脂肪来源间充质干细胞条件培养基对小型猪腹腔镜肝损伤氧化应激反应的影响

doi:10.11843/j.issn.0366-6964.2021.06.028

Abstract

Abstract: To explore the oxidative stress response of adipose-derived mesenchymal stem cells-conditioned medium (ADSCs-CM) on liver injury in miniature pigs. In this experiment, 24 healthy miniature pigs were selected and randomly divided into four groups, namely model group (IRI), DMEM control group (DMEM), ADSCs-CM treatment group (CM) and ADSCs treatment Groups (ADSCs), six pigs each group. All four groups, laparoscopic techniques were used to establish the liver injury model of miniature pigs with ischemia reperfusion (IR) combined with partial hepatectomy. The pigs in the IRI group were transplanted with normal saline, the pigs in the DMEM group were transplanted with concentrated basal medium, and the pigs in the CM group were transplanted with concentrated adipose-derived stem cell culture medium, the pigs in the ADSCs group were transplanted with adipose stem cells. Blood and liver tissue samples were collected in each group before the operation, 1 day, 3 days and 7 days after the operation. Use liver function test kit to detect total bilirubin (T-BIL), lactate dehydrogenase (LDH), and total protein (TP) in sera; use oxidation reaction kit to detect malondialdehyde (MDA), intramedullary peroxidase (MPO), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase in liver tissue (GSH-Px) for detection. Results were as follows:1 day and 3 days after operation, the model group and the control group had severe liver function damage, with obvious oxidative stress. The CM and ADSCs treatment groups significantly promoted the recovery of liver function, and the expression of oxidative stress related indicators decreased significantly compared with the model group and the control group. After 7 days, each group basically recovered to the preoperative level. The results showed that laparoscopic liver ischemia-reperfusion combined with partial hepatectomy injury can cause oxidative stress in miniature pigs. Adipose-derived mesenchymal stem cells and their condition medium can improve oxidative stress after liver injury.

Key words: miniature pigs, laparoscopy, liver ischemia-reperfusion combined with partial hepatectomy injury, adipose stem cells-conditioned medium, oxidative stress

CLC Number:

S857.12

JIAO Zhihui, ZHANG Qianzhen, WANG Yue, LIU Tao, LIU Boyang, MA Yajun, LIU Xiaoning, PIAO Chenxi, WANG Hongbin. Effect of Adipose-derived Stem Cells-conditioned Medium on Oxidative Stress in Miniature Pigs with Laparoscopic Hepatic Injury[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6): 1734-1743.

