番鸭产蛋各期肝脂肪酸组成及AMPK信号通路基因表达研究

doi:10.11843/j.issn.0366-6964.2021.09.006

Abstract

Abstract: The aim of this study was to investigate the gene expression in AMP-activated protein kinase (AMPK) signaling pathway and fatty acid composition in the liver of Muscovy duck at different egg-laying stages, so as to illustrate the mechanism of lipid metabolism in response to egg-laying. Fifteen female Muscovy ducks were selected at pre-laying (22 weeks), early laying (30 weeks), laying (40 weeks) and post-laying (60 weeks) stages, respectively. Serum lipid levels were determined by automatic biochemical analyzer. Liver histological structure was observed by hematoxylin-eosin (HE) staining and Oil Red O staining, gene expression in AMPK pathway was determined by quantitative real-time PCR (qRT-PCR), and fatty acid composition was measured by gas chromatography-mass spectrometry (GC-MS). The results showed that the levels of total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), triglyceride (TG) and very low density lipoprotein cholesterol (VLDL-C) were significantly higher at 40 weeks than those at 22, 30 and 60 weeks (P<0.05). The levels of high density lipoprotein cholesterol (HDL-C) at 30 and 40 weeks were significantly lower than those at 22 and 60 weeks (P<0.05). Liver sections stained by HE and Oil Red O showed that the reticular formation was intact at 22 weeks, and lipid deposition significantly increased at 40 and 60 weeks (P<0.05). AMPKα1 was in a low expression level at 22, 30, 40 and 60 weeks, and was significantly lower than the expression of carnitine palmitoyltransferase-1 (CPT1) and fatty acid synthase (FAS) at different egg-laying stages(P<0.05). FAS expressed in a significantly high level at 22, 40 and 60 weeks (P<0.05). The expression levels of hydroxy-methylglutaryl coenzyme A reductase (HMGR), hepatocyte nuclear factor 4α (HNF4α), acetyl-CoA carboxylase 1 (ACC1) and sterol regulatory element binding protein 1 (SREBP1c) at 40 weeks were significantly higher than those at 22 and 30 weeks (P<0.05). Liver saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) were mainly composed of C16:0, C18:0, C18:1 and C20:2 n-6, accounted for 32%, 16%, 30% and 9% of the total fatty acids, respectively. The contents of C14:0 and C16:0 at 40 weeks were significantly higher than those at 22 weeks(P<0.05). The contents of C24:0, C20:2 n-6 and ΣPUFA at 60 weeks were significantly higher than those at 22 and 40 weeks (P<0.05). In conclusion, the liver synthesized large amount of long-chain fatty acids by up-regulating the expression of lipid synthesis genes, including FAS,during egg-laying. The synthesized lipids were deposited in liver and transported into serum thus increased the serum lipid level during laying.

Key words: Muscovy duck, serum lipid, liver, AMPK signaling pathway, fatty acid

CLC Number:

S834.2

ZHU Wenjun, CHEN Xingyong, LIU Le, LIU Zhengquan, ZHAO Yutong, GENG Zhaoyu. Fatty Acid Composition and Gene Expression of AMPK Signaling Pathway in Liver of Muscovy Duck at Different Egg-laying Stages[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2429-2438.

