基于生物网络功能模块整合组方规律分析四黄止痢颗粒治疗仔猪腹泻的作用机制

doi:10.11843/j.issn.0366-6964.2023.07.034

Abstract

Abstract: The aim of this study was to explore the molecular mechanism of Sihuang Zhili Granule in the treatment of piglet diarrhea based on the functional module, and to explain its compatibility law. TCMSP, TCMID and HERB databases were used to screen the active ingredients of Coptidis Rhizoma, Phellodendri Chinensis Cortex, Rhei Radix et Rhizoma, Scutellariae Radix, Isatidis Radix, Glycyrrhizae Radix et Rhizoma, and collect the related targets. Disease targets of piglet diarrhea were selected from GeneCards、NCBI、OMIM database. Then, the intersection of drug targets and the disease targets were collected by Venny platform, and uploaded to the STRING database for protein interaction analysis. Protein-protein interaction (PPI) network was constructed by Cytoscape software, and its functional module was identified with MCODE plug-in. KEGG pathway enrichment analysis was performed with David database. The results were showed as follows:1) Network analysis showed that a total of 180 active ingredients were obtained, mainly included alkaloids, flavonoids, anthraquinones and glycosides; A total of 88 drug targets and 470 disease targets were screened, and there were 35 intersection targets. 2) The PPI network analysis showed that module 1 composed of 17 target proteins was the functional module of Sihuang Zhili granules. 3) The KEGG enrichment analysis determined that the pathways mainly involved in module 1 include 17 signaling pathways such as TNF, IL-17 and NF-κB. 4) The analysis of meridians showed that Sihuang Zhili granule could act on 11 collateral channels of internal organs of animal body, and mainly on spleen and stomach meridians. In conclusion, alkaloids, flavonoids, anthraquinone and glycosides are the material basis of Sihuang Zhili granules. They mainly regulate the signaling pathways such as TNF, IL-17 and NF-κB by acting on the key targets included in functional module 1, regulate multiple meridians of the body such as spleen and stomach, and play the role of treating piglet diarrhea from multiple levels such as protein targets, signaling pathways and meridians.

Key words: biological network, functional module, Sihuang Zhili granule, piglet diarrhea, mechanism

CLC Number:

S853.9

ZHOU Weiwei, WANG Xuefeng, ZHANG Mengjie, YANG Juan, SUN Yuelong, ZHANG Zufeng, ZHANG Yuxin, DOU Jiahong, WANG Ziying, DAI Xiaofeng, LI Xiumei. Analysing the Mechanism of Sihuang Zhili Granule in the Treatment of Piglet Diarrhea Based on Biological Network Function Modules and Compatibility Rules[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 3031-3043.

