凝结芽孢杆菌对肉鸡生长性能、血清生化指标和胫骨强度指标的影响

doi:10.11843/j.issn.0366-6964.2021.04.018

Abstract

Abstract: The present study was conducted to evaluate the effects of Bacillus Coagulans on growth performance, serum biochemical and tibia indexes of broilers. One hundred and eighty 1-day-old healthy Arbor Acres broilers were randomly allotted to three treatments (basal diet; basal diet + 5 mg·kg^-1 flavomycin, 75 mg·kg^-1 aureomycin and 20 mg·kg^-1 kitasamycin; basal diet + 3.6×10⁹ CFU·kg^-1 Bacillus Coagulans). The experiment lasted for 42 days. The results showed as follows: 1) Bacillus Coagulans supplementation significantly reduced the average daily feed intake of 1-21 day broilers(P<0.05), and no significant difference in BW and ADG were observed among the treatment groups (P>0.05). 2) Compared with the control group, the supplementation of Bacillus Coagulans significantly increased the serum concentrations of triglyceride, total cholesterol and alkaline phosphatase activity of 1-21 day-old broilers (P<0.05). 3) Compared with the control group and antibiotic group, the supplementation of Bacillus Coagulans significantly increased the serum concentrations of total protein, albumin and globulin of 1-21 day broilers (P<0.05), but significantly reduced the serum ammonia and aric acid content (P<0.05), and had no significant effect on serum concentrations of total protein and albumin of day 42 broilers, but reduced the concentrations of serum ammonia. 4) The supplementation of Bacillus Coagulans significantly increased the content of calcium in the tibia of day 21 and day 42 broilers (P<0.05), and no significant difference in tibia (day 21 and 42)ash and phosphorus (day 21) content were observed among the treatment groups (P>0.05). Moreover, the tibia strength was the highest on day 42 in Bacillus Coagulans supplementation group(P>0.05). In conclusion, Bacillus Coagulans can reduce the average daily feed intake and improve the efficiency of feed conversion without decreasing the daily weight gain of broilers in the early stage. Dietary supplementation with Bacillus Coagulans supplementation is also beneficial to promote the metabolism and turnover of lipids and proteins, and improve the deposition of calcium and phosphorus in the tibia of broilers.

Key words: Bacillus Coagulans, broilers, growth performance, serum biochemical indices, tibia indices

CLC Number:

S831.4

WU Zhengke, ZHANG Anrong, CHEN Zhimin, CHANG Wenhuan, LIU Guohua, CAI Huiyi, ZHENG Aijuan. Effects of Bacillus Coagulans on Growth Performance, Serum Biochemical Indices and Tibia Strength Indices of Broilers[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 1031-1041.

