医用小型猪选育方法和应用进展

doi:10.11843/j.issn.0366-6964.2023.07.001

Abstract

Abstract: Model animals are widely used in human diseases research. With the development of research on this field, classical model animals, such as rodents, dogs, monkeys, can not meet the requirements for human medical research, then miniature pigs have gradually been recognized as an effective supplement to medical model animals. This review introduces the basic biological characteristics of miniature pigs, including anatomical and physiological characteristics, and discusses the advantages of them as medical model animals. Moreover, the main characteristics, origins, breeding methods, and application progress of 18 different breeds of miniature pigs, both in domestic and abroad, are introduced. In addition, this paper also reviews the current research and application situation of miniature pigs as model animals in the fields such as cardiovascular diseases, metabolism, xenotransplantation, and so on. This review is expected to provide reference for the conservation, development and utilization of the miniature pigs' germplasm resources in China, to benefit for the further research on human diseases.

Key words: miniature pigs, model animal, selection methods, diseases of cardiovascular system

CLC Number:

S828

LIAN Yuju, ZHANG Zhiyuan, LIAO Xiaobo, WEI Hongjiang, YIN Yulong, LIU Mei. Breeding Methods and Application Progress of Medical Miniature Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2667-2682.

References 90

[1]	田勇.动物模型与生物医药研究[J].生物化学与生物物理进展, 2022, 49(5):827-828.TIAN Y.Animal model and biomedical research[J].Progress in Biochemistry and Biophysics, 2022, 49(5):827-828.(in Chinese)
[2]	HELKE K L, MEYERHOLZ D K, BECK A P, et al.Research relevant background lesions and conditions:ferrets, dogs, swine, sheep, and goats[J].ILAR J, 2021, 62(1-2):133-168.
[3]	DONANDT T, HINTZE S, KRAUSE S, et al.Isolation and characterization of primary DMD pig muscle cells as an in vitro model for preclinical research on duchenne muscular dystrophy[J].Life (Basel), 2022, 12(10):1668.
[4]	GROENEN M A M, ARCHIBALD A L, UENISHI H, et al.Analyses of pig genomes provide insight into porcine demography and evolution[J].Nature, 2012, 491(7424):393-398.
[5]	宋修春, 王磊, 李玉春.小型猪种质资源引进培育及生物学特性研究进展[J].中国畜禽种业, 2021, 17(5):89-90.SONG X C, WANG L, LI Y C.Research progress on introduction, cultivation and biological characteristics of miniature pig Germplasm resources[J].The Chinese Livestock and Poultry Breeding, 2021, 17(5):89-90.(in Chinese)
[6]	江雨航, 申玉建, 张其伟, 等.广西巴马小型猪全身CT影像学观察[J].南方农业学报, 2020, 51(3):677-685.JIANG Y H, SHEN Y J, ZHANG Q W, et al.Observation of whole body CT tomography of Guangxi Bama mini-pigs[J].Journal of Southern Agriculture, 2020, 51(3):677-685.(in Chinese)
[7]	靳二辉, 单同领, 张勇, 等.近交系五指山小型猪消化器官的解剖学研究[J].中国畜牧兽医, 2007, 34(12):66-69.JIN E H, SHAN T L, ZHANG Y, et al.The anatomic study on the digestive organs in the Wuzhishan Miniature Pig inbred-line[J].China Animal Husbandry & Veterinary Medicine, 2007, 34(12):66-69.(in Chinese)
[8]	郑必祥, 彭代智, 左海斌, 等.巴马小型猪与人真皮的组织形态学和生物力学基本特征比较研究[J].第三军医大学学报, 2010, 32(8):754-758.ZHENG B X, PENG D Z, ZUO H B, et al.Comparative study on dermal histomorphologic and biomechanical properties between Bama miniature pig and human[J].Acta Academiae Medicinae Militaris Tertiae, 2010, 32(8):754-758.(in Chinese)
[9]	李伊为, 张延英.实验动物学[M].3版.北京:科学出版社, 2022:44-261.LI Y W, ZHANG Y Y.Laboratory animal science[M].3rd ed.Beijing:Science Press, 2022:44-261.(in Chinese)
[10]	刘薇, 贾俊双, 唐华, 等.慢病毒载体法制备遗传工程猴和小型猪的现状及应用前景展望[J].中国实验动物学报, 2012, 20(5):84-89.LIU W, JIA J S, TANG H, et al.Genetically engineered monkeys and minipigs generated by lentivirus-mediated gene delivery:current status and application perspective[J].Acta Laboratorium Animalis Scientia Sinica, 2012, 20(5):84-89.(in Chinese)
[11]	SON Y B, BHARTI D, KIM S B, et al.Hematological patterns and histopathological assessment of Miniature Pigs in the experiments on human mesenchymal stem cell transplantation[J].Int J Med Sci, 2021, 18(5):1259-1268.