References 33

[1]	YE P F, LI M, LIAO W, et al.Hypothalamic transcriptome analysis reveals the neuroendocrine mechanisms in controlling broodiness of Muscovy duck (Cairina moschata)[J].PLoS One, 2019, 14(5):e0207050.
[2]	YE P F, GE K, LI M, et al.Egg-laying and brooding stage-specific hormonal response and transcriptional regulation in pituitary of Muscovy duck (Cairina moschata)[J].Poult Sci, 2019, 98(11):5287-5296.
[3]	朱文俊, 杨万里, 韦聪聪, 等.番鸭产蛋前期和产蛋期肝脏组织非靶向代谢组学比较分析[J].中国农业大学学报, 2020, 25(12):76-85.ZHU W J, YANG W L, WEI C C, et al.Comparative analysis of nontargeted metabolomics in liver tissue of Muscovy duck in pre-laying and laying stages[J].Journal of China Agricultural University, 2020, 25(12):76-85.(in Chinese)
[4]	WALZEM R L, HANSEN R J, WILLIAMS D L, et al.Estrogen induction of VLDLy assembly in egg-laying hens[J].J Nutr, 1999, 129(2S Suppl):467S-472S.
[5]	SCHMIDINGER B, WEIJLER A M, SCHNEIDER W J, et al.Hepatosteatosis and estrogen increase apolipoprotein O production in the chicken[J].Biochimie, 2016, 127:37-43.
[6]	RATNA W N, BHATT V D, CHAUDHARY K, et al.Estrogen-responsive genes encoding egg yolk proteins vitellogenin and apolipoprotein II in chicken are differentially regulated by selective estrogen receptor modulators[J].Theriogenology, 2016, 85(3):376-383.
[7]	LI H, WANG T A, XU C L, et al.Transcriptome profile of liver at different physiological stages reveals potential mode for lipid metabolism in laying hens[J].BMC Genomic, 2015, 16:763.
[8]	陈兴勇, 耿照玉, 廖望, 等.一种基于脂联素基因ADP鉴定鸭繁殖性状的分子标记及其应用:中国, 201711459759.6[P].2020-05-08.CHEN X Y, GENG Z Y, LIAO W, et al.Molecular marker for identifying duck reproduction trait based on adiponectin (ADP) gene and application of molecular marker:CN, 201711459759.6[P].2020-05-08.(in Chinese)
[9]	ALVES-BEZERRA M, COHEN D E.Triglyceride metabolism in the liver[J].Compr Physiol, 2017, 8(1):1-8.
[10]	TUMOVÁ E, TEIMOURI A.Fat deposition in the broiler chicken:a review[J].Sci Agric Bohemica, 2010, 41(2):121-128.
[11]	KNIGHT B L, HEBBACHI A, HAUTON D, et al.A role for PPARalpha in the control of SREBP activity and lipid synthesis in the liver[J].Biochem J, 2005, 389(Pt 2):413-421.
[12]	SKOP V, CAHOVÁ M, PAPÁČKOVÁ Z, et al.Autophagy-lysosomal pathway is involved in lipid degradation in rat liver[J]. Physiol Res, 2012, 61(3):287-297.
[13]	MA Z, LI H, ZHENG H, et al.MicroRNA-101-2-5p targets the ApoB gene in the liver of chicken (Gallus Gallus)[J].Genome, 2017, 60(8):673-678.
[14]	ALVARENGA R R, ZANGERONIMO M G, PEREIRA L J, et al.Lipoprotein metabolism in poultry[J].World's Poult Sci J, 2011, 67(3):431-440.
[15]	DE MORENTIN P B M, GONZÁLEZ C R, SAHA A K, et al.Hypothalamic AMP-activated protein kinase as a mediator of whole body energy balance[J].Rev Endocr Metab Disord, 2011, 12(3):127-140.
[16]	周华金, 胡希怡, 杨家昶, 等.热应激对肉仔鸡肝AMPKα1及脂肪代谢相关分子基因表达的影响[J].畜牧兽医学报, 2018, 49(1):102-110.ZHOU H J, HU X Y, YANG J C, et al.Effects of heat stress on gene expression of AMPKα1 and lipid metabolism related molecules in the liver of broiler chickens[J].Acta Veterinaria et Zootechnica Sinica, 2018, 49(1):102-110.(in Chinese)
[17]	National Research Council.Nutrient requirements of poultry[M].9th ed.Washington, DC:National Academy Press, 1994.