References 59

[1]	云巾宴, 尹柏双, 呼显生, 等.中药作为替抗产品在病毒性仔猪腹泻中的研究现状及应用前景[J].饲料研究, 2021, 44(8):143-146.YUN J Y, YIN B S, HU X S, et al.Application prospect of Chinese medicine as a substitute product in preventing and treating viral piglet diarrhea[J].Feed Research, 2021, 44(8):143-146.(in Chinese)
[2]	LIU G, GUAN G P, FANG J, et al.Macleaya cordata extract decreased diarrhea score and enhanced intestinal barrier function in growing piglets[J].Biomed Res Int, 2016, 5(6):1069585.
[3]	LIU C, ZHANG C, LV W J, et al.Structural modulation of gut microbiota during alleviation of suckling piglets diarrhoea with herbal formula[J].Evid-Based Compl Alt, 2017, 10(12):8358151.
[4]	刘辉.中药组方对猪大肠杆菌病抑菌效果观察[J].中国畜禽种业, 2017, 13(5):101.LIU H.Observation on bacteriostasis effect of traditional Chinese medicine prescription on pig Escherichia coli disease[J].The Chinese Livestock and Poultry Breeding, 2017, 13(5):101.(in Chinese)
[5]	郭大伟, 侯思鲁, 池宇佳, 等.芪英汤和子甘汤中药复方促进母猪繁殖性能和断奶仔猪生长性能的临床研究[J].畜牧兽医学报, 2022, 53(6):1994-2004.GUO D W, HOU S L, CHI Y J, et al.Clinical research of Qiying decoction and Zigan decoction on promoting reproductive performance of sows and growth performance of weaned piglets[J].Acta Veterinaria et Zootechnica Sinica, 2022, 53(6):1994-2004.(in Chinese)
[6]	中国兽药典委员会.中华人民共和国兽药典-二部[S].北京:中国农业出版社, 2016.Commission of Chinese Veterinary Pharmacopoeia.Chinese veterinary pharmacopoeia-Part Ⅱ[S].Beijing:China Agriculture Press, 2016.(in Chinese)
[7]	蒋红, 王宏军, 周铁忠, 等.四黄止痢颗粒中六味中药相互配伍对鸡大肠杆菌的作用研究[J].中国农学通报, 2012, 28(14):89-92.JIANG H, WANG H J, ZHOU T Z, et al.The combined antibacterial effect study of six herbs in Sihuang Zhili granules against chicken E.coli[J].Chinese Agricultural Science Bulletin, 2012, 28(14):89-92.(in Chinese)
[8]	黄楠, 邱子雯, 黄立层, 等.四黄止痢颗粒不同组分联合对多杀性巴氏杆菌的体外抑制作用研究[J].中兽医医药杂志, 2019, 38(1):19-22.HUNG N, QIU Z W, HUANG L C, et al.Antibacterial effects of different compounds of herbal medicine from Sihuangzhili granules against Pasteurella multocida in vitro[J].Journal of Traditional Chinese Veterinary Medicine, 2019, 38(1):19-22.(in Chinese)
[9]	董朕, 白东东, 刘利利, 等.基于网络药理学分析归芪益母汤治疗牛气血两虚证的作用机制[J].畜牧兽医学报, 2018, 49(12):2733-2744.DONG Z, BAI D D, LIU L L, et al.The mechanism of Gui Qi Yimu decoction powder in treating cow qi and blood two deficiency syndrome based on network pharmacology[J].Acta Veterinaria et Zootechnica Sinica, 2018, 49(12):2733-2744.(in Chinese)
[10]	陈寅萤, 王忠, 南景一, 等.丹红注射液治疗冠心病心绞痛基因网络的模块划分与生物学机制分析[J].中国实验方剂学杂志, 2020, 26(12):185-194.CHEN Y Y, WANG Z, NAN J Y, et al.Analysis of modular network mechanism of Danhong injection in treatment of coronary angina pectoris[J].Chinese Journal of Experimental Traditional Medical Formulae, 2020, 26(12):185-194.(in Chinese)
[11]	BADER G D, HOGUE C W.An automated method for finding molecular complexes in large protein interaction networks[J].BMC Bioinformatics, 2003, 13:2.
[12]	WANG Z, LIU J, YU Y N, et al.Modular pharmacology:the next paradigm in drug discovery[J].Expert Opin Drug Dis, 2012, 7(8):667-677.
[13]	WANG Z, WANG Y Y.Modular pharmacology:deciphering the interacting structural organization of the targeted networks[J].Drug Discov Today, 2013, 18(11-12):560-566.
[14]	刘思鸿, 赵汉青, 高宏杰, 等.一种基于中医"君臣佐使"理论的加权网络模块划分优化方法[J].中国中药杂志, 2021, 46(22):5936-5943.LIU S H, ZHAO H Q, GAO H J, et al.An optimization method of weighted network module partition based on TCM theory of "monarch, minister, assistant and guide"[J].China Journal of Chinese Materia Medica, 2021, 46(22):5936-5943.(in Chinese)