[12]	靳洪涛, 凡春荣, 李慧, 等.实验用五指山小型猪正常生理值测定[J].实验动物科学, 2007, 24(6):69-73.JIN H T, FAN C R, LI H, et al.Determination of clinical normal physiological parameters in Chinese Wuzhishan Pig[J].Laboratory Animal Science, 2007, 24(6):69-73.(in Chinese)
[13]	邓俊成, 苏小华, 吕明, 等.正常巴马小型猪与高血糖家系F5代血液生理生化指标比较研究[J].中国比较医学杂志, 2018, 28(10):36-41.DENG J C, SU X H, LYU M, et al.Comparison of blood physiological and biochemical indexes between normal Bama minipigs and an F5 generation with hyperglycemia[J].Chinese Journal of Comparative Medicine, 2018, 28(10):36-41.(in Chinese)
[14]	王爱德, 郭亚芬, 李柏, 等.巴马小型猪血液生理指标[J].上海实验动物科学, 2001, 21(2):75-78.WANG A D, GUO Y F, LI B, et al.Determination of some haemal physiological parameters in Bama Mini-Pig[J].Shanghai Laboratory Animal Science, 2001, 21(2):75-78.(in Chinese)
[15]	杨李厂, 周文兵, 丁隽, 等.四个品种小型猪血液学指标测定及比较[J].实验动物与比较医学, 2016, 36(6):441-446.YANG L C, ZHOU W B, DING J, et al.Investigation on biological characteristics of blood for four breeds of minipigs[J]. Laboratory Animal and Comparative Medicine, 2016, 36(6):441-446.(in Chinese)
[16]	DUAN Y, YAN X X, WEI L H, et al.Acute and subacute toxicity studies of CMICE-013, a novel iodinated rotenone-based myocardial perfusion tracer, in Sprague Dawley rats and Gottingen minipigs[J].Regul Toxicol Pharmacol, 2016, 80:195-209.
[17]	夏薇, 陈婷梅, 王霄霞.临床血液学检验技术[M].北京:人民卫生出版社, 2015:363.XIA W, CHEN T M, WANG X X.Clinical hematologic laboratory technology[M].Beijing:People's Medical Publishing House, 2015:363.(in Chinese)
[18]	KUZMA B A, SENEMAR S, RAMEZANLI T, et al.Evaluation of local bioavailability of metronidazole from topical formulations using dermal microdialysis:Preliminary study in a Yucatan mini-pig model[J].Eur J Pharm Sci, 2021, 159:105741.
[19]	AVERY J T, QIAO J Z, MEDEIROS E, et al.Bi-layered living cellular construct resulted in greater healing in an alloxan-induced diabetic porcine model[J].Int Wound J, 2023, 20(2):403-412.
[20]	AL-MASHHADI R H, AL-MASHHADI A L, NASR Z P, et al.Local pressure drives low-density lipoprotein accumulation and coronary atherosclerosis in Hypertensive Minipigs[J].J Am Coll Cardiol, 2021, 77(5):575-589.
[21]	JOHNSON L L, JOHNSON J, OBER R, et al.Novel receptor for advanced glycation end products-blocking antibody to treat diabetic peripheral artery disease[J].J Am Heart Assoc, 2021, 10(1):e016696.
[22]	HALECKER S, KRABBEN L, KRISTIANSEN Y, et al.Rare isolation of human-tropic recombinant porcine endogenous retroviruses PERV-A/C from Göttingen minipigs[J].Virol J, 2022, 19(1):30.
[23]	FOUBERT P, DOYLE-EISELE M, GONZALEZ A, et al.Development of a combined radiation and full thickness burn injury minipig model to study the effects of uncultured adipose-derived regenerative cell therapy in wound healing[J].Int J Radiat Biol, 2017, 93(3):340-350.
