C型产气荚膜梭菌实验感染仔猪肠道circRNA的表达特征

doi:10.11843/j.issn.0366-6964.2022.11.031

Abstract

Abstract: circRNAs have important regulatory roles in enteric disease development of host infected by pathogenic bacteria. Clostridium perfringens type C (C. perfringens type C) is one of primary bacteria leading to a series of diarrhea and inflammatory diseases in piglets, which caused serious economic losses over the industry. However, the comprehensive and systematic understanding of circRNAs regulating piglet diarrhea caused by C. perfringens type C has not been reported. To illustrate the dynamics of piglet after C. perfringens infection, this study has investigated the expression profiles of ileum circRNAs of 7-day-old piglets challenged by C. perfringens type C through RNA sequencing, screened the differentially expressed circRNAs, and performed the GO and KEGG functional enriched functions. The targets of circRNAs were predicted by miRanda software, then constructing the circRNA/miRNA/mRNA interaction network and finally conducting the qPCR verification. A total of 3 162 circRNAs were identified with quantitative 694 differentially expressed circRNAs in treatment group(TI) and control group(CI) through sequencing, while 404 of which were up-regulated and 290 were down-regulated. Function analysis revealed that these dysregulated circRNAs were mostly enriched in several KEGG pathways including cell cycle, TGF-beta signaling pathway, Lysine degradation, Wnt signaling pathway, T cell receptor signaling pathway, MAPK signaling pathway and so on, regulating piglets resistance response to C. perfringens infection. Furthermore, the interactive network of circRNA-miRNA-mRNA were built in the C. perfringens type C-infected piglet ileum tissues, and the circRNA-associated ceRNA networks composed of 8 circRNAs-5 miRNAs-12 mRNAs was predicted to have important association with CAED. This study may provide valuable information for understanding the regulatory functions of circRNAs of piglet defensed to C. perfringens type C.

Key words: piglet diarrhea, Clostridium perfringenes type C, circRNA, inflammatory response, resistance

CLC Number:

S852.616.3

HUANG Xiaoyu, YANG Qiaoli, YAN Zunqiang, WANG Pengfei, SHI Hairen, GUN Shuangbao. Characterization of circRNA Expression Profiles Involved in Intestines of Clostridium Perfringens Type C-infected Diarrheal Piglet[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 4058-4070.

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Characterization of circRNA Expression Profiles Involved in Intestines of Clostridium Perfringens Type C-infected Diarrheal Piglet

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