[15]	田智勇, 李振国.黄连的研究新进展[J].时珍国医国药, 2004, 15(10):704-706.TIAN Z Y, LI Z G.Research progress of Rhizoma Coptidis[J].Lishizhen Medicine and Materia Medica Research, 2004, 15(10):704-706.(in Chinese)
[16]	林媛, 司书毅, 蒋建东.小檗碱的抗菌作用[J].药学学报, 2018, 53(2):163-168.LIN Y, SI S Y, JIANG J D.Antibacterial activity of berberine[J].Acta Pharmaceutica Sinica, 2018, 53(2):163-168.(in Chinese)
[17]	周剑雄, 吴送姑, 龚俊波, 等.小檗碱的药理活性以及提升其口服生物利用度的策略[J].药学学报, 2022, 57(5):1263-1272.ZHOU J X, WU S G, GONG J B, et al.Pharmacological activities of berberine and strategies to improve its oral bioavailability[J].Acta Pharmaceutica Sinica, 2022, 57(5):1263-1272.(in Chinese)
[18]	YU M K, JIN X J, LIANG C H, et al.Berberine for diarrhea in children and adults:a systematic review and meta-analysis[J].Ther Adv Gastroenter, 2020, 13:1-19.
[19]	DUBREUIL J D.Antibacterial and antidiarrheal activities of plant products against enterotoxinogenic Escherichia coli[J].Toxins (Basel), 2013, 5(11):2009-2041.
[20]	杨勇, 叶小利, 李学刚.4种黄连生物碱的抑菌作用[J].时珍国医国药, 2007, 18(12):3013-3014.YANG Y, YE X L, LI X G.Antimicrobial effect of four alkaloids from Coptidis Rhizome[J].Lishizhen Medicine and Materia Medica Research, 2007, 18(12):3013-3014.(in Chinese)
[21]	李峰, 张浩, 华桦, 等.黄连碱对应激所致小鼠胃黏膜损伤的保护作用[J].华西药学杂志, 2007, 22(6):713-714.LI F, ZHANG H, HUA H, et al.Protective effect of coptisine on gastric mucosa injury induced by stress in mice[J].West China Journal of Pharmaceutical Sciences, 2007, 22(6):713-714.(in Chinese)
[22]	王道武, 邵凯, 张龙.小檗碱和小檗红碱13位衍生物的合成[J].广东化工, 2010, 37(3):118-120.WANG D W, SHAO K, ZHANG L.Synthesis of 13-(substituted benzyl) berberine and berberrubine derivatives[J].Guangdong Chemical Industry, 2010, 37(3):118-120.(in Chinese)
[23]	PARK K D, LEE J H, KIM S H, et al.Synthesis of 13-(substituted benzyl) berberine and berberrubine derivatives as antifungal agents[J].Bioorg Med Chem Lett, 2006, 16(15):3913-3916.
[24]	李嘉诚, 吴岚, 蔡同凯, 等.黄柏化学成分及其药理作用研究进展[J].药学实践杂志, 2018, 36(5):389-391, 398.LI J C, WU L, CAI T K, et al.Research progress of Cortex Phellodendri in the chemical constituents and their pharmacological effects[J].Journal of Pharmaceutical Practice, 2018, 36(5):389-391, 398.(in Chinese)
[25]	FUJII A, OKUYAMA T, WAKAME K, et al.Identification of anti-inflammatory constituents in Phellodendri Cortex and Coptidis Rhizoma by monitoring the suppression of nitric oxide production[J].J Nat Med, 2017, 71(4):745-756.
[26]	JUNG H W, KIM K H, PARK Y K.Inhibitory effect of the extract of Phellodendron amurense ruprecht root on collagen-induced arthritis in mice[J].Chin J Integr Med, 2017, 23(10):755-762.
[27]	龚发萍, 郑鸣.黄芩的化学成分及药理作用[J].临床合理用药杂志, 2021, 14(34):176-178.GONG F P, ZHENG M.Chemical constituents and pharmacological effects of Scutellaria baicalensis Georgi[J].Chinese Journal of Clinical Rational Drug Use, 2021, 14(34):176-178.(in Chinese)
[28]	王亦君, 冯舒涵, 程锦堂, 等.大黄蒽醌类化学成分和药理作用研究进展[J].中国实验方剂学杂志, 2018, 24(13):227-234.WANG Y J, FENG S H, CHENG J T, et al.Research progress on chemical constituents and pharmacological action of Rheum anthraquinone[J].Chinese Journal of Experimental Traditional Medical Formulae, 2018, 24(13):227-234.(in Chinese)
[29]	郭向华, 周联, 王青, 等.芦荟大黄素对结肠炎小鼠模型脾脏T细胞亚群的影响[J].中药新药与临床药理, 2013, 24(4):356-360.GUO X H, ZHOU L, WANG Q, et al.Effects of aloe-emodin on splenic T cell subsets in mice with colitis[J].Traditional Chinese Drug Research and Clinical Pharmacology, 2013, 24(4):356-360.(in Chinese)
