免疫细胞在牦牛附睾和输精管的分布规律

doi:10.11843/j.issn.0366-6964.2022.06.026

Abstract

Abstract: This paper aimed to study the distribution characteristics of immune cells in the epididymis and vas deferens of healthy yaks. The location of CD68⁺ macrophages, CD3⁺ T lymphocytes, CD79α⁺ B lymphocytes, IgA⁺ and IgG⁺ plasmocytes, and the expression of surface markers of immune cells in the epididymis (head, body and tail) and vas deferens of juvenile (5-6 months old) and adult (3-4 years old) yaks were detected by immunohistochemistry and real-time fluorescence quantitative (qRT-PCR). The results showed that CD68⁺ macrophages, CD3⁺ T lymphocytes, CD79α⁺ B lymphocytes, IgA⁺ and IgG⁺ plasmocytes were mainly distributed in the epithelium and interstitium of the epididymis and vas deferens. Additionally the CD68 and CD3 mRNA and protein level in the caput and corpus of yaks were significantly higher than that of the cauda and vas deferens (P < 0.05), while in the cauda and vas deferens CD79α, IgA and IgG mRNA and protein level were significantly higher than those of the caput and corpus epididymis (P < 0.05). Moreover the CD68, CD3, CD79α, IgA and IgG mRNA and protein level in the epididymis and vas deferens of the adult yaks were significantly higher than those of the juvenile yaks (P < 0.05). The above results suggest that the caput may be the main site of cellular immunity, while the caudal epididymis and vas deferens are the main area of humoral immune response. Moreover the local immunity of the epididymis and vas deferens of the yak may mature and expand to a fully functional state in adult yaks. The above data would provide morphological data for further research on local reproductive immunoregulation and pathology in plateau yaks.

Key words: yak, epididymis, vas deferens, immune cells

CLC Number:

S852.16

ZHANG Qian, CUI Yan, YU Sijiu, HE Junfeng, PAN Yangyang, WANG Meng. Distribution of Immune Cells in the Epididymis and Vas Deferens of Yaks (Bos grunniens)[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1925-1933.

