体细胞克隆技术在乌金猪遗传资源保护上的应用

doi:10.11843/j.issn.0366-6964.2020.09.010

Abstract

Abstract: The purpose of this experiment was to study the growth and developmental ability and reproductive performance of cloned Wujin pigs fire hair line by somatic cell cloning technology, and provide a theoretical basis for the application of somatic cell cloning technology in the conservation of local excellent pig breeds. In this study, the ear samples were collected from 3 male and 12 female pigs which met the characteristics of Wujin pigs. The 3 boars were 3, 6 and 11 months old, respectively. The sows had a large age gap, from 2 months old to 10 years old, the three sows and one boar of 3-month-old had been castrated and the sow of 10-year-old had no reproductive capacity. The ear fibroblast cell lines were successfully established from 15 Wujin pigs. Then the fibroblast cells from 3-month-old(♂) and 10-year-old(♀) pigs were used as donor cells for somatic cell nuclear transfer. The cloned embryos were transplanted into 16 surrogate sows and 25 cloned pigs were obtained. The growth and development indexes of these cloned pigs were all normal. After sexual maturity, the male and female cloned pigs mated naturally and 39 live piglets (F1) were obtained. This study successfully cloned Wujin pigs by somatic cell nuclear transfer technology, and cloned Wujin pigs had normal growth and developmental ability and reproductive performance. The study result provides a new way for the conservation of local pig breeds.

Key words: Wujin pigs, somatic cell cloning technology, growth performance, reproductive performance

CLC Number:

S828.3

MAO Shengyi, LI Zhuo, KUI Hua, JIAO Deling, LI Yuying, QING Yubo, GUO Jianxiong, WEI Yunfang, WEI Hongjiang. Application of Somatic Cell Cloning Technology in the Protection of Genetic Resources of Wujin Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(9): 2130-2137.

