b (Cyt b) Gene of Tibet Mini-pigs. The Molecular Genetic Study of Cytochrome b (Cyt b) Gene of Tibet Mini-pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(6): 769-773.