References 40

[1]	田志梅,蒋宗勇,王丽,等.益生菌的功能及其在生猪养殖产业中的应用[J].动物营养学报,2019,31(3):1020-1030.TIAN Z M,JIANG Z Y,WANG L,et al.Function and application of probiotics in pig breeding[J].Chinese Journal of Animal Nutrition,2019,31(3):1020-1030.(in Chinese)
[2]	夏涛,王乙茹,刘元述,等.凝结芽孢杆菌在肉鸡养殖中应用效果及其作用机理研究进展[J].中国家禽,2019,41(3):39-43.XIA T,WANG Y R,LIU Y S,et al.Advancement on effects and mechanism of Bacillus coagulans additive in broilers feeding[J].China Poultry,2019,41(3):39-43.(in Chinese)
[3]	BENSON K F,REDMAN K A,CARTER S G,et al.Probiotic metabolites from Bacillus coagulans GanedenBC30TM support maturation of antigen-presenting cells in vitro[J].World J Gastroenterol,2012,18(16):1875-1883.
[4]	HECK J X,FLÔRES S H,HERTZ P F,et al.Optimization of cellulase-free xylanase activity produced by Bacillus coagulans BL69 in solid-state cultivation[J].Process Biochem,2005,40(1):107-112.
[5]	ZHEN W R,SHAO Y J,GONG X Y,et al.Effect of dietary Bacillus coagulans supplementation on growth performance and immune responses of broiler chickens challenged by Salmonella enteritidis[J].Poult Sci,2018,97(8):2654-2666.
[6]	DEN BESTEN G,VAN EUNEN K,GROEN A K,et al.The role of short-chain fatty acids in the interplay between diet,gut microbiota,and host energy metabolism[J].J Lipid Res,2013,54(9):2325-2340.
[7]	林丽花,柯芙容,詹湉湉,等.凝结芽孢杆菌对黄羽肉鸡生产性能、血清生化指标及抗氧化功能的影响[J].动物营养学报,2014,26(12):3806-3813.LIN L H,KE F R,ZHAN T T,et al.Effects of Bacillus coagulans on performance,serum bioche-mical indices and antioxidant function of yellow broilers[J].Chinese Journal of Animal Nutrition,2014,26(12):3806-3813.(in Chinese)
[8]	PU J N,CHEN D W,TIAN G,et al.Effects of benzoic acid,Bacillus coagulans and oregano oil combined supplementation on growth performance,immune status and intestinal barrier integrity of weaned piglets[J].Anim Nutr,2020,6(2):152-159.
[9]	CAVAZZONI V,ADAMI A,CASTROVILLI C.Performance of broiler chickens supplemented with Bacillus coagulans as probiotic[J].Br Poult Sci,1998,39(4):526-529.
[10]	孙焕林,刘艳丰,王品,等.凝结芽孢杆菌对黄羽肉鸡生长性能、肠道功能及肉品质的影响[J].石河子大学学报:自然科学版,2014,32(3):307-312.SUN H L,LIU Y F,WANG P,et al.Effect of Bacillus coagulans on growth performance,intestinal function and its meat quality of yellow-feathered chicken[J].Journal of Shihezi University:Natural Science,2014,32(3):307-312.(in Chinese)
[11]	BAMI M K,AFSHARMANESH M,EBRAHIMNEJAD H.Effect of dietary Bacillus coagulans and different forms of Zinc on performance,intestinal microbiota,carcass and meat quality of broiler chickens[J].Probi Antimicrob Protns,2020,12(2):461-472.
[12]	LIN S Y,HUANG A T Y,LU J J.Effects of supplement with different level of Bacillus coagulans as probiotics on growth performance and intestinal microflora populations of broiler chickens[J].J Anim Vet Adv,2011,10(1):111-114.
[13]	HUNG A T,LIN S Y,YANG T Y,et al.Effects of Bacillus coagulans ATCC 7050 on growth performance,intestinal morphology,and microflora composition in broiler chickens[J].Anim Prod Sci,2012,52(9):874-879.
