NAC通过抑制大鼠肺细胞凋亡缓解镉致肺组织损伤

doi:10.11843/j.issn.0366-6964.2022.09.038

Abstract

Abstract: The aim of this study was to evaluate the alleviation effect of N-acetyl-L-cysteine (NAC) on cadmium (Cd) exposure-induced the apoptosis of lung cells and the damage of lung tissue in the ovariectomized rats. Forty adult female Sprague-Dawley (SD) rats were randomly divided into sham operation group (Control), Cd group, NAC group (NAC), and NAC+Cd group (NAC+Cd). Each group contained 10 rats. After feeding for 18 months, the lung tissue of the rat was separated for further tests. The pathological change of lung tissue was observed after the HE staining and the Masson staining. The content of Cd in lung tissue was detected by atomic absorption analyzer. The mRNA levels of lung tissue fibrotic key proteins (Col-I and Col-III) were analyzed by the qRT-PCR. In addition, the expression of key apoptosis proteins (Bcl-2, Bax, and cleaved caspase-3) and p53, and Akt phosphorylation were evaluated by Western blot. The results showed that the disappearance of alveolar structure and the hyperplasia of fibrous tissue, the content of Cd was significantly (P<0.01) increased, the mRNA levels of Col-I and Col-III were significantly (P<0.01) up-regulated, and the ratio of Bax/Bcl-2, the expression of cleaved caspase-3 and p53 were increased in lung tissue of Cd group compared with Control group, but the phosphorylation of Akt was inhibited. The pathological change, the content of Cd, the mRNA levels of Col-I and Col-III, and the ratio of Bax/Bcl-2, the expression of cleaved caspase-3 and p53 were decreased in lung tissue of NAC+Cd group compared with Cd group, but the phosphorylation of Akt was increased. These results indicated that NAC has a certain alleviating effect on Cd-induced the damage of lung tissue and the apoptosis of lung cells in rats.

Key words: cadmium (Cd), N-acetyl-L-cysteine (NAC), lung tissue, rats, apoptosis

CLC Number:

S856.9

TONG Xishuai, YU Gengsheng, LI Yawen, RAN Di, GU Jianhong, ZOU Hui, YUAN Yan, BIAN Jianchun, LIU Zongping. NAC Alleviates Cadmium-induced Lung Damage by Inhibiting the Apoptosis of Lung Cell in Rats[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3241-3250.

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NAC Alleviates Cadmium-induced Lung Damage by Inhibiting the Apoptosis of Lung Cell in Rats

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