References 26

[1]	CLIFTON V L, HUSBAND A J, KAY D J. Local immunity in the male reproductive tract[J]. Immunol Cell Biol, 1992, 70(5): 301-307.
[2]	CHAN P T K, SCHLEGEL P N. Inflammatory conditions of the male excurrent ductal system. Part I[J]. J Androl, 2002, 23(4): 453-460.
[3]	CAO S Z, LI J S, ZHANG Y, et al. Expression profile of TLRs gene in the reproductive organs of male Bos grunniens[J]. Chinese Veterinary Science, 2014, 44(2): 193-198. (in Chinese)曹随忠, 李计尚, 张于, 等. TLRs基因在牦牛雄性生殖器官中表达情况的检测[J]. 中国兽医科学, 2014, 44(2): 193-198.
[4]	QIU Q, ZHANG G J, MA T, et al. The yak genome and adaptation to life at high altitude[J]. Nat Genet, 2012, 44(8): 946-949.
[5]	YU S J, HUANG Y M, CHEN B X. Reproductive patterns of the yak. I. Reproductive phenomena of the female yak[J]. Br Vet J, 1993, 149(6): 579-583.
[6]	JOHANSSON M, LYCKE N Y. Immunology of the human genital tract[J]. Curr Opin Infect Dis 2003, 16(1): 43-49.
[7]	EL-DEMIRY M I M, HARGREAVE T B, BUSUTTIL A, et al. Lymphocyte sub-populations in the male genital tract[J]. Brit J Urol, 1985, 57(6): 769-774.
[8]	ANDERSON D J, PUDNEY J. Human male genital tract immunity[M]//MESTECKY J, STROBER W, RUSSELL M W, et al. Mucosal Immunology. 4th ed. Amsterdam: Elsevier, 2015: 2125-2140.
[9]	HUMPHREY J D, LADDS P W. A quantitative histological study of changes in the bovine testis and epididymis associated with age[J]. Res Vet Sci, 1975, 19(2): 135-141.
[10]	NASHAN D, MALORNY U, SORG C, et al. Immuno-competent cells in the murine epididymis[J]. Int J Androl, 1989, 12(1): 85-94.
[11]	GRIGORYEVA L, HILL E, DA SILVA N. Postnatal development of mononuclear phagocytes in the mouse epididymis[J]. FASEB J, 2013, 27(S1): 734.
[12]	WU Y Y, HIRSCHI K K. Tissue-resident macrophage development and function[J]. Front Cell Dev Biol, 2021, 8: 617879.
[13]	WANG M, YANG Y L, CANSEVER D, et al. Two populations of self-maintaining monocyte-independent macrophages exist in adult epididymis and testis[J]. Proc Natl Acad Sci U S A, 2021, 118(1): e2013686117.
[14]	VOISIN A, WHITFIELD M, DAMON-SOUBEYRAND C, et al. Comprehensive overview of murine epididymal mononuclear phagocytes and lymphocytes: unexpected populations arise[J]. J Reprod Immunol, 2018, 126: 11-17.
[15]	CLEVERS H, ALARCON B, WILEMAN T, et al. The T cell receptor/CD3 complex: a dynamic protein ensemble[J]. Annu Rev Immunol, 1988, 6: 629-662.
[16]	RITCHIE A W S, HARGREAVE T B, JAMES K, et al. Intraepithelial lymphocytes in the normal epididymis. A mechanism for tolerance to sperm auto-antigens?[J]. Brit J Urol, 1984, 56(1): 79-83.
[17]	VOISIN A, SAEZ F, DREVET J R, et al. The epididymal immune balance: a key to preserving male fertility[J]. Asian J Androl, 2019, 21(6): 531-539.
[18]	HEDGER M P. Immunophysiology and pathology of inflammation in the testis and epididymis[J]. J Androl, 2011, 32(6): 625-640.
[19]	BOOTH J S, TOAPANTA F R. B and T cell immunity in tissues and across the ages[J]. Vaccines, 2021, 9(1): 24.
[20]	STERN J E, GARDNER S, QUIRK D, et al. Secretory immune system of the male reproductive tract: effects of dihydrotestosterone and estradiol on IgA and secretory component levels[J]. J Reprod Immunol, 1992, 22(1): 73-85.
[21]	TANAKA T, ICHIMURA K, SATO Y, et al. Frequent downregulation or loss of CD79a expression in plasma cell myelomas: potential clue for diagnosis[J]. Pathol Int, 2009, 59(11): 804-808.
[22]	MESTECKY J, RUSSELL M W. Induction of mucosal immune responses in the human genital tract[J]. FEMS Immunol Med Microbiol, 2000, 27(4), 351-355.
[23]	SIRIGU P, PERRA M T, MAXIA C, et al. IgM and IgG in the human male genital tract[J]. Arch Androl, 1997, 39(1): 45-53.
[24]	SIRIGU P, PERRA M T, TURNO F. Immunohistochemical study of secretory IgA in the human male reproductive tract[J]. Andrologia, 1995, 27(6): 335-339.
[25]	MOLDOVEANU Z, HUANG W Q, KULHAVY R, et al. Human male genital tract secretions: both mucosal and systemic immune compartments contribute to the humoral immunity[J]. J Immuno, 2005, 175(6): 4127-4136.
[26]	GUDISA R, GOYAL K, GUPTA P, et al. Localized and systemic immune response in human reproductive tract[J]. Front Cell Infect Microbiol, 2021, 11: 649893.

