转录因子Foxq1通过WNT/β-catenin信号通路影响绒山羊毛囊干细胞增殖的研究

doi:10.11843/j.issn.0366-6964.2023.06.041

Abstract

Abstract: This study aimed to explore the function of Foxq1, a differentially expressed gene in the skin tissues at hair follicle induction stage (E 66) and cytodifferentiation stage (E 120), in hair follicle stem cells (HFSCs) of Shanbei white cashmere goat. In this study, 6 healthy pregnant Shanbei white cashmere goats with the same age and body weight were selected. And the back skin tissues were collected at E 66 (n=3) and E 120 (n=3) from the fetuses, respectively. The pAd-Foxq1 and pAd-Blank overexpression adenovirus vectors were transfected into HFSCs. EdU, MTT and flow cytometry were used to detect the effects of Foxq1 on the proliferation of HFSCs. qPCR and immunofluorescence assay were used to detect the expression of CTNNB1, a marker of activating Wnt signaling pathway, and the downstream genes of Wnt signaling pathway. The results of qPCR showed that Foxq1 was differentially expressed in the skin tissues of cashmere goats at E 66 and E 120 stages (P<0.001), which was consistent with the sequencing data. Comet tail fluorescence was observed under fluorescence microscope, indicating that Foxq1 adenovirus was successfully packed, and qPCR results showed that the overexpression efficiency of Foxq1 was more than 40 000 times (P<0.000 1). EdU and MTT results showed that the cell viability of hair follicle stem cells was significantly increased with the overexpression of Foxq1 (P<0.05), and the number of EdU positive cells was significantly increased (P<0.01). Flow cytometry results showed that the percentage of S-phase cells was significantly increased with the overexpression of Foxq1. qPCR results showed that the mRNA expressions of CTNNB1, TCF3 and CYCLIND1 genes were significantly increased with the overexpression of Foxq1 (P<0.05). Immunofluorescence result further demonstrated that the protein expression levels of CTNNB1, TCF3 and CYCLIND1 were significantly increased with the overexpression of Foxq1. This study revealed that Foxq1 could activate the Wnt/β-catenin signaling pathway to promote the proliferation of HFSCs, which illustrated the function and molecular mechanism of Foxq1 in the proliferation of HFSCs.

Key words: Foxq1, HFSCs, Wnt signaling pathway, cell proliferation, cashmere goat

CLC Number:

S827.2

XU Tiantian, ZHANG Tongtong, WANG Meng, WANG Xin. Transcription Factor Foxq1 Affects the Proliferation of Hair Follicle Stem Cells in Cashmere Goats via WNT/β-catenin Signaling Pathway[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2653-2661.

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Transcription Factor Foxq1 Affects the Proliferation of Hair Follicle Stem Cells in Cashmere Goats via WNT/β-catenin Signaling Pathway

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