[14]	DAS S,SEN R.Kinetic modeling of sporulation and product formation in stationary phase by Bacillus coagulans RK-02 vis-à-vis other Bacilli[J].Bioresour Technol,2011,102(20):9659-9667.
[15]	JIANG T,XU Y B,SUN X C,et al.Kinetic characterization of recombinant Bacillus coagulans FDP-activated L-lactate dehydrogenase expressed in Escherichia coli and its substrate specificity[J].Protein Expr Purif,2014,95:219-225.
[16]	孙冶,李新鸣,王蔺,等.益生菌对肠道健康的功效及其作用机制的研究进展[J].沈阳医学院学报,2018,20(4):347-349,360.SUN Y,LI X M,WANG L,et al.Effects of probiotics on intestinal health and their mechanisms[J].Journal of Shenyang Medical College,2018,20(4):347-349,360.(in Chinese)
[17]	GIANG H H,VIET T Q,OGLE B,et al.Growth performance,digestibility,gut environment and health status in weaned piglets fed a diet supplemented with potentially probiotic complexes of lactic acid bacteria[J].Livest Sci,2010,129(1-3):95-103.
[18]	SOLDAVINI J,KAUNITZ J D.Pathobiology and potential therapeutic value of intestinal short-chain fatty acids in gut inflammation and obesity[J].Dig Dis Sci,2013,58(10):2756-2766.
[19]	肖英平,洪奇华,刘秀婷,等.谷氨酰胺对断奶仔猪生长性能、营养物质表观消化率、空肠碱性磷酸酶活性及与肠道健康相关因子基因表达的影响[J].动物营养学报,2012,24(8):1438-1446.XIAO Y P,HONG Q H,LIU X T,et al.Effects of glutamine on growth performance,apparent diges-tibility of nutrients,jejunal alkaline phosphatase activity and expression of genes related to intestinal health in weaner piglets[J].Chinese Journal of Animal Nutrition,2012,24(8):1438-1446.(in Chinese)
[20]	杨伟平,鄢珣,藏大存.不同品种鸭血清碱性磷酸酶活力与生长性能的关系研究[J].安徽农业科学,2010,38(16):8453-8454.YANG W P,YAN X,ZANG D C.Relationship between serum alkaline phosphatase activity and growth performance in different duck breeds[J].Journal of Anhui Agricultural Sciences,2010,38(16):8453-8454.(in Chinese)
[21]	赵娜,申杰,魏金涛,等.凝结芽孢杆菌对肉鸡生长性能、免疫器官指数、血清生化指标及肠道菌群的影响[J].动物营养学报,2017,29(1):249-256.ZHAO N,SHEN J,WEI J T,et al.Effects of Bacillus coagulans on growth performance,immune organ indexes,serum biochemical indexes and intestinal flora of broilers[J].Chinese Journal of Animal Nutrition,2017,29(1):249-256.(in Chinese)
[22]	李孟.总胆固醇、甘油三酯及其动态变化与代谢综合征关系的研究[D].大连:大连医科大学,2018.LI M.Associations of dynamic changes in total cholesterol,triglyceride with metabolic ssyndrome[D].Dalian:Dalian Medical University,2018.(in Chinese)
[23]	郑爱娟,吴正可,AHMED P S,等.丁酸梭菌对肉鸡生长性能和血清生化指标的影响[J].动物营养学报,2019,31(12):5519-5525.ZHENG A J,WU Z K,AHMED P S,et al.Effects of Clostridium butyricum on growth performance and serum biochemical indices of broilers[J].Chinese Journal of Animal Nutrition,2019,31(12):5519-5525.(in Chinese)
[24]	PETERS T JR.Serum albumin:recent progress in the understanding of its structure and biosynthesis[J].Clin Chem,2019,23(1):5-12.
[25]	张营营,郭茜,熊利霞,等.凝结芽孢杆菌对油鸡生长性能、血清指标及脏器系数的影响[J].北京农学院学报,2017,32(2):75-79.ZHANG Y Y,GUO Q,XIONG L X,et al.Effects of Bacillus coagulans on growth performance,serum indices and organ indicators of fatty chickens[J].Journal of Beijing University of Agriculture,2017,32(2):75-79.(in Chinese)
