N-乙酰半胱氨酸对山羊子宫内膜基质细胞增殖及相关基因表达的影响

doi:10.11843/j.issn.0366-6964.2022.01.014

Abstract

Abstract: The aim was to explore the effect of N-acetyl-L-cysteine (NAC) on goat endometrial stromal cells (gESCs). In this study, goat endometrial stromal cells were used as the object. Three concentration gradients of 100,200,400 μmol·L^-1 NAC were added to the culture medium. The 0 μmol·L^-1 NAC was regarded as the blank group and CCK -8 method was used to screen the optimal concentration of NAC and judge its effect on cell proliferation.Then DCFH-DA probe and RT-qPCR were used to detect the optimal concentration of NAC on the intracellular reactive oxygen content, cell cycle and proliferation related factors, and goat reproductive performance-related genes TGF-β1 and TGF-β3 mRNA expression levels. The results showed that the concentration of 200 μmol·L^-1 NAC had a more significant effect on the proliferation of goat endometrial stromal cells than other gradients. In addition, 200 μmol·L^-1 NAC reduced the ROS content of cells compared with the blank group(P<0.01), thereby delaying cell senescence. RT-qPCR results showed that the addition of 200 μmol·L^-1 NAC significantly increased the expression levels of cell cycle and proliferation-related factors CyclinA1, CyclinE, PCNA mRNA (P<0.01), and reduced the mRNA expression of cell cycle factor CyclinD2(P<0.01); and extremely significantly reduced the TGF-β1 gene(P<0.01) of the transforming growth factor family and significantly reduced the mRNA expression level of the TGF-β3 gene(P<0.05). Therefore, this study speculates that 200 μmol·L^-1 NAC may promote cell proliferation by regulating the expression levels of CyclinA1, CyclinE, PCNA, and CyclinD2 in cells, and affect goat reproduction traits by regulating the expression levels of transforming growth family factors TGF-β1 and TGF-β3. This study provide a theoretical basis for further research on the mechanism of NAC’s influence on goat reproductive traits.

Key words: N-acetylcysteine, gESCs, cell proliferation, gene expression, reproductive performance

CLC Number:

S827.3

YANG Peifang, CHEN Xiang, ZHOU Zhinan, TANG Wen, ZHANG Yan, HUI Maomao. The Effect of N-acetylcysteine on Proliferation and Related Gene Expression of Goat Endometrial Stromal Cells in vitro[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 141-150.

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The Effect of N-acetylcysteine on Proliferation and Related Gene Expression of Goat Endometrial Stromal Cells in vitro

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