白头翁散煎剂发酵物对感染猪流行性腹泻病毒仔猪肠道屏障功能的影响

doi:10.11843/j.issn.0366-6964.2023.10.036

Abstract

Abstract: The trial aimed to study the effect of Pulsatilla Powder prescription on the intestinal barrier function of piglets infected with porcine epidemic diarrhea virus (PEDV). Twenty-four 7-day-old piglets (du×long×large) were randomly divided into 4 groups:control, PEDV, decoction and fermentation groups. The test period was 12 days. On day 4, piglets in the control group were inoculated with saline and the other three groups were inoculated with PEDV (HN2021) at a dose of 10^4.5 TCID₅₀ (50% tissue culture infection dose) per pig, and on days 5-9, the decoction and fermentation groups were orally given decoction and fermentation solution (0.2 mL·kg^-1) daily. To evaluate the growth performance, diarrhea index, viral copies, intestinal bacterial composition, intestinal morphology, intestinal barrier function of piglets, and antioxidant indicators in the serum and intestine. The results showed that the decoction and fermentation solution significantly increased the average daily gain (ADG), reduced diarrhea rate and inhibited viral replication in the intestine of the PEDV-infected piglets (P<0.05); and also regulated the intestinal bacteria composition of piglets, significantly increased the number of Lactobacillus and Bifidobacterium(P<0.05); the administration alleviated the significant decrease in intestinal villus height (VH) and increase in crypt depth (CD) in piglets caused by PEDV infection, improved intestinal morphology, significantly increased D-xylose content and decreased fatty acid binding protein (I-FABP) concentration in serum, thereby enhancing intestinal barrier function (P<0.05). In addition, the decoction and fermentation solution reduced oxidative stress, manifested by significantly increased total superoxide dismutase (T-SOD) activity and decreased malondialdehyde (MDA) content (P<0.05). These dates indicate Pulsatilla Powder can enhance intestinal barrier function in PEDV-infected piglets.

Key words: Pulsatilla Powder, fermentation, PEDV, growth performance, intestinal barrier function, antioxidant function

CLC Number:

S853.9

CHANG Weichen, LI Shuaiqi, LI Yan, YAN Wei, ZHANG Hongying, WANG Yanbin, YANG Mingfan, ZHANG Angke. Effect of Pulsatilla Powder Prescription Decoction Ferments on Intestinal Barrier Function of Piglets Infected with Porcine Epidemic Diarrhea Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4403-4410.

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Effect of Pulsatilla Powder Prescription Decoction Ferments on Intestinal Barrier Function of Piglets Infected with Porcine Epidemic Diarrhea Virus

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