基于生物网络功能模块整合组方规律分析四黄止痢颗粒治疗仔猪腹泻的作用机制

doi:10.11843/j.issn.0366-6964.2023.07.034

Abstract

Abstract: The aim of this study was to explore the molecular mechanism of Sihuang Zhili Granule in the treatment of piglet diarrhea based on the functional module, and to explain its compatibility law. TCMSP, TCMID and HERB databases were used to screen the active ingredients of Coptidis Rhizoma, Phellodendri Chinensis Cortex, Rhei Radix et Rhizoma, Scutellariae Radix, Isatidis Radix, Glycyrrhizae Radix et Rhizoma, and collect the related targets. Disease targets of piglet diarrhea were selected from GeneCards、NCBI、OMIM database. Then, the intersection of drug targets and the disease targets were collected by Venny platform, and uploaded to the STRING database for protein interaction analysis. Protein-protein interaction (PPI) network was constructed by Cytoscape software, and its functional module was identified with MCODE plug-in. KEGG pathway enrichment analysis was performed with David database. The results were showed as follows:1) Network analysis showed that a total of 180 active ingredients were obtained, mainly included alkaloids, flavonoids, anthraquinones and glycosides; A total of 88 drug targets and 470 disease targets were screened, and there were 35 intersection targets. 2) The PPI network analysis showed that module 1 composed of 17 target proteins was the functional module of Sihuang Zhili granules. 3) The KEGG enrichment analysis determined that the pathways mainly involved in module 1 include 17 signaling pathways such as TNF, IL-17 and NF-κB. 4) The analysis of meridians showed that Sihuang Zhili granule could act on 11 collateral channels of internal organs of animal body, and mainly on spleen and stomach meridians. In conclusion, alkaloids, flavonoids, anthraquinone and glycosides are the material basis of Sihuang Zhili granules. They mainly regulate the signaling pathways such as TNF, IL-17 and NF-κB by acting on the key targets included in functional module 1, regulate multiple meridians of the body such as spleen and stomach, and play the role of treating piglet diarrhea from multiple levels such as protein targets, signaling pathways and meridians.

Key words: biological network, functional module, Sihuang Zhili granule, piglet diarrhea, mechanism

CLC Number:

S853.9

ZHOU Weiwei, WANG Xuefeng, ZHANG Mengjie, YANG Juan, SUN Yuelong, ZHANG Zufeng, ZHANG Yuxin, DOU Jiahong, WANG Ziying, DAI Xiaofeng, LI Xiumei. Analysing the Mechanism of Sihuang Zhili Granule in the Treatment of Piglet Diarrhea Based on Biological Network Function Modules and Compatibility Rules[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 3031-3043.

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Analysing the Mechanism of Sihuang Zhili Granule in the Treatment of Piglet Diarrhea Based on Biological Network Function Modules and Compatibility Rules

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