[24]	BERGEN W G.Pigs (Sus scrofa) in biomedical research[M]//WU G Y.Recent Advances in Animal Nutrition and Metabolism. Cham:Springer, 2022:335-343.
[25]	WANG S, LIU Y, FANG D, et al.The miniature pig:a useful large animal model for dental and orofacial research[J].Oral Dis, 2007, 13(6):530-537.
[26]	KAMEGAYA Y, FARINELLI W A, VILA ECHAGUE A, et al.Evaluation of photochemical tissue bonding for closure of skin incisions and excisions[J].Lasers Surg Med, 2005, 37(4):264-270.
[27]	DABURON F, VILLIERS P A, FAGNIEZ P L, et al.Research on the therapeutics of intense abdominal irradiations in pigs[J]. Radiat Environ Biophys, 1979, 17(1):67-83.
[28]	ISOBE A, TASAKI M, INOUE T, et al.Histopathological background data of the systemic organs of CLAWN miniature swine with coronary artery stent implantation[J].J Toxicol Pathol, 2017, 30(1):25-38.
[29]	NIU D, WEI H J, LIN L, et al.Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9[J].Science, 2017, 357(6357):1303-1307.
[30]	叶雷, 李红, 魏红江, 等.成年版纳微型猪近交系克隆猪的制备[J].畜牧兽医学报, 2012, 43(9):1491-1498.YE L, LI H, WEI H J, et al.Production of cloning adult Banna Miniature inbred pig[J].Acta Veterinaria et Zootechnica Sinica, 2012, 43(9):1491-1498.(in Chinese)
[31]	陈华, 刘亚千, 李春海, 等.三个品系小型猪Ⅱ型糖尿病模型的比较研究[J].实验动物科学, 2007, 24(6):49-55.CHEN H, LIU Y Q, LI C H, et al.Comparison of three strains of minipigs with induced type Ⅱ diabetes mellitus[J].Laboratory Animal Science, 2007, 24(6):49-55.(in Chinese)
[32]	李凯, 牟玉莲, 韩建林, 等.五指山小型猪近交系微卫星等位基因遗传规律的研究[J].畜牧兽医学报, 2009, 40(3):296-302.LI K, MU Y L, HAN J L, et al.Study on genetic variation of inbred families of Wuzhishan Miniature pig using microsatellite DNA loci[J].Acta Veterinaria et Zootechnica Sinica, 2009, 40(3):296-302.(in Chinese)
[33]	冯书堂, 李奎, 刘岚, 等.小型猪近交系新品种的培育与开发利用[J].农业生物技术学报, 2015, 23(2):274-280.FENG S T, LI K, LIU L, et al.Cultivation and application of miniature pig (Sus scrofa) Inbred[J].Journal of Agricultural Biotechnology, 2015, 23(2):274-280.(in Chinese)
[34]	王爱德, 兰干球, 郭亚芬.广西巴马小型猪的培育[J].实验动物科学, 2010, 27(1):60-63.WANG A D, LAN G Q, GUO Y F.Genetic breeding of Guangxi Bama mini pig[J].Laboratory Animal Science, 2010, 27(1):60-63.(in Chinese)
[35]	景志忠, 房永祥, 陈国华, 等.高原型实验小型猪——合作小型猪的培育与应用[J].甘肃畜牧兽医, 2021, 51(11):5-14.JING Z Z, FANG Y X, CHEN G H, et al.Breeding and application of plateau experimental miniature pig-cooperative miniature pig[J].Gansu Animal Husbandry Veterinarian, 2021, 51(11):5-14.(in Chinese)
[36]	赵继勋, 张国中.中国小型猪实验动物化研究[C]//中国生物多样性保护基金会.第四届生物多样性保护与利用高新科学技术国际研讨会论文集.北京:北京科学技术出版社, 2003:178-184.ZHAO J X, ZHANG G Z.Study on experimental animalization of Chinese miniature pigs[C]//China Biodiversity Conservation Foundation.Proceedings of the 4th International Symposium on Biodiversity Conservation and Utilization.Beijing:Beijing Science and Technology Press, 2003:178-184.(in Chinese)
[37]	HAMMER R E, PURSEL V G, REXROAD C E JR, et al.Production of transgenic rabbits, sheep and pigs by microinjection[J]. Nature, 1985, 315(6021):680-683.