[30]	罗爽, 罗霞, 刘琦, 等.大黄酸对DSS诱导溃疡性结肠炎小鼠的治疗作用及机制探讨[J].中国实验方剂学杂志, 2017, 23(11):109-113.LUO S, LUO X, LIU Q, et al.Effect and mechanism of rhein on DSS-induced ulcerative colitis in mice[J].Chinese Journal of Experimental Traditional Medical Formulae, 2017, 23(11):109-113.(in Chinese)
[31]	邓九零, 陶玉龙, 何玉琼, 等.板蓝根抗流感病毒活性成分及其作用机制研究进展[J].中国中药杂志, 2021, 46(8):2029-2036.DENG J L, TAO Y L, HE Y Q, et al.Research progress on active components and mechanism of Isatidis Radix for influenza virus[J].China Journal of Chinese Materia Medica, 2021, 46(8):2029-2036.(in Chinese)
[32]	张洪江, 何立巍, 唐敏.板蓝根中黄酮类化合物抗流感病毒的分子对接研究[J].化工时刊, 2018, 32(3):19-21.ZHANG H J, HE L W, TANG M.Study on the anti-influenza effect of the flavonoids in Radix isatidis through Molecular docking[J].Chemical Industry Times, 2018, 32(3):19-21.(in Chinese)
[33]	贾世亮, 武雪玲, 李筱筱, 等.甘草中黄酮类物质的功能研究进展[J].北京联合大学学报, 2016, 30(4):67-73.JIA S L, WU X L, LI X X, et al.Research progress of the functions of licorice flavonoids[J].Journal of Beijing Union University, 2016, 30(4):67-73.(in Chinese)
[34]	SHIM J M, KIM J, TENSON T, et al.Influenza virus infection, interferon response, viral counter-response, and apoptosis[J].Viruses, 2017, 9(8):223.
[35]	邓东沅, 顾立刚, 刘晓婷, 等.木犀草素体外对H1N1感染A549诱导凋亡的干预作用及机制[J].中华中医药杂志, 2017, 32(4):1524-1527.DENG D Y, GU L G, LIU X T, et al.Luteolin's intervention effect and its mechanism of apoptosis induced by H1N1 in vitro[J].China Journal of Traditional Chinese Medicine and Pharmacy, 2017, 32(4):1524-1527.(in Chinese)
[36]	于卓男, 林树鹏, 顾立刚, 等.流感病毒H1N1感染A549细胞诱导凋亡及川芎嗪干预作用的研究[J].中华中医药杂志, 2016, 31(7):2815-2817.YU Z N, LIN S P, GU L G, et al.Effects and mechanism of ligustrazine on apoptosis of A549 cells induced by H1N1 virus in vitro[J].China Journal of Traditional Chinese Medicine and Pharmacy, 2016, 31(7):2815-2817.(in Chinese)
[37]	杨雨薇, 房希碧, 于海滨, 等.奶牛乳腺上皮细胞中Bta-miR-877靶基因的初步验证[J].中国兽医学报, 2018, 38(4):809-813.YANG Y W, FANG X B, YU H B, et al.Validation of Bta-miR-877 target genes in bovine mammary epithelial cells[J].Chinese Journal of Veterinary Science, 2018, 38(4):809-813.(in Chinese)
[38]	CHIU J W, HANAFI Z B, CHEW L C Y, et al.IL-1α processing, signaling and its role in cancer progression[J].Cells, 2021, 10(1):92.
[39]	MACSHARRY J, O'MAHONY L, FANNING A, et al.Mucosal cytokine imbalance in irritable bowel syndrome[J].Scand J Gastroenterol, 2008, 43(12):1467-1476.
[40]	MAZAR A P, HENKIN J, GOLDFARB R H.The urokinase plasminogen activator system in cancer:implications for tumor angiogenesis and metastasis[J].Angiogenesis, 1999, 3(1):15-32.
[41]	WOLFF C, MALINOWSKY K, BERG D, et al.Signalling networks associated with urokinase-type plasminogen activator (uPA) and its inhibitor PAI-1 in breast cancer tissues:new insights from protein microarray analysis[J].J Pathol, 2011, 223(1):54-63.
[42]	龚震宇, 刘顺财.一氧化氮与肠道炎症关系的研究进展[J].现代消化及介入诊疗, 2020, 25(1):136-138.GONG Z Y, LIU S C.Research progress on the relationship between nitric oxide and intestinal inflammation[J].Modern Digestion & Intervention, 2020, 25(1):136-138.(in Chinese)
[43]	王春梅, 李贺, 吴金滢.黄连素调节eNOS/NO保护软脂酸诱导的HUVECs损伤作用机制研究[J].北华大学学报(自然科学版), 2014, 15(6):743-746.WANG C M, LI H, WU J Y.Protective effects of berberine on palmitate-induced injury in HUVECs by regulating eNOS/NO[J].Journal of Beihua University (Natural Science), 2014, 15(6):743-746.(in Chinese)