References 31

[1]	曾利, 曹海平, 陈凯. 槲皮素对重要脏器缺血再灌注损伤保护作用的研究现状及进展[J]. 世界最新医学信息文摘, 2017, 17(94):22-23.ZENG L, CAO H P, CHEN K. Research status and progress of the protective effect of quercetin on ischemia-reperfusion injury of important organs[J]. World Latest Medicine Information (Electronic Version), 2017, 17(94):22-23. (in Chinese)
[2]	PANISELLO-ROSELLÓ A, ROSELLÓ-CATAFAU J. Molecular mechanisms and pathophysiology of ischemia-reperfusion injury[J]. Int J Mol Sci, 2018, 19(12):4093.
[3]	LOOR G, KONDAPALLI J, IWASE H, et al. Mitochondrial oxidant stress triggers cell death in simulated ischemia-reperfusion[J]. Biochim Biophys Acta Mol Cell Res, 2011, 1813(7):1382-1394.
[4]	JAESCHKE H, WOOLBRIGHT B L. Current strategies to minimize hepatic ischemia-reperfusion injury by targeting reactive oxygen species[J]. Transplant Rev (Orlando), 2012, 26(2):103-114.
[5]	SCHIEBER M, CHANDEL N S. ROS function in redox signaling and oxidative stress[J]. Curr Biol, 2014, 24(10):R453-R462.
[6]	DUBEY N K, MISHRA V K, DUBEY R, et al. Revisiting the advances in isolation, characterization and secretome of adipose-derived stromal/stem cells[J]. Int J Mol Sci, 2018, 19(8):2200.
[7]	NAHAR S, NAKASHIMA Y, MIYAGI-SHIOHIRA C, et al. Cytokines in adipose-derived mesenchymal stem cells promote the healing of liver disease[J]. World J Stem Cells, 2018, 10(11):146-159.
[8]	VAN POLL D, PAREKKADAN B, CHO C H, et al. Mesenchymal stem cell-derived molecules directly modulate hepatocellular death and regeneration in vitro and in vivo[J]. Hepatology, 2008, 47(5):1634-1643.
[9]	LI X T, MA T, SUN J C, et al. Harnessing the secretome of adipose-derived stem cells in the treatment of ischemic heart diseases[J]. Stem Cell Res Ther, 2019, 10(1):196.
[10]	王晓宇, 郭家辰, 刘勇. 脂肪间充质干细胞上清液对小鼠皮肤创伤的作用及可能机制[J]. 解剖科学进展, 2015, 21(3):263-266.WANG X Y, GUO J C, LIU Y. The effect and possible mechanism of supernatant of adipose mesenchymal stem cells on the skin wound healing in mice[J]. Progress of Anatomical Sciences, 2015, 21(3):263-266. (in Chinese)
[11]	NIADA S, GIANNASI C, GOMARASCA M, et al. Adipose-derived stromal cell secretome reduces TNFα-induced hypertrophy and catabolic markers in primary human articular chondrocytes[J]. Stem Cell Res, 2019, 38:101463.
[12]	HAJMOUSA G, PRZYBYT E, PFISTER F, et al. Human adipose tissue-derived stromal cells act as functional pericytes in mice and suppress high-glucose-induced proinflammatory activation of bovine retinal endothelial cells[J]. Diabetologia, 2018, 61(11):2371-2385.
[13]	张千振, 葛延松, 焦智慧, 等. 小型猪腹腔镜肝缺血再灌注合并肝部分切除对肝细胞凋亡及相关基因影响的研究[J]. 中国兽医杂志, 2018, 54(4):22-25, 28.ZHANG Q Z, GE Y S, JIAO Z H, et al. Effect of laparoscopic ischemia reperfusion combined with partial hepatectomy on hepatocyte apoptosis and related gene expression in miniature pigs[J]. Chinese Journal of Veterinary Medicine, 2018, 54(4):22-25, 28. (in Chinese)
[14]	李辉, 葛延松, 白鸽, 等. 富氢生理盐水干预小型猪肝脏缺血再灌注合并肝脏部分切除损伤中内质网应激影响的研究[J]. 中国畜牧兽医, 2018, 45(6):1692-1699.LI H, GE Y S, BAI G, et al. Hydrogen-rich saline protects effect against liver ischemia-reperfusion injury and hepatectomy by inhibiting endoplasmic reticulum stress in miniature pig[J]. China Animal Husbandry & Veterinary Medicine, 2018, 45(6):1692-1699. (in Chinese)
[15]	JIAO Z H, LIU X N, MA Y J, et al. Adipose-derived stem cells protect ischemia-reperfusion and partial hepatectomy by attenuating endoplasmic reticulum stress[J]. Front Cell Dev Biol, 2020, 8:177.
[16]	JIAO Z H, MA Y J, LIU X N, et al. Adipose-derived stem cell transplantation attenuates inflammation and promotes liver regeneration after ischemia-reperfusion and hemihepatectomy in swine[J]. Stem Cells Int, 2019, 2019:2489584.