[38]	吴梦, 刘作华, 林保忠, 等.转基因猪研究进展[J].中国生物工程杂志, 2015, 35(3):92-98.WU M, LIU Z H, LIN B Z, et al.Recent progress in transgenic pigs[J].China Biotechnology, 2015, 35(3):92-98.(in Chinese)
[39]	刘奂.Rosa26位点整合eGFP转基因猪的制备及eGFP在组织器官内的表达分析[D].长春:吉林大学, 2021.LIU H.Generation of eGFP pigs at Rosa26 site and analysis eGFP expression in various tissues[D].Changchun:Jilin University, 2021.(in Chinese)
[40]	ZHANG H X, ZHANG Y, YIN H.Genome editing with mRNA encoding ZFN, TALEN, and Cas9[J].Mol Ther, 2019, 27(4):735-746.
[41]	TOWNSEND J A, WRIGHT D A, WINFREY R J, et al.High-frequency modification of plant genes using engineered zinc-finger nucleases[J].Nature, 2009, 459(7245):442-445.
[42]	HSU P D, LANDER E S, ZHANG F.Development and applications of CRISPR-Cas9 for genome engineering[J].Cell, 2014, 157(6):1262-1278.
[43]	DOUDNA J A, CHARPENTIER E.The new frontier of genome engineering with CRISPR-Cas9[J].Science, 2014, 346(6213):1258096.
[44]	王欢, 邹惠影, 朱化彬, 等.CRISPR/Cas9基因编辑技术在家畜育种新材料创制中的研究进展[J].畜牧兽医学报, 2021, 52(4):851-861.WANG H, ZOU H Y, ZHU H B, et al.Advances in evaluation of livestock breeding new materials by using the CRISPR/Cas9 gene editing technology[J].Acta Veterinaria et Zootechnica Sinica, 2021, 52(4):851-861.(in Chinese)
[45]	WHITWORTH K M, ROWLAND R R R, EWEN C L, et al.Gene-edited pigs are protected from porcine reproductive and respiratory syndrome virus[J].Nat Biotechnol, 2016, 34(1):20-22.
[46]	SHI X, TANG T, LIN Q Y, et al.Efficient generation of bone morphogenetic protein 15-edited Yorkshire pigs using CRISPR/Cas9[J].Biol Reprod, 2020, 103(5):1054-1068.
[47]	陈雨荣, 安星兰, 汪正铸, 等.猪作为人类疾病模型的研究进展[J].中国比较医学杂志, 2020, 30(7):110-119.CHEN Y R, AN X L, WANG Z Z, et al.Research progress on pigs as human disease models[J].Chinese Journal of Comparative Medicine, 2020, 30(7):110-119.(in Chinese)
[48]	张燕华, 刘天斌, 曲庆美, 等.巴马小型猪植入式遥测动物模型的建立及应用[J].药物评价研究, 2022, 45(10):2049-2053.ZHANG Y H, LIU T B, QU Q M, et al.Establishment and application on implantable telemetry animal model of Bama miniature pigs[J].Drug Evaluation Research, 2022, 45(10):2049-2053.(in Chinese)
[49]	GOETZMAN E, GONG Z W, RAJASUNDARAM D, et al.Serum metabolomics reveals distinct profiles during ischemia and reperfusion in a porcine model of myocardial ischemia-reperfusion[J].Int J Mol Sci, 2022, 23(12):6711.
[50]	马国钤.cMyBP-C在猪急性心肌梗死模型中的研究[D].昆明:昆明医科大学, 2021.MA G Q.The study of cMyBP-C in porcine acute myocardial infarction model[D].Kunming:Kunming Medical University, 2021.(in Chinese)
[51]	田毅, 李巨波, 张宝杰, 等.经皮冠状动脉球囊结合凝胶海绵联合栓塞法建立急性心肌梗死小型猪模型方法探讨[J].心肺血管病杂志, 2020, 39(9):1122-1126, 1133.TIAN Y, LI J B, ZHANG B J, et al.Establishment of acute myocardial infarction model by percutaneous coronary balloon combined with gelfoam embolization in miniature pigs[J].Journal of Cardiovascular and Pulmonary Diseases, 2020, 39(9):1122-1126, 1133.(in Chinese)
[52]	LIU J X, SHANG X H, FU J H, et al.Effects of recombinant staphylokinase on coronary thrombosis in Chinese experimental miniature swine[J].Acta Pharmacol Sin, 2002, 23(6):509-515.