References 27

[1]	郭海涛,郭成裕,李军鹏,等.我国地方猪品种-乌金猪的生理特性研究[J].现代畜牧兽医,2008(10):17-18.GUO H T,GUO C Y,LI J P,et al.Studies on physiological characteristics of Wujin pig in domestic local pig breeds[J].Modern Journal of Animal Husbandry and Veterinary Medicine,2008(10):17-18.(in Chinese)
[2]	王冶,郭荣富.乌金猪研究进展[J].黑龙江畜牧兽医,2017(10):90-92.WANG Y,GUO R F.Recent advances of Wujin pig[J].Heilongjiang Animal Science and Veterinary Medicine,2017(10):90-92.(in Chinese)
[3]	雷怀刚,杨润林,沈静,等.乌金猪(凉山猪)种质特性概述[J].中国猪业,2013,8(S1):149-150.LEI H G,YANG R L,SHEN J,et al.Characteristic of Wujin (Liangshan) pig[J].China Swine Industry,2013,8(S1):149-150.(in Chinese)
[4]	国家畜禽遗传资源委员会.中国畜禽遗传资源志-猪志[M].北京:中国农业出版社,2011.China National Commission of Animal Genetic Resources.Animal genetic resources in China-Pigs[M].Beijing:China Agriculture Press, 2011.(in Chinese)
[5]	王忠庆,荣耀先.大河乌猪与大河猪(乌金猪)肉质性状研究[J].云南农业科技,2004(S1):41-44.WANG Z Q,RONG Y X.Study on the meat characteristics of Dahe Swine (Wujin Swine)[J].Yunnan Agricultural Science and Technology,2004(S1):41-44.(in Chinese)
[6]	鲁绍雄,连林生,李庆科,等.基于乌金猪深度发掘的优质高效新品种选育[J].猪业科学,2016,33(12):120-122.LU S X,LIAN L S,LI Q K,et al.Breeding of new variety based on Wujin pig[J].Swine Industry Science,2016, 33(12):120-122.(in Chinese)
[7]	尤如华,余宗寿.乌金猪保种选育与开发利用研究进展[J].中国猪业,2013,8(S1):173-174.YOU R H,YU Z S.Research progress on maintenance,selection and development of Wujin pig resources[J].China Swine Industry,2013,8(S1):173-174.(in Chinese)
[8]	黄卫平,王强,黄剑锋,等.乌金猪品种资源及开发现状[J].四川畜牧兽医,2017,44(3):42-43.HUANG W P,WANG Q,HUANG J F,et al.Current status and development of Wujin pig resources[J].Sichuan Animal & Veterinary Sciences,2017,44(3):42-43.(in Chinese)
[9]	阎景娟,马月辉,道尔吉.浅析畜禽活体保种的影响因素[J].动物科学与动物医学,2003,20(5):63-65.YAN J J,MA Y H,DAO E J.The factors livestock living bodies conservation[J].Animal Science & Veterinary Medicine,2003, 20(5):63-65.(in Chinese)
[10]	甘麦邻,杨露,蒲红州,等.畜禽保种理论与方法在猪保种工作中的应用[J].猪业科学,2017,34(3):127-130.GAN M L,YANG L,PU H Z,et al.The application of theory and method for livestock conservation in pig[J].Swine Industry Science,2017,34(3):127-130.(in Chinese)
[11]	鲁立刚,鲁绍雄.影响家畜原位保种效果的主要因素研究[J].黑龙江畜牧兽医,2009(5):45-47.LU L G,LU S X.Study on the major factors in situ conservation of domestic animals[J].Heilongjiang Animal Science and Veterinary Medicine,2009(5):45-47.(in Chinese)
[12]	JOHNSON L A,WEITZE K F,FISER P,et al.Storage of boar semen[J].Anim Reprod Sci,2000,62(1-3):143-172.
[13]	PARKS J E.Hypothermia and mammalian gametes[M].San Diego:Academic Press,1997:229-261.
[14]	朱士恩.猪精液冷冻技术研究[J].猪业科学,2007,24(5):44-47.ZHU S E.Technology of freezing pig semen[J].Swine Industry Science,2007,24(5):44-47.(in Chinese)
[15]	PEZO F,ROMERO F,ZAMBRANO F,et al.Preservation of boar semen:an update[J].Reprod Domest Anim,2019,54(3):423-434.
[16]	ZHANG W,YI K L,YAN H F,et al.Advances on in vitro production and cryopreservation of porcine embryos[J].Anim Reprod Sci,2012,132(3-4):115-122.
[17]	叶雷,李红,魏红江,等.成年版纳微型猪近交系克隆猪的制备[J].畜牧兽医学报,2012,43(9):1491-1498.YE L,LI H,WEI H J,et al.Production of cloning adult Banna miniature inbred pig[J].Acta Veterinaria et Zootechnica Sinica, 2012,43(9):1491-1498.(in Chinese)
[18]	YU H H,ZHAO H,QING Y B,et al.Porcine zygote injection with Cas9/sgRNA results in DMD-modified pig with muscle dystrophy[J].Int J Mol Sci,2016,17(10):1668.
[19]	CHENG W M,ZHAO H,YU H H,et al.Efficient generation of GGTA1-null Diannan miniature pigs using TALENs combined with somatic cell nuclear transfer[J].Reprod Biol Endocrinol,2016,14(1):77.
[20]	SHEN Y F,XU K X,YUAN Z M,et al.Efficient generation of P53 biallelic knockout Diannan miniature pigs via TALENs and somatic cell nuclear transfer[J].J Transl Med,2017,15(1):224.
[21]	YU H H,LONG W H,ZHANG X Z,et al.Generation of GHR-modified pigs as Laron syndrome models via a dual-sgRNAs/Cas9 system and somatic cell nuclear transfer[J].J Transl Med,2018,16(1):41.
[22]	NIU D,WEI H J,LIN L,et al.Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9[J]. Science, 2017, 357(6357):1303-1307.
[23]	甘丽娜,钦伟云,杨建生,等.不同月龄中梅山猪体尺体质量及其生长增量的测定分析[J].江苏农业科学,2017,45(13):133-135.GAN L N,QIN W Y,YANG J S,et al.Measurement and analysis of body size and growth increment of Meishan pig in different months[J].Jiangsu Agricultural Sciences,2017,45(13):133-135.(in Chinese)
[24]	LEIBO S P,SZTEIN J M.Cryopreservation of mammalian embryos:Derivation of a method[J].Cryobiology,2019,86:1-9.
[25]	王献伟.非洲猪瘟背景下种猪资源生物保种策略研究[J].河南畜牧兽医,2019,40(10):5-6.WANG X W.Study on biological conservation strategy of swine resources in the context of African swine fever[J].Henan Journal of Animal Husbandry and Veterinary Medicine,2019,40(10):5-6.(in Chinese)
[26]	WONGTAWAN T,SARAVIA F,WALLGREN M.Fertility after deep intra-uterine artificial insemination of concentrated low-volume boar semen doses[J].Theriogenology,2006,65(4):773-787.
[27]	魏红江, 王文,卿玉波,等.一种利用体细胞克隆技术培育地方优良家畜品种的方法[P].CN106755112B,2019-07-12.WEI H J, WANG W, QING Y B, et al. Method for cultivating excellent local livestock breeds using somatic cell cloning technology[P]. CN106755112B, 2019-07-12.(in Chinese)

Application of Somatic Cell Cloning Technology in the Protection of Genetic Resources of Wujin Pigs

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