[17]	BEER L, MILDNER M, ANKERSMIT H J. Cell secretome based drug substances in regenerative medicine:when regulatory affairs meet basic science[J]. Ann Transl Med, 2017, 5(7):170.
[18]	PITTENGER M F, MARTIN B J. Mesenchymal stem cells and their potential as cardiac therapeutics[J]. Circ Res, 2004, 95(1):9-20.
[19]	ABAZOV V, ABBOTT B, ACHARYA B, et al. Precision measurement of the ratio B (t→ Wb)/B (t→ Wq) and extraction of Vtb[J]. Phys Rev Lett, 2011, 107(12):121802.
[20]	ZANGI L, MARGALIT R, REICH-ZELIGER S, et al. Direct imaging of immune rejection and memory induction by allogeneic mesenchymal stromal cells[J]. Stem Cells, 2009, 27(11):2865-2874.
[21]	BAGLIO S R, PEGTEL D M, BALDINI N. Mesenchymal stem cell secreted vesicles provide novel opportunities in (stem) cell-free therapy[J]. Front Physiol, 2012, 3:359.
[22]	牛杨, 申才良, 宋旆文, 等. BMSC-CM通过抑制Notch1信号通路减轻H2O2诱导的神经干细胞凋亡[J]. 安徽医科大学学报, 2018, 53(6):845-850.NIU Y, SHEN C L, SONG P W, et al. BMSC-CM reduces the effects of oxidative stress damage on neural stem cells by inhibiting Notch1 signaling pathway[J]. Acta Universitatis Medicinalis Anhui, 2018, 53(6):845-850. (in Chinese)
[23]	秦森, 张枫惠, 朱自欣, 等. 诱导性多能干细胞条件培养基对成肌细胞氧化应激损伤的保护作用[J]. 黑龙江畜牧兽医, 2017(11):32-34, 291.QIN S, ZHANG F H, ZHU Z X, et al. Protective effect of induced pluripotent stem cell-conditioned medium on oxidative stress injury of myoblasts[J]. Heilongjiang Animal Science and Veterinary Medicine, 2017(11):32-34, 291. (in Chinese)
[24]	徐雪晶, 李栋, 李雪, 等. 间充质干细胞条件培养基治疗肝氧化应激损伤中部分miRNA的变化[J]. 中国病理生理杂志, 2016, 32(9):1670-1676.XU X J, LI D, LI X, et al. MicroRNA expression in hepatocytes with hydrogen peroxide-induced oxidative stress-injury and alleviating effect of mesenchymal stem cell-conditioned medium[J]. Chinese Journal of Pathophysiology, 2016, 32(9):1670-1676. (in Chinese)
[25]	GE Y S, ZHANG Q Z, JIAO Z H, et al. Adipose-derived stem cells reduce liver oxidative stress and autophagy induced by ischemia-reperfusion and hepatectomy injury in swine[J]. Life Sci, 2018, 214:62-69.
[26]	王新党, 张建军, 叶奎. 人羊膜间充质干细胞移植修复肝脏缺血-再灌注损伤[J]. 中国组织工程研究, 2016, 20(45):6788-6794.WANG X D, ZHANG J J, YE K. Human amniotic mesenchymal stem cell transplantation for the repair of liver ischemia-reperfusion injury[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(45):6788-6794. (in Chinese)
[27]	葛延松. 小型猪ADSCs对腹腔镜肝脏I/R合并部分切除损伤保护作用的研究[D]. 哈尔滨:东北农业大学, 2019.GE Y S. Study of the protective effect of miniature pig adipose derived stem cells on hepatic Ischemia-Reperfusion combined with laparoscopic epatectomy injury[D]. Harbin:Northeast Agricultural University, 2019. (in Chinese)
[28]	叶松庆, 李永全. 神经干细胞条件培养基对氧化应激损伤小鼠皮层神经元的作用[J]. 中国中西医结合外科杂志, 2018, 24(4):466-471.YE S Q, LI Y Q. Effect of neural stem cell-conditioned medium on mice cortical neurons injured by oxidative stress[J]. Chinese Journal of Surgery of Integrated Traditional and Western Medicine, 2018, 24(4):466-471. (in Chinese)
[29]	WEN C Y, HUANG C Q, YANG M, et al. The secretion from bone marrow mesenchymal stem cells pretreated with berberine rescues neurons with oxidative damage through activation of the keap1-Nrf2-HO-1 signaling pathway[J]. Neurotox Res, 2020, 38(1):59-73.
[30]	XU X J, LI D, LI X, et al. Mesenchymal stem cell conditioned medium alleviates oxidative stress injury induced by hydrogen peroxide via regulating miR143 and its target protein in hepatocytes[J]. BMC Immunol, 2017, 18(1):51.
[31]	HONG H E, KIM O H, KWAK B J, et al. Antioxidant action of hypoxic conditioned media from adipose-derived stem cells in the hepatic injury of expressing higher reactive oxygen species[J]. Ann Surg Treat Res, 2019, 97(4):159-167.