[18]	国家卫生和计划生育委员会, 国家食品药品监督管理总局.GB 5009.168-2016食品安全国家标准食品中脂肪酸的测定[S].北京:中国标准出版社, 2017.National Health and Family Planning Commission of the People's Republic of China, China Food and Drug Administration.GB 5009.168-2016 National food safety Stan-dard:determination of fatty acid in foods[S].Beijing:China Standard Press, 2017.(in Chinese)
[19]	陈兴勇, 赵宁, 张燕, 等.皖西白鹅育肥期肌肉脂肪酸组成及肝PPARα、FADS2和ME1基因表达规律的研究[J].畜牧兽医学报, 2017, 48(10):1912-1919.CHEN X Y, ZHAO N, ZHANG Y, et al.The fatty acid profile in muscles and expression of PPARα, FADS2 and ME1 genes in liver of Chinese Wanxi white geese in fattening period[J].Acta Veterinaria et Zootechnica Sinica, 2017, 48(10):1912-1919.(in Chinese)
[20]	VON ECKARDSTEIN A, NOFER J R, ASSMANN G.High density lipoproteins and arteriosclerosis:role of cholesterol efflux and reverse cholesterol transport[J].Arterioscler, Thromb, Vasc Biol, 2001, 21(1):13-27.
[21]	KISS R S, SNIDERMAN A.Shunts, channels and lipoprotein endosomal traffic:a new model of cholesterol homeostasis in the hepatocyte[J].J Biomed Res, 2017, 31(2):95-107.
[22]	OLZMANN J A, CARVALHO P.Dynamics and functions of lipid droplets[J].Nat Rev Mol Cell Biol, 2019, 20(3):137-155.
[23]	WANG Q, LIU S D, ZHAI A H, et al.AMPK-mediated regulation of lipid metabolism by phosphorylation[J].Biol Pharm Bull, 2018, 41(7):985-993.
[24]	NADA M A, ABDEL-ALEEM S, SCHULZ H.On the rate-limiting step in the β-oxidation of polyunsaturated fatty acids in the heart[J].Biochim Biophys Acta, 1995, 1255(3):244-250.
[25]	JOHNSON A L.Ovarian follicle selection and granulosa cell differentiation[J].Poult Sci, 2015, 94(4):781-785.
[26]	EBERLÉ D, HEGARTY B, BOSSARD P, et al.SREBP transcription factors:master regulators of lipid homeostasis[J].Biochimie, 2004, 86(11):839-848.
[27]	邢智洋, 张梦璐, 张菡, 等.沉默SCAP基因对奶牛乳腺上皮细胞脂滴的影响[J].畜牧兽医学报, 2019, 50(3):507-516.XING Z Y, ZHANG M L, ZHANG H, et al.Effect of silencing SCAP gene on lipid droplets in dairy mammary epithelial cells[J].Acta Veterinaria et Zootechnica Sinica, 2019, 50(3):507-516.(in Chinese)
[28]	KIM C W, ADDY C, KUSUNOKI J, et al.Acetyl CoA carboxylase inhibition reduces hepatic steatosis but elevates plasma triglycerides in mice and humans:a bedside to bench investigation[J].Cell Metab, 2017, 26(2):394-406.
[29]	HAMPTON R Y.Proteolysis and sterol regulation[J].Annu Rev Cell Dev Biol, 2002, 18:345-378.
[30]	YU D S, CHEN G, PAN M L, et al.High fat diet-induced oxidative stress blocks hepatocyte nuclear factor 4α and leads to hepatic steatosis in mice[J].J Cell Physiol, 2018, 233(6):4770-4782.
[31]	TVRZICKA E, KREMMYDA L S, STANKOVA B, et al.Fatty acids as biocompounds:their role in human metabolism, health and disease-a review.Part 1:classification, dietary sources and biological functions[J].Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 2011, 155(2):117-130.
[32]	TVRZICKÁ E, VECKA M, STAŇKOVÁ B, et al.Analysis of fatty acids in plasma lipoproteins by gas chromatography-flame ionization detection:quantitative aspects[J].Anal Chim Acta, 2002, 465(1-2):337-350.
[33]	ROSQVIST F, MCNEIL C A, PRAMFALK C, et al.Fasting hepatic de novo lipogenesis is not reliably assessed using circulating fatty acid markers[J].Am J Clin Nutr, 2019, 109(2):260-268.