[53]	宋健, 赵磊, 刘挨师.小型猪心肌缺血模型的构建及应用现状[J].中国组织工程研究, 2023, 27(5):772-778.SONG J, ZHAO L, LIU A S.Construction and application of myocardial ischemia model in miniature pigs[J].Chinese Journal of Tissue Engineering Research, 2023, 27(5):772-778.(in Chinese)
[54]	MIYAGAWA S, MIZOGUCHI H, FUKUSHIMA S, et al.New regional drug delivery system by direct epicardial placement of slow-release prostacyclin agonist promise therapeutic angiogenesis in a porcine chronic myocardial infarction[J].J Artif Organs, 2021, 24(4):465-472.
[55]	LOPEZ D, PAN J A, POLLAK P M, et al.Multiparametric CMR imaging of infarct remodeling in a percutaneous reperfused Yucatan mini-pig model[J].NMR Biomed, 2017, 30(5):e3693.
[56]	傅向华, 刘晓堃, 谷新顺, 等.应用4F导管冠状动脉内注入塑料微球建立慢性心力衰竭小型猪模型[C]//中华医学会第11次心血管病学术会议论文摘要集.大连:中华医学会, 2009:318-319.FU X H, LIU X K, GU X S, et al.Establishment of a miniature pig model of chronic heart failure by intracoronary injection of plastic microspheres with 4F catheter[C]//Abstracts of the 11th Chinese Medical Association Conference on Cardiovascular Diseases. Dalian:Chinese Medical Association, 2009:318-319.(in Chinese)
[57]	SCHULERI K H, BOYLE A J, CENTOLA M, et al.The adult Gottingen minipig as a model for chronic heart failure after myocardial infarction:focus on cardiovascular imaging and regenerative therapies[J].Comp Med, 2008, 58(6):568-579.
[58]	刘建勋, 李欣志, 于震.疾病模型动物拟临床研究概念的形成及其在药理学研究中的应用——长期高脂饲料喂养加冠脉内皮损伤制备小型猪慢性心肌缺血模型的研究[C]//中国药理学会第十次全国学术会议专刊.天津:中国药理学会, 2009:123-126.LIU J X, LI X Z, YU Z.The formation of the concept of disease model animal quasi-clinical research and its application in pharmacological research-the preparation of chronic myocardial ischemia model in miniature pigs by long-term high-fat diet and coronary endothelial injury[C]//Special issue of the 10th National Academic Conference of Chinese Pharmacological Society.Tianjin:Chinese Pharmacological Society, 2009:123-126.(in Chinese)
[59]	李欣志.痰瘀互结证冠心病模型的病理机制及方证相应基础研究[D].北京:中国中医科学院, 2008.LI X Z.Explorations on the mechanisms of coronary heart diseases inmodels with the syndrome of phlegm and stagnated blood and the correlation between Fang and Zheng[D].Beijing:China Academy of Chinese Medical Sciences, 2008.(in Chinese)
[60]	MOHAMMAD M A, STONE G W, KOUL S, et al.On the Natural history of coronary artery disease:a longitudinal nationwide serial angiography study[J].J Am Heart Assoc, 2022, 11(21):e026396.
[61]	赵茜, 高宇, 杜思成, 等.2型糖尿病患者颈动脉粥样硬化程度与血清ADAM10水平的相关性[J].中国临床研究, 2021, 34(3):289-293.ZHAO X, GAO Y, DU S C, et al.Relationship between carotid atherosclerosis and serum ADAM10 level in patients with type 2 diabetes mellitus[J].Chinese Journal of Clinical Research, 2021, 34(3):289-293.(in Chinese)
[62]	JELLINGER P S.American association of clinical Endocrinologists/American college of endocrinology management of dyslipidemia and prevention of cardiovascular disease clinical practice guidelines[J].Diabetes Spectr, 2018, 31(3):234-245.
