GPx6在大白猪附睾细胞中的表达及其与繁殖性能的相关性

doi:10.11843/j.issn.0366-6964.2021.09.011

Abstract

Abstract: This study aimed to investigate the location and expression of glutathione peroxidase 6(GPx6) in epididymal cells of Large White boars as well to explore their correlation with reproductive performance of boars. Three female and male Large White pigs at 15 months old were selected, respectively. Testis, epididymis, prostate, urethral bulb gland, seminal vesicle, ovary and fallopian tube were collected for protein extraction. Expression and localization of GPx6 protein in different tissues and cells were detected by Western blot and immunohistochemistry (IHC). Boar fertilization ability was evaluated based on a mathematical mode, according to the standard of breeding parity ≥ 20 births and 3 times of breeding semen from the same boar, 20 Large White boars were selected along with their semen collected. Further, productive performance data of the 1 279 boar-paired sows were collected for evaluating the reproductive performance of those 20 breeding boars including total number born per litter (TNB), number born alive per litter (NBA), farrowing rate (FR) and fecundity. Protein from semen and sperm samples were extracted, BCA and ELISA were carried on to detect GPx6 content in sperm and seminal plasma. SPSS software was used for data statistics, while independent sample t-Test and one-way ANOVA were carried out for significant difference analysis, bivariate Pearson was used for correlation analysis, and P<0.05 indicated significant difference or correlation. The results indicated that GPx6 protein was highly expressed in epididymis, and IHC results showed GPx6 was expressed in apical cells, basal cells, halo cells, principal cells and spermatozoa from epididymis whereas was not expressed in myoid cells. The concentration of GPx6 protein in seminal plasma was as 7 times higher than that in sperm, meanwhile, the concentration of GPx6 protein in semen was negatively correlated with NBA and TNB. This study suggests that GPx6 is expressed in apical cells, basal cells, halo cells, principal cells and spermatozoa of epididymis, and its content in semen affects reproductive performance of boars, which provides a theoretical and experimental foundation for the research of function of GPx6 protein in boar fertility.

Key words: boar, epididymis, reproductive performance, glutathione peroxidase 6

CLC Number:

S813.83

CHEN Yun, WANG Kai, ZHOU Lele, WEI Hengxi, LI Li, ZHANG Shouquan. Expression of GPx6 in Epididymal Cells of Large White Boar and Its Correlation with Reproductive Performance[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2484-2490.

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Expression of GPx6 in Epididymal Cells of Large White Boar and Its Correlation with Reproductive Performance

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