Effect of Adipose-derived Stem Cells-conditioned Medium on Oxidative Stress in Miniature Pigs with Laparoscopic Hepatic Injury

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 31

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG Xinting, QIU Wenyue, PANG Xiaoyue, SU Yiman, YE Jiali, HUANG Jianjia, ZHOU Shuilian, TANG Zhaoxin, WANG Rongmei, SU Rongsheng. Effect of Asiatic Acid Alleviating Myocardial Injury Caused by Lipopolysaccharide through Inhibiting Oxidative Stress and Ferroptosis in Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1787-1799.
[2]	JIANG Lijun, ZONG Yunhe, LI Yunlei, CHEN Jilan, GENG Zhaoyu, SUN Yanyan, JIN Sihua. Research Progress of Antioxidant Application in Poultry Semen Storage [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 913-923.
[3]	WANG Xin, NIE Tong, LI Aqun, MA Jun. Hesperidin Alleviates High-fat-diet Induced Hepatic Oxidative Stress in Mice via Oxidative Phosphorylation Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1302-1313.
[4]	CHEN Hong, RUAN Hongri, MA Tianwen, LI Yanan, MIAO Xue, YANG Wenyue, GAO Li, WEI Chengwei. The Mechanism of Puerarin Improving Cartilage Degeneration in PTOA Rats by Interfering with Oxidative Stress and Nrf2/HO-1 Pathway of Cartilage [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3951-3963.
[5]	LIAN Yuju, ZHANG Zhiyuan, LIAO Xiaobo, WEI Hongjiang, YIN Yulong, LIU Mei. Breeding Methods and Application Progress of Medical Miniature Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2667-2682.
[6]	MAO Peng, WANG Zhihao, LI Jianji, CUI Luying, ZHU Guoqiang, MENG Xia, DONG Junsheng, WANG Heng. Research Progress of Ferroptosis in Bacterial Infection [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2280-2287.
[7]	HAN Xiuyuan, ZHAO Liang, WANG Chuang, QI Meiyu, YAO Yuchang. Nicotinic Acid Enhances Low Temperature Preservation of Sheep Sperm by Reducing Oxidative Stress Levels [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1979-1989.
[8]	CHEN Yongping, KOU Yuhong, JIAO Wenjing, HOU Xiaoyu, FAN Honggang. Effect of Coenzyme Q10 on LPS-induced Acute Lung Injury in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1730-1741.
[9]	XIAO Shiyu, LU Chang, MA Juan, WANG Chuang, QI Meiyu, YAO Yuchang. Effects of N-acetylcysteine [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1046-1057.
[10]	PAN Chanyuan, ZHAO Zixuan, DUAN Mingjie, JIANG Linshu, TONG Jinjin. The Mechanism of Artemisia carvifolia Alleviating Dairy Cow Oxidative Stress Predicted by Network Pharmacology [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1071-1084.
[11]	YANG Chengying, WANG Kai, HUANG Ziqing, LIN Hailan, WANG Naixiu, LI Yuhang, LIU Yanqing, LIU Yuxuan, ZHU Yan, HE Daoling, CHEN Hongyue, GAN Ling. Study on the Inhibitory Effect of Chito-oligosaccharide on Oxidative Stress inHippocampus of Piglets and Its Mechanism [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 744-756.
[12]	YANG Haotian, CHEN Yongping, WANG Zhiqiang, HUANG Yuxiang, MA Zhigang, ZOU Yue, WEI Niandong, ZHANG Hong, LI Xin, DONG Jiaqiang, LÜ Mingzhe, LI Hongbin, LIU Liwei, YANG Haoxuan, ZHANG Guohua, LIU Xuesong, ZHONG Peng, SHI Heye, KOU Yuhong, CHEN Zhifeng. Dexmedetomidine Inhibits NOX4 and Alleviates Acute Stress-induced Renal Injury in Rats [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 779-786.
[13]	LI Guangxing, CHEN Yang, CHEN Kaiting, WU Menglin, ZHANG Di, HUANG Xiaodan. Di (2-ethylhexyl) Phthalate Induces the Apoptosis and Necroptosis of HD11 Cells through the ROS/PTEN/PI3K/AKT Axis [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5240-5251.
[14]	LI Chunyan, ZHANG Yan, Lü Chunrong, DENG Weidong, QUAN Guobo. Research Progress on Antioxidant Mechanisms of Melatonin and Its Application in Cryopreservation of Mammalian Spermatozoa [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4468-4476.
[15]	YANG Mengran, YANG Chenglin, WU You, WANG Siqi, KONG Xiangyi, NING Can, XIAO Wenguang, FAN Hui, WU Jing, YUAN Zhihang. Study on the Mechanism of Citrinin Induced Intestinal Barrier Dysfunction in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4805-4816.