[63]	MAHMOOD S S, LEVY D, VASAN R S, et al.The Framingham Heart Study and the epidemiology of cardiovascular disease:a historical perspective[J].Lancet, 2014, 383(9921):999-1008.
[64]	HIRSCH A T, HARTMAN L, TOWN R J, et al.National health care costs of peripheral arterial disease in the Medicare population[J].Vasc Med, 2008, 13(3):209-215.
[65]	OLVER T D, GRUNEWALD Z I, GHIARONE T, et al.Persistent insulin signaling coupled with restricted PI3K activation causes insulin-induced vasoconstriction[J].Am J Physiol Heart Circ Physiol, 2019, 317(5):H1166-H1172.
[66]	林珍, 王琦, 周艳辉, 等.不同性别2型糖尿病患者动脉粥样硬化指标的影响因素研究[J].中国全科医学, 2021, 24(9):1066-1070.LIN Z, WANG Q, ZHOU Y H, et al.Sex-specific risk factors for indicators of atherosclerosis in patients with type 2 diabetes mellitus[J].Chinese General Practice, 2021, 24(9):1066-1070.(in Chinese)
[67]	沈利叶, 潘永明, 徐雁云, 等.高脂高糖饮食诱导五指山小型猪动脉粥样硬化模型肠道菌群的变化[J].中国实验动物学报, 2022, 30(3):299-308.SHEN L Y, PAN Y M, XU Y Y, et al.Changes in intestinal flora in a Wuzhishan minipig atherosclerosis modelinduced by high-fat and high-sugar diet[J].Acta Laboratorium Animalis Scientia Sinica, 2022, 30(3):299-308.(in Chinese)
[68]	郁晨.基于"肝-肠"轴探讨冠心宁片抗西藏小型猪AS的作用及机制[D].杭州:浙江中医药大学, 2020.YU C.Study on the mechanism of Guanxinning tablets on Tibet minipigs withatherosclerosis based on the "liver-gut" axis[D].Hangzhou:Zhejiang Chinese Medical University, 2020.(in Chinese)
[69]	潘嘉祥, 张哲, 尹妮, 等.高脂喂饲联合机械损伤术建立巴马小型猪冠状动脉粥样硬化模型[J].中华中医药杂志, 2020, 35(3):1207-1212.PAN J X, ZHANG Z, YIN N, et al.Establishment of coronary atherosclerosis model in Bama miniature pig byhigh-fat diet combined with mechanical injury[J].China Journal of Traditional Chinese Medicine and Pharmacy, 2020, 35(3):1207-1212.(in Chinese)
[70]	THIM T, HAGENSEN M K, DROUET L, et al.Familial hypercholesterolaemic downsized pig with human-like coronary atherosclerosis:a model for preclinical studies[J].EuroIntervention, 2010, 6(2):261-268.
[71]	AL-MASHHADI R H, SØRENSEN C B, KRAGH P M, et al.Familial hypercholesterolemia and atherosclerosis in cloned minipigs created by DNA transposition of a human PCSK9 gain-of-function mutant[J].Sci Transl Med, 2013, 5(166):166ra1.
[72]	LI L, ZHAO Z Z, XIA J H, et al.A long-term high-fat/high-sucrose diet promotes kidney lipid deposition and causes apoptosis and glomerular hypertrophy in Bama Minipigs[J].PLoS One, 2015, 10(11):e0142884.
[73]	ZHAO Y Q, NIU M M, JIA Y X, et al.Establishment of type 2 diabetes mellitus models using streptozotocin after 3 months high-fat diet in Bama Minipigs[J].Anim Biotechnol, 2022, doi:10.1080/10495398.2022.2088548.
[74]	谢忠忱, 黄广勇, 陈华, 等.五指山小型猪高血脂症模型的建立[C]//中国实验动物学会第七届学术年会论文集.桂林:中国实验动物学会, 2006:57.XIE Z C, HUANG G Y, CHEN H, et al.Establishment of hyperlipidemia model in Wuzhishan miniature pig[C]//Proceedings of the 7th Annual Academic Conference of Chinese Society of Laboratory Animals.Guilin:Chinese Association for Laboratory Animal Sciences, 2006:57.(in Chinese)
[75]	ATIQ F, VAN DE WOUW J, SOROP O, et al.Endothelial dysfunction, atherosclerosis, and increase of Von Willebrand factor and factor VIII:a randomized controlled trial in swine[J].Thromb Haemost, 2021, 121(5):676-686.