Fatty Acid Composition and Gene Expression of AMPK Signaling Pathway in Liver of Muscovy Duck at Different Egg-laying Stages

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 33

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Haibo, ZHAN Jinshun, GU Zhiyong, CHEN Xinfeng, PAN Yue, JIA Haobin, ZHONG Xiaojun, LI Kairong, ZHAO Shengguo, HUO Junhong. Comparative Study on Meat Quality Characteristics of Three-Way Hybrid Sheep Charolais×Duper×Hu and Charolais×Australian White×Hu and Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 110-119.
[2]	ZHANG De'an, YANG Ruozhu, LIU Jie, LIU Dewu, DENG Ming, LIU Guangbin, SUN Baoli, GUO Yongqing, LI Yaokun. Expression Analysis of Transcriptome in the Liver of Chuanzhong Black Goats Fed with Silage Neolamarckia Cadamba Substitute for Silage Corn [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 232-244.
[3]	MA Yajun, JIAO Zhihui, LIU Xiaoning, LU Xiangyu, LIU Tao, WANG Yue, PIAO Chenxi, WANG Hongbin. Effects of Adipose-derived Mesenchymal Stem Cells on Pyroptosis of Miniature Pigs with Hepatic Ischemia-Reperfusion Combined with Hepatectomy [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 355-364.
[4]	ZHANG Yafeng, ZHU Bin, MA Chang, ZHANG Yuanshu. The Research on the Effects of ACE2 Activated by Diminazene Aceturate on Mitochondria in the Liver of Rats with Non-alcoholic Fatty Liver Disease [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3895-3904.
[5]	YUAN Heling, FANG Ci, HUANG Jinhu, WANG Xiaoming, XIAO Wenjun, LIU Ruiting, SHI Rongmei, WANG Liping. Effects of Allicin on mRNA Expression of CYP1A2 and Its Enzyme Kinetics in Broilers Liver Tissue [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3905-3915.
[6]	CHEN Xin, QIN Tong. mRNA Vaccine and Its Research Prospect in Zoonotic Diseases [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2732-2742.
[7]	ZHAO Yangfei, YU Yanghuan, WANG Jinming, ZHANG Jianhai, SUN Zilong, NIU Ruiyan, WANG Jundong. Effects of IL-17A Knockout on Fluoride-Induced Hepatic Inflammation and Hepatocyte Apoptosis [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 3108-3117.
[8]	ZHU Qian, CHENG Yating, LI Ruixuan, LI Chenjian, LIU Yating, KONG Xiangfeng. Effects of Probiotics and Synbiotics Addition to Sows’ Diet on Fatty Acid Composition and Related Gene Expression in Muscle of Offspring Bama Mini-Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2458-2467.
[9]	ZHAO Donghao, YUAN Meng, MA Kaiteng, DUAN Zhuo, ZHU Yixin, TANG Fang, HAN Keguang, HUO Nairui. Chelating Role of Sheep Bone Collagen Peptide to Cadmium and Its Protection Role against Liver Injuries Induced by Cadmium in Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2641-2652.
[10]	SUN Xiaojing, ZHANG Lei, TIAN Tian, MA Xi, YAO Jia, WANG Yang. Unravelling Toxoplasma Treatment: Conventional Drugs toward Nanomedicine [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1834-1844.
[11]	OU Zhengmiao, ZHOU Jiawen, LIU Lili, WU Yun, CHEN Fenfen. Screening and Expression Analysis of Genes Related to Lipid Metabolism in Liver Tissue of Wuliangshan Sooty Chicken Based on RNA-Seq [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 976-988.
[12]	YAN Qiongxian, CHEN Wenxun, HUI Haoyang, PENG Can, TANG Shaoxun, ZHOU Xiaoling, TAN Zhiliang. Effects of Zearalenone on Growth Performance, Gastrointestinal Fermentation and Microbiota Community Structure of Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1109-1123.
[13]	SUN Meijie, CAO Liwen, ZHENG Wenjin, SHEN Junshi, ZHU Weiyun. Effect of Dietary Urea Supplementation on Liver Ammonia Metabolism in Fattening Hu Lambs Based on Transcriptome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1148-1159.
[14]	CHEN Jingyi, YU Miao, ZHANG Jinyang, FAN Kun, YANG Guijun, GE Ming, ZHANG Ruili. Study on the Involvement of Ferroptosis in Liver Injury of Cadmium-exposed Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 787-802.
[15]	ZHAO Huiying, YU Shiqiang, ZHAO Yuchao, JIANG Linshu. Mechanism of Liver-Adipose Tissue Crosstalk in the Development of Fatty Liver in Periparturient Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4105-4116.