[26]	王乙茹,柳成东,白华毅,等.凝结芽孢杆菌与饲用抗生素组合使用对21~42日龄AA肉鸡生产性能的影响[J].中国畜牧杂志,2019,55(12):123-126.WANG Y R,LIU C D,BAI H Y,et al.Effects of combined use of Bacillus coagulans and feed antibiotics on the performance of 21~42 days old AA broilers[J].Chinese Journal of Animal Science,2019,55(12):123-126.(in Chinese)
[27]	程时军,马立保,张伟.溶菌酶对肉鸡肠粘膜形态、微生物数量及血氨浓度的影响[J].饲料工业,2009,30(20):13-16.CHENG S J,MA L B,ZHANG W.Effect of lysozyme on intestinal mucosal morphology,digesta microor-ganism and plasma concentration of ammonia for broilers[J].Feed Industry,2009,30(20):13-16.(in Chinese)
[28]	AMOROSO L,BARALDI A S M,BARREIRO F R,et al.Bone densitometry and calcium serum levels in chickens treated with filtered or unfiltered water[J].Rev Bras Cienc Avic,2013,15(4):379-384.
[29]	崔耀明,管军军,王金荣,等.益生乳酸菌对肉鸡骨骼发育的影响及作用机制研究进展[J].动物营养学报,2020,33(1):1-7.CUI Y M,GUAN J J,WANG J R,et al.Research progress on effects and mechanism of lactic acid bacteria on broilers' bone development[J].Chinese Journal of Animal Nutrition,2020,33(1):1-7.(in Chinese)
[30]	BERTAZZONI E,DONELLI G,MIDTVEDT T,et al.Probiotics and clinical effects:is the number what counts?[J].J Chemother, 2013,25(4):193-212.
[31]	CHARLES J F,ERMANN J,ALIPRANTIS A O.The intestinal microbiome and skeletal fitness:connecting bugs and bones[J].Clin Immunol,2015,159(2):163-169.
[32]	童小乐.TD肉鸡的肠道菌群变化及LPS对肉鸡胫骨形成的影响[D].武汉:华中农业大学,2019.TONG X L.The changes of gut microbial commnunity in chickens with TD and effects of LPS on the tiblal bone formation in chickens[D].Wuhan:Huazhong Agricultural University,2019.(in Chinese)
[33]	潘康成,杨汉博.饲用芽孢菌作用机理的研究进展[J].饲料工业,1997,18(9):32-34.PAN K C,YANG H B.Research progress on the mechanism of feed Bacillus[J].Feed Industry,1997,18(9):32-34.(in Chinese)
[34]	李叶涵,方山青,毕云天,等.益生菌对维生素K缺乏肉仔鸡骨骼健康的改善作用[J].中国家禽,2018,40(14):25-31.LI Y H,FANG S Q,BI Y T,et al.Effect of probiotics on bone health of broiler chickens fed vitamin K-deficient diet[J].China Poultry,2018,40(14):25-31.(in Chinese)
[35]	李叶涵.维生素K对肉鸡骨骼质量的调控作用[D].武汉:武汉轻工大学,2018.LI Y H.The effects of victamin K on bone quality of broilers[D].Wuhan:Wuhan Polytechnic University,2018.(in Chinese)
[36]	张显东.促生长抗生素作用机理及其替代方案的重新思考[J].饲料工业,2017,38(11):61-64.ZHANG X D.Rethinking about mechanism of antibiotic growth promoter and its alternatives[J].Feed Industry,2017,38(11):61-64.(in Chinese)
[37]	佟建明.饲用抗生素促生长机理研究进展[J].国外畜牧科技,1997,24(6):2-5.TONG J M.The researches on the mechanism of nutritional antibiotics[J].Animal Science Abroad,1997,24(6):2-5.(in Chinese)
[38]	NIEWOLD T A.The nonantibiotic anti-inflammatory effect of antimicrobial growth promoters,the real mode of action?a hypothesis[J].Poult Sci,2007,86(4):605-609.
[39]	UNNO T,KIM J,GUEVARRA R B,et al.Effects of antibiotic growth promoter and characterization of ecological succession in Swine gut microbiota[J].J Microbiol Biotechn,2015,25(4):431-438.
[40]	LUMPKINS B S,BATAL A B,LEE M D.Evaluation of the bacterial community and intestinal development of different genetic lines of chickens[J].Poult Sci,2010,89(8):1614-1621.

Effects of Bacillus Coagulans on Growth Performance, Serum Biochemical Indices and Tibia Strength Indices of Broilers

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