Distribution of Immune Cells in the Epididymis and Vas Deferens of Yaks (Bos grunniens)

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 26

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LIU Yili, TANG Jiao, MIN Qi, YANG Lu, WANG Zening, HU Lian, ZHAO Di, JIANG Mingfeng. Mining Key Candidate Genes of Development and Metabolism in Yak Abomasum Based on Transcriptome Data [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 153-168.
[2]	YAO Ying, ZHOU Yingcong, DU Peiyan, LI Yijuan, QIAN Wenjie, LI Liuyang, YU Zhipeng, CUI Yan, YU Sijiu, FAN Jiangfeng. Proteomic Analysis of Yak Serum During Pregnancy Based on TMT Technology [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 192-206.
[3]	ZHANG Ying, YUAN Ligang, CHEN Guojuan, ZHANG Fang, YANG Dapeng. Analysis of Differential Expression of ET-1/eNOS in the Development of Cryptorchidism in Yak [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 207-217.
[4]	LI Zaiwen, LI Xiang, LI Xiaowei, LI Biao, JIANG Mingfeng. The Pedigree Reconstruction of the Maiwa Yak Preserved Population Based on GBS Technology [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3710-3721.
[5]	WANG Jingyu, PAN Yangyang, XU Gengquan, ZHANG Rui, ZHANG Wenlan, WANG Xiaoshan, WU Rentaodi, ZHAO Rigetu, CUI Yan, YU Sijiu. Preparation and Preliminary Application of Yak (Bos Grunniens) Fas-associated Factor 1 Polyclonal Antibody [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3369-3382.
[6]	ZHANG Peng, WANG Mingxiu, JING Kemin, PENG Wei, TIAN Yuan, LI Yuqian, FU Changqi, SHU Shi, ZHONG Jincheng, CAI Xin. Abnormal Expression of FGFs/FGFRs and Their Mediated Signaling Pathway Genes Affect the Proliferative Activity of Undifferentiated Spermatogonia in Cattleyak [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2886-2897.
[7]	JIAO Zhengxing, PAN Yangyang, WANG Meng, WANG Jinglei, MA Wenbin, GAO Xiang, ZHANG Hui, CUI Yan, YU Sijiu, WANG Libin. Preparation of Polyclonal Antibody against Yak LC3B Protein and Its Application in Detection of Expression in Reproductive Organs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2436-2447.
[8]	CHANG Yiming, TANG Cheng, YUE Hua. Molecular Epidemiological Investigation of Bovine Respiratory Syncytial Virus in Yaks [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2030-2041.
[9]	MENG Zhaoyi, WANG Yunlu, XU Yefen, NIU Jiaqiang, SUOLANG Sizhu, GUO Min, XI Guangyin. Construction of Yak lncRNA ENSBGRT00000000387.1 Lentivirus Vector and Its Effect on Apoptosis of Yak Follicular Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1058-1070.
[10]	ZHANG Qian, CUI Yan, YU Sijiu, HE Junfeng, PAN Yangyang, WANG Meng. Transcriptome Analysis of the Cerebral Cortex in Newborn and Adult Yaks (Bos grunniens) [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4605-4614.
[11]	ZHANG Qiqi, WANG Junmei, YUE Ziqi, GUO Yixin, SHI Liyuan, ZHANG Xiaohong, ZOU Huawei, PENG Quanhui, XUE Bai, WANG Lizhi, WANG Zhisheng, HU Rui. Effect of LPS on the Complement C3 Activation and ATP Production in the Rumen Epithelial Cells of Yak [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4664-4675.
[12]	HAN Xintong, MA Tenghe, LI Yunlei, FAN Shijie, CHEN Jilan, SUN Yanyan. Research Progress on Epididymis Function and Its Regulatory Mechanism on Post-testicular Sperm Maturation in Poultry [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 4-11.
[13]	YANG Baigao, HAO Haisheng, DU Weihua, ZHU Huabin, ZHAO Xueming. Advances in Research on Plateau Adaptation of Yak [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 12-23.
[14]	TANG Ziwen, CHENG Huaqin, LIU Dongju, PHAGMO Droma, YANG Xue, LI Jian, YIN Shi. The Protective Effect of Proanthocyanidins on Oxidative Damage of Yak Granulosa Cells Induced by Zearalenone [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3006-3017.
[15]	ZHANG Lan, YAO Yifan, QIN Anqi, LI Rui, ZHANG Yiyang, CHEN Shuwu, XU Jin, QIAO Zilin, YANG Kun. Proteomic Analysis for Yaks PASMCs under Hypoxia and Normoxia Conditions Based on TMT Technique [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2182-2193.