丹皮酚对L-精氨酸诱导的小鼠急性胰腺炎氧化损伤的影响

doi:10.11843/j.issn.0366-6964.2021.07.020

Abstract

Abstract: To investigate the effect of paeonol on oxidative damage in mice with acute pancreatitis(AP), 50 SPF male mice were selected and randomly divided into 5 groups with 10 each, namely the blank control group, AP model group and paeonol high-, medium- and low- dose groups. The mice in the paeonol groups were given 100, 50, and 25 mg·kg^-1 paeonol intragastrically, and the mice in the blank control group and the AP model group were given equal volumes of saline. After continuously intragastric administration for 5 days, mice in the AP model group and different doses of paeonol groups were injected intraperitoneally with 20% L-arginine. After 6 hours of injection, serum samples of mice were taken to measure the oxidation indexes (MDA, SOD), and pancreatic tissues of mice were taken to observe the pathological changes. At the same time, myeloperoxidase(MPO) immunohistochemical sections of pancreatic tissue were made to observe the histopathological changes and the distribution and expression of MPO. The mRNA expression levels of HO-1,Keap1 and Nrf2 in pancreatic tissues of each group were detected by fluorescence quantitative PCR, and the protein expression levels of HO-1,Keap1 and Nrf2 were detected by Western blot. The results showed that different doses of paeonol significantly reduced the MDA content and increased the activity of SOD in mice with acute pancreatitis (P<0.01). Immunohistochemical results showed that high and medium dose of paeonol effectively reduced the expression of MPO. Compared with the model group, medium and high dose of paeonol significantly increased the mRNA and protein expression of HO-1 and Nrf2 (P<0.01), low dose of paeonol significantly increased the mRNA and protein expression of Nrf2(P<0.01). Different doses of paeonol significantly decreased the expression of Keap1 mRNA and protein(P<0.01). In conclusion, paeonol may alleviate oxidative damage induced by L-arginine in mice with acute pancreatitis by activating Nrf2/HO-1 signaling pathway. This study provides theoretical reference for the pathogenesis of acute pancreatitis and the development of related drugs.

Key words: paeonol, oxidative injury, pancreatitis, L-arginine, mechanism

CLC Number:

S856.4

ZHANG Peng, LI Yufu, WU Shangze, JIN Shengqi, WANG Jiaqing, Короткова Ирина Павловна, GUO Wenjie. Effects of Paeonol on the Oxidative Damage of Acute Pancreatitis in Mice Induced by L-Arginine[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(7): 1983-1990.

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Effects of Paeonol on the Oxidative Damage of Acute Pancreatitis in Mice Induced by L-Arginine

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