[76]	HUANG L, HUA Z D, XIAO H W, et al.CRISPR/Cas9-mediated ApoE-/- and LDLR-/- double gene knockout in pigs elevates serum LDL-C and TC levels[J].Oncotarget, 2017, 8(23):37751-37760.
[77]	BURKE A C, TELFORD D E, SUTHERLAND B G, et al.Bempedoic acid lowers low-density lipoprotein cholesterol and attenuates atherosclerosis in low-density lipoprotein receptor-deficient (LDLR+/- and LDLR-/-) Yucatan Miniature Pigs[J].Arterioscler Thromb Vasc Biol, 2018, 38(5):1178-1190.
[78]	张雪, 黄雷, 阮进学, 等.PCSK9基因D374Y突变体转基因猪的制备与分析[J].中国农业科学, 2017, 50(19):3808-3816.ZHANG X, HUANG L, RUAN J X, et al.Production of transgenic pigs over-expressed PCSK9 D374Y Mutant[J].Scientia Agricultura Sinica, 2017, 50(19):3808-3816.(in Chinese)
[79]	晏昆, 蒙凌, 陈玉芳.PPARγ基因多态性与动脉粥样硬化性脑梗死的相关性研究[J].中国现代药物应用, 2020, 14(9):31-33.YAN K, MENG L, CHEN Y F.Correlation between PPAR γ genetic polymorphism and atherosclerotic cerebral infarction[J]. Chinese Journal of Modern Drug Application, 2020, 14(9):31-33(in Chinese)
[80]	吴彩霞, 刘朝明, 颜泉梅, 等.糖尿病和心血管病相关的重要靶点基因PPARγ过表达转基因克隆猪模型的建立[J].中国兽医学报, 2020, 40(7):1366-1371.WU C X, LIU C M, YAN Q M, et al.Establishment of transgenic pig model of PPAR γ overexpression, an important target gene related to diabetes mellitus and cardiovascular disease[J].Chinese Journal of Veterinary Science, 2020, 40(7):1366-1371.(in Chinese)
[81]	程俊霖.GIPR功能显性抑制的2型糖尿病转基因猪模型的建立[D].南京:南京医科大学, 2014.CHENG J L.Establishment of type 2 diabetic transgenic pig model with dominant inhibition of GIPR function[D].Nanjing:Nanjing Medical University, 2014.(in Chinese)
[82]	DORANDEU F, MIKLER J R, THIERMANN H, et al.Swine models in the design of more effective medical countermeasures against organophosphorus poisoning[J].Toxicology, 2007, 233(1-3):128-144.
[83]	ZHAO Y C, WANG H Y, LI Y F, et al.The action of topical application of Vitamin B12 ointment on radiodermatitis in a porcine model[J].Int Wound J, 2023, 20(2):516-528.
[84]	KARLSSON M, YANG Z H, CHAWLA S, et al.Evaluation of diffusion tensor imaging and fluid based biomarkers in a large animal trial of cyclosporine in focal traumatic brain injury[J].J Neurotrauma, 2021, 38(13):1870-1878.
[85]	HELKE K L, SWINDLE M M.Animal models of toxicology testing:the role of pigs[J].Expert Opin Drug Metab Toxicol, 2013, 9(2):127-139.
[86]	ROSS M J, COATES P T.Using CRISPR to inactivate endogenous retroviruses in pigs:an important step toward safe xenotransplantation?[J].Kidney Int, 2018, 93(1):4-6.
[87]	HAWTHORNE W J.World first pig-to-human cardiac xenotransplantation[J].Xenotransplantation, 2022, 29(1):e12733.
[88]	MEHRA M R.Cardiac xenotransplantation:rebirth amidst an uncertain future[J].J Card Fail, 2022, 28(5):873-874.
[89]	FARR M, STEHLIK J.Heart xenotransplant:a door that is finally opening[J].Circulation, 2022, 145(12):871-873.
[90]	SHAH A M, HAN J J.First successful porcine to human heart transplantation performed in the United States[J].Artif Organs, 2022, 46(4):543-545.

Breeding Methods and Application Progress of Medical Miniature Pigs

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