[44]	靳枫, 余少康, 李柱, 等.胃癌患者血清miR-183 E-selectin AMBP的变化及与临床病理的关系[J].河北医学, 2019, 25(12):2094-2098.JIN F, YU S K, LI Z, et al.Changes of serum miR-183, E-selectin and AMBP in patients with gastric cancer and their relationship with clinical pathology[J].Hebei Medicine, 2019, 25(12):2094-2098.(in Chinese)
[45]	BRUEY J M, BRUEY-SEDANO N, LUCIANO F, et al.Bcl-2 and Bcl-XL regulate proinflammatory caspase-1 activation by interaction with NALP1[J].Cell, 2007, 129(1):45-56.
[46]	LIN F C, YOUNG H A.Interferons:success in anti-viral immunotherapy[J].Cytokine Growth Fact Rev, 2014, 25(4):369-376.
[47]	IDRISS H T, NAISMITH J H.TNFα and the TNF receptor superfamily:structure-function relationship(s)[J].Microsc Res Techniq, 2000, 50(3):184-195.
[48]	HERMANS L, DE PELSMAEKER S, DENAEGHEL S, et al.β-glucan-induced IL-10 secretion by monocytes triggers porcine NK cell cytotoxicity[J].Front Immunol, 2021, 12:634402.
[49]	KANG S J, NARAZAKI M, METWALLY H, et al.Historical overview of the interleukin-6 family cytokine[J].J Exp Med, 2020, 217(5):e20190347.
[50]	CHEN M Y, LIANG X L, LIANG Z Q, et al.Study on the effect and mechanism of NFKBIA on cervical cancer progress in vitro and in vivo[J].J Obstet Gynaecol Res, 2021, 47(11):3931-3942.
[51]	邱倩.草鱼原癌基因c-fos的原核表达载体构建及蛋白表达纯化[J].陇东学院学报, 2022, 33(2):105-109.QIU Q.Construction of prokaryotic expression vector and protein purification of proto-oncogene c-fos of Ctenopharyngodon idellus[J].Journal of Longdong University, 2022, 33(2):105-109.(in Chinese)
[52]	成豫, 杨文霞, 汪珏楚.猪繁殖与呼吸综合症病毒与血红素加氧酶-1的研究进展[J].畜禽业, 2021, 32(7):14, 16.CHENG Y, YANG W X, WANG J C.Research progress of porcine reproductive and respiratory syndrome virus and Heme oxygenase-1[J].Livestock and Poultry Industry, 2021, 32(7):14, 16.(in Chinese)
[53]	FISCHER R, KONTERMANN R E, PFIZENMAIER K.Selective targeting of TNF receptors as a novel therapeutic approach[J].Front Cell Dev Biol, 2020, 8:401.
[54]	GILMORE T D.Introduction to NF-κB:players, pathways, perspectives[J].Oncogene, 2006, 25(51):6680-6684.
[55]	LI J B, MA J, WANG K S, et al.Baicalein inhibits TNF-α-induced NF-κB activation and expression of NF-κB-regulated target gene products[J].Oncol Rep, 2016, 36(5):2771-2776.
[56]	SHI H, MA J, MI C L, et al.Amorfrutin a inhibits TNF-α-induced NF-κB activation and NF-κB-regulated target gene products[J].Int Immunopharmacol, 2014, 21(1):56-62.
[57]	刘诗聪, 范颖, 梁茂新.论方药归经的分类特征与融合[J].世界科学技术-中医药现代化, 2021, 23(3):778-784.LIU S C, FAN Y, LIANG M X.A discussion on the classification characteristics and integration of the meridian tropism of prescriptions and medicinals[J].Modernization of Traditional Chinese Medicine and Materia Materia-World Science and Technology, 2021, 23(3):778-784.(in Chinese)
[58]	郑举, 刘红云, 陈静, 等.HPLC波长切换法同时测定四黄止痢颗粒中5种成分的含量[J].中兽医医药杂志, 2020, 39(3):58-62.ZHENG J, LIU H Y, CHEN J, et al.Simultaneous determination of five constituents in Sihuangzhili granules by HPLC with switching wavelength[J].Journal of Traditional Chinese Veterinary Medicine, 2020, 39(3):58-62.(in Chinese)
[59]	刘昌孝, 陈士林, 肖小河, 等.中药质量标志物(Q-Marker):中药产品质量控制的新概念[J].中草药, 2016, 47(9):1443-1457.LIU C X, CHEN S L, XIAO X H, et al.A new concept on quality marker of Chinese materia medica:Quality control for Chinese medicinal products[J].Chinese Traditional and Herbal Drugs, 2016, 47(9):1443-1457.(in Chinese)

Analysing the Mechanism of Sihuang Zhili Granule in the Treatment of Piglet Diarrhea Based on Biological Network Function Modules and Compatibility Rules

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