CYP19A1调控内源性雌激素合成促进牦牛COCs细胞自噬和早期发育能力

doi:10.11843/j.issn.0366-6964.2022.12.014

Abstract

Abstract: The aim of this study was to investigate the effects of cytochrome P450arom (CYP19A1) on the secretion of endogenous 17β-estradiol (E₂), and its effects on autophagy of oocyte and subsequent embryonic development were also evaluated. During the in vitro culture of the yak cumulus-oocyte complexes (COCs), saline, E₂ (10^-7 mol·L^-1), aflatoxin B1 (AFB1) and bisphenol A (BPA) were respectively supplemented to M199 at same volume. The expression and location of CYP19A1 in COCs from different groups were detected by qRT-PCR, Western blot and immunofluorescence, as well as the autophagy related genes 5(ATG5), Bcl-2 homologous domain protein (BECLIN1) and microtubule associated protein light chain 3 (LC3). The levels of endogenous E₂ in mature medium of COCs from the groups treated with saline, AFB1 and BPA were measured by enzyme-linked immunosorbent assay (ELISA). Rates of mature oocyte, cleavage embryo and blastocyst were calculated and compared among different groups. The results showed that both gene and protein levels of CYP19A1 were enhanced significantly in the AFB1 group, along with the increase of the endogenous secretion of E₂, ATG, BECLIN1 and LC3 levels in mature COCs (P<0.05). The level of CYP19A1 were reduced significantly in the E₂ group, while the expression of autophagy related factors in mature COCs were enhanced (P<0.05). In BPA treatment group, the CYP19A1 and endogenous E₂ were significantly reduced (P<0.05), which leading to the lower expressions of ATG5, BECLIN1 and LC3 (P<0.05). Comparing to the control group, the rates of mature oocyte and cleavage embryo were enhanced by E₂ and AFB1 (P<0.05), but reduced in BPA group (P<0.05). There was no significant difference about blastocyst rates among 4 groups (P>0.05). It can be concluded that endogenous E₂ was positive regulated by CYP19A1 during COCs maturation, furthermore both endogenous and exogenous E₂ can induce autophagy and improved the early developmental capacities of yak oocyte. The results provide a theoretical basis for further exploring the molecular mechanism of reproductive hormones regulating the maturation of oocytes in mammalian.

Key words: E₂, autophagy, cytochrome P450, oocyte maturation, embryo development

CLC Number:

S823.85

WANG Libin, WANG Meng, SUN Ying, CHEN Rui, ZHANG Tiantian, HUANG Zhenhua, ZHANG Qian, YU Sijiu, PAN Yangyang. CYP19A1 Promotes Autophagy and Early Developmental Ability of Yak Oocytes by Regulating the Levels of Endogenous Estradiol[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4283-4295.

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CYP19A1 Promotes Autophagy and Early Developmental Ability of Yak Oocytes by Regulating the Levels of Endogenous Estradiol

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